Tjp1 (NM_001106266) Rat Tagged ORF Clone

CAT#: RR206456

Tjp1 (Myc-DDK-tagged ORF) - Rat tight junction protein 1 (Tjp1), (10 ug)

Product Images

Circular map for RR206456

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tjp1
• Synonyms: ZO-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206456 representing NM_001106266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAACAGCTATATGGGAACAGCACACAGTGACGCTTCACAGGGCTCCTGGGTTTGGATTTGGAA
  TTGCAATATCTGGTGGAAGAGATAATCCTCATTTTCAGAGTGGGGAAACCTCCATAGTGATTTCTGATGT
  GCTAAAAGGAGGGCCAGCTGAAGGACAGCTACAGGAAAATGACCGAGTCGCAATGGTTAACGGAGTTTCA
  ATGGATAATGTTGAACATGCTTTTGCTGTTCAGCAGCTAAGGAAAAGTGGGAAAAACGCAAAAATTACCA
  TCCGAAGAAAGAAGAAAGTTCAGATTCCTGTAAGTCACCCAGACCCTGACCCAGTGTCTGATAATGAAGA
  TGATAGCTATGACGAGGATGTGCACGATCCAAGAAGTGGCCGAGGTGCCCTAGCTAACAGAAGGGGTGAG
  AAGAGCTGGGCAAGGGATAGAAGCGCAAGCAGGGACCGGAGCCTGTCCCCTCGCTCAGACAGGCGATCAG
  TGGCCTCCAGTCAGCCCGCCAAACCCACCAAGGTCACATTGGTGAAGTCTCGGAAAAATGAAGAATATGG
  TCTTCGATTGGCCAGCCATATATTTGTAAAGGAAATTTCACAAGATAGTTTGGCAGCAAGAGATGGTAAC
  ATTCAAGAAGGGGATGTTGTCCTGAAGATAAATGGTACTGTGACAGAAAATATGTCACTGACAGATGCAA
  AAACATTGATAGAAAGGTCTAAAGGCAAGTTAAAAATGGTAGTGCAAAGAGATGAGCGGGCTACCTTATT
  GAATGTCCCTGATCTTTCAGATAGTATCCATTCTGCTAATGCCTCTGAAAGAGATGACATTTCAGAAATT
  CAGTCACTAGCATCAGACCATTCAGTTCGCTCCCATGACAGGCCTCCCCGCCGAAGCCAGTCACGATCTC
  CCGACCAACGTTCAGAGCCCTCTGATCATTCCACACAGTCTCCACAGCAGCCCAGCAATGGCAGTCTCCG
  GAGCAGAGAGGAAGAGCGCATGTCTAAACCCGGGGCTGTCTCAACTCCTGTAAAACATGTAGATGATCAT
  ACACCCAAAGCAGTGGAAGAAGTTACAGTTGAAAAACATGAGAAGCAGACACCCACTCTTCCAGAACCAA
  AACCTGTATATGCTCAAGTCGGACAGCCAGATGTGGATTTACCTGTCAGCCCTTCTGATGGTGTTCTGCC
  AAATTCAACACATGAAGATGGGATTCTTAGGCCCAGCATGAAACTGGTAAAATTCAGAAAAGGAGATAGT
  GTGGGTTTGCGACTAGCTGGTGGAAATGATGTCGGAATATTTGTAGCTGGCGTTCTAGAAGATAGCCCTG
  CAGCCAAAGAAGGCTTAGAGGAAGGTGATCAAATTCTCAGGGTGAACAATGTAGATTTCACAAATATCAT
  AAGAGAAGAAGCTGTTCTTTTCCTCCTCGACCTCCCTAAAGGTGAAGAAGTGACCATATTGGCTCAGAAG
  AAAAAGGACGTTTATCGCCGCATTGTAGAGTCAGATGTAGGAGACTCGTTCTATATTAGAACACATTTTG
  AATATGAGAAAGAATCTCCTTATGGACTCAGTTTTAACAAAGGAGAGGTGTTCCGGGTCGTGGACACCCT
  GTACAACGGGAAGCTGGGCTCCTGGCTGGCCATTCGAATTGGCAAAAATCACAAGGAGGTAGAGCGAGGC
  ATCGTTCCTAATAAGAACAGAGCCGAGCAGTTAGCCAGTGTACAGTACACACTTCCAAAGACAGCAGGTG
  GCGATCGGGCAGACTTCTGGAGGTTTCGAGGTCTTCGTAGCTCCAAGAGAAATCTTCGAAAAAGCAGAGA
  GGACCTGTCAGCGCAGCCAGTTCAAACAAAGTTCCCAGCTTATGAAAGGGTTGTTCTTCGAGAAGCTGGA
  TTCCTAAGACCTGTAACCATCTTTGGACCGATTGCTGATGTTGCCAGAGAAAAGTTAGCAAGGGAGGAGC
  CAGACATTTATCAGATTGCAAAAAGTGAACCACGAGATGCTGGAACTGACCATCGGAGCTCGGGCATTAT
  TCGCCTTCATACAATAAAGCAAATCATAGATCAAGATAAACATGCTTTATTAGATGTCACACCAAATGCA
  GTCGATCGCCTTAATTATGCACAGTGGTATCCGATTGTTGTGTTCCTTAACCCTGACTCTAAGCAGGGTG
  TAAAAACAATGAGGATGAGGCTATGTCCAGAGTCTCGGAAAAGTGCCAGGAAGCTATATGAACGGTCTCA
  TAAACTTCGTAAAAATAATCACCATCTTTTTACAACCACAATTAACTTAAACTCAATGAATGATGGTTGG
  TATGGTGCCCTGAAAGAAGCGATTCAGCAGCAACAGAACCAGCTGGTGTGGGTCTCTGAGGGGAAGGCGG
  ATGGTGCTACAAGTGATGACCTTGATTTGCATGATGATCGTCTGTCCTACCTGTCAGCCCCAGGTAGTGA
  GTACTCAATGTATAGCACGGACAGTAGACACACTTCTGACTATGAAGACACAGATACAGAAGGCGGGGCC
  TACACTGATCAAGAACTAGATGAAACTCTTAATGATGAGGTGGGGACTCCACCGGAGTCTGCTATTACAC
  GGTCCTCTGAGCCTGTAAGAGAGGATTCCTCTGGAATGCATCATGAAAACCAAACATATCCTCCTTACTC
  ACCACAAGCGCAGCCACAAGCTATTCATAGAATAGACTCCCCTGGACTTAAGACAGCCTCTCAACAGAAA
  GCAGAAGCCTCATCTCCAGTCCCTTACCTTTCGCCTGAAACAAACCCAGCATCATCAGCCTCTGCAGTTA
  AGCATAATGTAAATTTAACTAATGTCAACCTGGAGGAGCCCACCCCAGCTCCTCCCACCTCGCACGTATC
  ACAAGCTGATTGTTTAGGAGCACCAAGTCCTGAGGCACCTCACACGATGCTCAGAGACGAAGGAGTGTCA
  TTGCCGTCGCATGTAGACCCAGCAAAGGTATATAGGAAGGAGCCATATCCTGAGGAAATGATGAGACAAA
  ACCATATTTTAAAACAGCCAGCTCTTGGTCATCCAGGGCAGAGGCTGGATAAAGAGCCAAATCCAGCCTA
  TGATCCCCAACTTCCATATGTAGAAAAACAAGCCAGCAGAGACCTTGAGCAGCCACCATACAGGTATGAG
  TCCTCAAGCTACACAGACCAGTTTTCTCGGAACTATGACCATCGCCTACGATTTGAAGACCGAGTCCCTA
  CCTATGAAGACCAGTGGTCATATTATGATGACAAACAGCCCTACCCAACTCGGCCCTTTGATACTCAGCA
  TCCTCGAGACTTGGACTCCAGACAGCATCCTGAAGAGGCTTCAGAACGAGGCTATTTCCAGCGTTTTGAA
  GAGCCCGCCCCTCTGCCATATGACAGTAGACCACGCTACGAGCAGCTGCCTCGAACCTCTACTCTCCGAC
  ATGAAGAACAGCCAACCAGTGGATATGAGGTGCACAACAGGTACAGGCCAGAAGCACAGCCCTATGCTCC
  AGCAGGTCCTAAGTCATCTGAGCCGAAGCAGTACTTTGACCAGTACCCACGAAGTTATGAGCAAGTACCA
  CCACCAGGATTTACCTCCAAAACAGGCCATTACGAGCCTCTCCATGGTGCTGCAGTTGTTCCTCCTCTGA
  TACCTTCCTCTCAACATAAGCCAGAAGTTCTGCCCTCAGCTACCAAGCCACAGCCTCCGCCCCCAGCCCT
  AACTGAGGAAGAGGAGGATCCAGCAATGAAACCACAGTCTGTGCTCACCAGGGTCAAAATGTTTGAAAAC
  AAAAGATCTGCGTCTCTGGAGAACAAGAAGGATGTGAATGACACTGCCAGCTTTAAGCCTCCAGAAGTAG
  CATCTAAACCTCCAAGTGCTTCTCTTGTTGGCCCTAAACCTGTTTCTCAGACTCAGTTTAGTGAGCATGA
  CAAAACACTCTACAGGCTCCCAGAGCCTCAGAAACCTCAAGCGAAGCCACCTGAAGATATTGTTCGATCA
  AATCATTACGATCCTGAAGAGGATGAAGAGTATTACCGGAAACAGCTCTCTTACTTTGACCGGAGAAGTT
  TCGAGAGCAAGCCTCCTGCACATATTCCTGCTGGCCATCACTCAGAGCCTGCCAAGCCAGTCCATTCTCA
  GAGTCAGCCGAATTTCTCTAGTTATTCTTCAAAGGGGAAACCCGAAACTGATGCTATGGATAGATCATTC
  AGTGAGAAACGTTATGATCCAACCCAGGCCATGCCTCCTCCTCCTCCGTTGCCCTCACAGTACAGCCAGC
  CAGTTCCGCCTCTGTCCAACTCTTCTCTCCACATACATTCCAAGGCCGCCCAGAGTGAAGGCAATTCCGT
  ATCGTTGGATTTTCAGAACTCATATATATCCAAACCAGACCCACCCCCATCTCAGAGCAAACCAGCAACT
  TTTAGACCACCAACCCGGGAGGACCCTCCTCAGACCTTCTATCCCCAGAAAAGTTTCCCAGACAAAGCTT
  CAGTTAATGGAGCTGAGCAGACTCAGAAAACCATCACTCCAGCATACAACCGATTCACACCAAAGCCGTA
  CACGAGCTCTGCCCGGCCATTTGAACGCAAGTTTGAAAGTCCAAAGTTCAACCATAATCTTCTGCCAAGT
  GAAACTGTACATAAACCTGAATTGTCTTCAAAACCTCCCCCTTCTCCAAAAACTCTCATGAAGGCTCATA
  GTTCCACACAGCCGCCTGAGTTTGACAGTGGAGTCGAGACTTTCTCTGTTCACACAGATAAGCCTAAATA
  TCAAATAAATAATATCAGCACCATGCCTAAAGCTGTTCCTGTGAGTCCTTCAGCTGTGGAAGAAGATGAA
  GATGAGGATGGTCATACTGTAGTGGCTACAGCCCGCGGCATTTTTAACAGCAATGGCGGTGTGTTGAGTT
  CCATAGAAACTGGTGTTAGTATAATTATCCCACAAGGAGCCATTCCTGAAGGAATTGAGCAAGAAATCTA
  TTTCAAAGTCTGCAGAGACAATAGCATCCTCCCACCTTTAGATAAAGAGAAAGGTGAAACTCTGCTGAGC
  CCCCTAGTGATGTGCGGGCCCCATGGCCTCAAGTTCCTGAAGCCTGTGGAGCTACGCTTGCCACACTGTG
  ACCCTAAAACTTGGCAAAACAAGTGTCTTCCTGGAGATCCGAATTACCTTGTTGGAGCCAACTGTGTTTC
  TGTCCTGATTGACCACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206456 representing NM_001106266
  Red=Cloning site Green=Tags(s)
  
  MEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVS
  MDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSHPDPDPVSDNEDDSYDEDVHDPRSGRGALANRRGE
  KSWARDRSASRDRSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGN
  IQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEI
  QSLASDHSVRSHDRPPRRSQSRSPDQRSEPSDHSTQSPQQPSNGSLRSREEERMSKPGAVSTPVKHVDDH
  TPKAVEEVTVEKHEKQTPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDS
  VGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQK
  KKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERG
  IVPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAG
  FLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDHRSSGIIRLHTIKQIIDQDKHALLDVTPNA
  VDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGW
  YGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGA
  YTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQAIHRIDSPGLKTASQQK
  AEASSPVPYLSPETNPASSASAVKHNVNLTNVNLEEPTPAPPTSHVSQADCLGAPSPEAPHTMLRDEGVS
  LPSHVDPAKVYRKEPYPEEMMRQNHILKQPALGHPGQRLDKEPNPAYDPQLPYVEKQASRDLEQPPYRYE
  SSSYTDQFSRNYDHRLRFEDRVPTYEDQWSYYDDKQPYPTRPFDTQHPRDLDSRQHPEEASERGYFQRFE
  EPAPLPYDSRPRYEQLPRTSTLRHEEQPTSGYEVHNRYRPEAQPYAPAGPKSSEPKQYFDQYPRSYEQVP
  PPGFTSKTGHYEPLHGAAVVPPLIPSSQHKPEVLPSATKPQPPPPALTEEEEDPAMKPQSVLTRVKMFEN
  KRSASLENKKDVNDTASFKPPEVASKPPSASLVGPKPVSQTQFSEHDKTLYRLPEPQKPQAKPPEDIVRS
  NHYDPEEDEEYYRKQLSYFDRRSFESKPPAHIPAGHHSEPAKPVHSQSQPNFSSYSSKGKPETDAMDRSF
  SEKRYDPTQAMPPPPPLPSQYSQPVPPLSNSSLHIHSKAAQSEGNSVSLDFQNSYISKPDPPPSQSKPAT
  FRPPTREDPPQTFYPQKSFPDKASVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPS
  ETVHKPELSSKPPPSPKTLMKAHSSTQPPEFDSGVETFSVHTDKPKYQINNISTMPKAVPVSPSAVEEDE
  DEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLS
  PLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106266
• ORF Size: 5199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106266.1, NP_001099736.1
• RefSeq Size: 6614 bp
• RefSeq ORF: 5202 bp
• Locus ID: 292994
• Cytogenetics: 1q22
• MW: 194 kDa