Ahcyl1 (NM_001108561) Rat Tagged ORF Clone

CAT#: RR206216

Ahcyl1 (Myc-DDK-tagged ORF) - Rat S-adenosylhomocysteine hydrolase-like 1 (Ahcyl1), (10 ug)

Product Images

Circular map for RR206216

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahcyl1
• Synonyms: IRBIT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206216 representing NM_001108561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGAGTTCACCAAATTCCCTACTAAGACTGGCCGGAGATCCTTGTCTCGCTCCATCTCACAGTCCT
  CCACAGACAGCTACAGTTCAGCTGCATCCTATACAGATAGCTCTGATGATGAGGTTTCCCCCCGAGAGAA
  GCAGCAAACCAACTCAAAGGGCAGCAGCAATTTCTGTGTGAAGAACATCAAGCAGGCAGAGTTTGGACGC
  CGGGAGATTGAGATTGCAGAGCAAGACATGTCTGCTCTGATTTCACTCAGGAAACGTGCTCAGGGAGAGA
  AGCCTTTGGCTGGTGCTAAAATAGTGGGCTGTACACACATCACAGCCCAGACAGCGGTATTGATTGAGAC
  ACTTTGTGCCCTGGGGGCTCAGTGCCGCTGGTCTGCCTGCAACATCTATTCAACTCAGAATGAGGTAGCT
  GCAGCACTGGCTGAGGCTGGAGTTGCAGTGTTTGCTTGGAAGGGCGAGTCAGAAGATGATTTCTGGTGGT
  GCATTGACCGCTGTGTCAACATGGATGGGTGGCAGGCTAACATGATCCTGGATGATGGGGGAGACTTAAC
  CCACTGGGTTTATAAGAAGTATCCAAACGTGTTTAAGAAGATCCGAGGCATTGTGGAAGAGAGCGTGACT
  GGTGTTCACAGGCTGTATCAGCTCTCCAAAGCTGGGAAGCTCTGTGTTCCAGCCATGAATGTCAATGATT
  CTGTTACCAAACAGAAGTTTGATAACTTGTACTGCTGCCGAGAATCCATTTTGGATGGCCTGAAGAGGAC
  CACAGATGTGATGTTTGGTGGGAAACAGGTGGTGGTGTGTGGCTATGGTGAGGTAGGAAAGGGCTGCTGT
  GCTGCTCTCAAGGCCCTTGGAGCAATTGTCTACATCACAGAAATTGACCCCATCTGTGCTCTGCAGGCCT
  GCATGGATGGGTTCCGGGTGGTGAAGCTGAATGAAGTCATCCGGCAGGTGGACGTTGTAATAACTTGCAC
  AGGAAATAAGAATGTAGTGACCCGGGAGCACTTGGACCGAATGAAAAATAGTTGCATTGTATGTAATATG
  GGCCATTCCAACACAGAAATTGATGTGACCAGCCTCCGCACTCCAGAGCTAACATGGGAGCGCGTACGTT
  CTCAGGTGGACCATGTCATCTGGCCTGATGGCAAGCGGGTCGTCCTTCTAGCAGAGGGTCGTTTACTCAA
  TTTGAGCTGCTCCACAGTTCCTACCTTTGTCCTTTCCATCACGGCTACAACACAGGCTTTGGCACTGATA
  GAACTTTATAATGCACCAGAAGGACGCTACAAACAGGATGTGTACTTGCTTCCTAAGAAAATGGATACTA
  ATGGACGTATTACAATGACCAGTCCACACGAACCACGCAACTCTAATAGAGTATTTTTTAAGATAACTTT
  TATTTTCTTCTTATTACTTTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206216 representing NM_001108561
  Red=Cloning site Green=Tags(s)
  
  MQEFTKFPTKTGRRSLSRSISQSSTDSYSSAASYTDSSDDEVSPREKQQTNSKGSSNFCVKNIKQAEFGR
  REIEIAEQDMSALISLRKRAQGEKPLAGAKIVGCTHITAQTAVLIETLCALGAQCRWSACNIYSTQNEVA
  AALAEAGVAVFAWKGESEDDFWWCIDRCVNMDGWQANMILDDGGDLTHWVYKKYPNVFKKIRGIVEESVT
  GVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCC
  AALKALGAIVYITEIDPICALQACMDGFRVVKLNEVIRQVDVVITCTGNKNVVTREHLDRMKNSCIVCNM
  GHSNTEIDVTSLRTPELTWERVRSQVDHVIWPDGKRVVLLAEGRLLNLSCSTVPTFVLSITATTQALALI
  ELYNAPEGRYKQDVYLLPKKMDTNGRITMTSPHEPRNSNRVFFKITFIFFLLLSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108561
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108561.1, NP_001102031.1
• RefSeq Size: 3435 bp
• RefSeq ORF: 1428 bp
• Locus ID: 362013
• UniProt ID: B5DFN2
• Cytogenetics: 2q34
• MW: 52.9 kDa
• Gene Summary: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (By similarity). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates of ITPR1 to interact with CFTR and SLC26A6 mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (By similarity). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed:20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition. May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state. Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (By similarity). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (By similarity).[UniProtKB/Swiss-Prot Function]