Cdc23 (NM_001100659) Rat Tagged ORF Clone

CAT#: RR206135

Cdc23 (Myc-DDK-tagged ORF) - Rat CDC23 (cell division cycle 23, yeast, homolog) (Cdc23), (10 ug)

Product Images

Circular map for RR206135

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206135 representing NM_001100659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGAATTCTTCGGTAGTCTCGGTGGCAGCGGCCGCCACGACGGTGCCGGGCGTGTCCACTGTCG
  CGGATTTCTCAGACTTGCGGGAAATTAAAAAGCAGCTGCTGCTCATCGCGGGCCTCACTCGGGAGCGGGG
  CCTGCTGCACAGCAGCAAATGGTCTGCGGAGTTGGCCTTCTCTCTGCCCGCCTTGCCTTTGTCTGAGCTG
  CAGCCGCCGCCGCCTCTTACAGAGGAGGATGCACAGGATGTGGATGCTTACACCCTGGCCAAGGCCTACT
  TTGACGTTAAAGAGTATGACCGGGCAGCACATTTCCTGCATGGCTGTAATAGCAAGAAAGCCTATTTCCT
  GTACATGTATTCCAGATACCTGTCTGGAGAGAAGAAGAAGGATGACGAAACAGTCGATAGCCTAGGACCC
  CTAGAAAAAGGACAAGTCAAAAACGAGGCTCTCCGAGAACTGAGAGTGGAACTCAGCAGGAAGCACCAAG
  CTAGGGGACTTGATGGGTTCGGCCTTTACCTGTATGGGGTGGTGCTTCGGAAACTGGACTTAGTGAAAGA
  GGCTATTGATGTGTTTGTGGAGGCTACTCATGTTCTGCCCTTGCACTGGGGGGCCTGGTTGGAGCTGTGT
  AACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCTGACACCTGGATGAAGGAGTTCTTCT
  TGGCTCACATATACACAGAATTGCAGCTGATAGAGGAGGCCTTGCAGAAGTACCAGCATCTCATTGACGT
  GGGCTTTTCCAAGAGCTCATACATTGTTTCCCAAATCGCAGTTGCCTATCACAATATCAGAGATATCGAC
  AAAGCCCTTTCTATTTTCAATGAGCTGAGGAAACAAGATCCTTACAGGATTGAAAATATGGACACGTTCT
  CCAACCTTCTCTATGTCAGGAGCATGAAGTCTGAATTGAGTTACCTGGCACATAACCTCTGTGAAATTGA
  TAAGTACCGAGTAGAGACATGCTGTGTAATTGGCAATTATTACAGCTTACGCTCCCAGCATGAGAAAGCA
  GCGTTGTATTTCCAGAGAGCCCTGAAATTGAATCCTAGGTACCTTGGGGCCTGGACGCTGATGGGGCATG
  AGTACATGGAAATGAAAAACACATCTGCGGCTATTCAGGCTTACCGACATGCCATTGAGGTCAACAAGAG
  AGACTACAGAGCTTGGTATGGCCTTGGGCAGACCTACGAAATCCTTAAGATGCCATTTTATTGCCTTTAT
  TATTATAGACGGGCCCACCAGCTTCGGCCCAACGATTCTCGTATGCTGGTTGCCTTAGGAGAATGTTATG
  AGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCATACGCTGTGGGAGATGTGGAGAA
  AATGGCTCTGGTGAAGCTGGCCAAGCTTCATGAACAGTTGACTGAGTCAGAGCAGGCTGCCCAGTGTTAC
  ATCAAATACATCCAAGATATCTATTCCTGTGGGGAAACAGTGGAGCACTTAGAGGAGAGCACAGCCTTCC
  GCTACCTGGCCCAGTACTATTTTAAATGCAAGCTGTGGGATGAAGCTTCAACTTGTGCCCAAAAATGTTG
  TGCATTCAATGATACCCGGGAAGAAGGTAAGGCCTTGCTCCGCCAGATCCTACAGCTGAGGAACCAAGGG
  GAGACACCCACCTCTGACACGCCTGGTACCTTTTTCCTCCCTGCCTCACTGTCTGCAAACAACACTCCCA
  CACGCAGAGTTTCTCCACTCAACTTGTCTTCAGTCACACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206135 representing NM_001100659
  Red=Cloning site Green=Tags(s)
  
  MAANSSVVSVAAAATTVPGVSTVADFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLSEL
  QPPPPLTEEDAQDVDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGP
  LEKGQVKNEALRELRVELSRKHQARGLDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELC
  NLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQHLIDVGFSKSSYIVSQIAVAYHNIRDID
  KALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKA
  ALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLY
  YYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCY
  IKYIQDIYSCGETVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQG
  ETPTSDTPGTFFLPASLSANNTPTRRVSPLNLSSVTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100659
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100659.1, NP_001094129.1
• RefSeq Size: 3281 bp
• RefSeq ORF: 1794 bp
• Locus ID: 291689
• Cytogenetics: 18p12
• MW: 68.6 kDa