Ebf3 (NM_001108506) Rat Tagged ORF Clone

CAT#: RR206008

Ebf3 (Myc-DDK-tagged ORF) - Rat early B-cell factor 3 (Ebf3), (10 ug)

Product Images

Circular map for RR206008

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ebf3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206008 representing NM_001108506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATTCAGGAGAATATTCCGCGCGGGGGGACGACCATGAAGGAGGAGCCGCTGGGCAGCGGCA
  TGAACCCGGTGCGCTCGTGGATGCACACGGCAGGCGTGGTGGACGCCAACACGGCCGCCCAGAGCGGCGT
  GGGGCTGGCGCGGGCGCACTTCGAGAAGCAGCCTCCTTCCAACCTCCGGAAATCCAATTTCTTCCACTTC
  GTGCTGGCGCTCTACGACAGGCAGGGGCAGCCGGTGGAGATCGAAAGGACCGCTTTTGTGGACTTTGTGG
  AGAAAGAGAAAGAGCCGAATAACGAGAAAACCAACAACGGCATTCACTACAAGCTGCAGCTACTGTACAG
  CAATGGCGTCAGGACAGAGCAAGATCTGTATGTCCGCCTCATAGATTCAATGACCAAACAGGCCATCGTG
  TATGAAGGCCAGGACAAAAATCCGGAGATGTGCCGCGTGCTGCTGACCCATGAGATCATGTGCAGCCGGT
  GCTGTGACAAGAAGAGTTGTGGCAATAGGAACGAAACACCCTCAGACCCTGTAATCATTGACAGATTCTT
  TCTAAAGTTCTTCCTCAAGTGCAATCAGAACTGCTTGAAGAATGCGGGCAACCCTCGGGACATGCGGAGG
  TTCCAGGTCGTTGTATCGACAACCGTCAACGTGGACGGCCACGTGCTGGCCGTGTCTGACAACATGTTTG
  TGCACAACAATTCCAAACACGGGAGGCGGGCCCGCCGCCTAGACCCGTCAGAAGGTACGGCCCCTTCTTA
  TCTGGAAAATGCCACTCCATGCATCAAGGCCATCAGCCCCAGTGAAGGCTGGACCACGGGGGGCGCCACT
  GTGATCATAATCGGGGACAACTTCTTTGACGGTCTCCAAGTTGTGTTCGGAACTATGTTGGTGTGGAGTG
  AGCTGATAACCCCCCACGCCATCCGAGTGCAGACGCCGCCGCGACACATCCCTGGAGTTGTTGAAGTGAC
  CCTCTCCTACAAGTCTAAGCAGTTCTGCAAAGGCGCTCCTGGACGCTTTGTCTACACTGCCCTTAATGAG
  CCAACCATAGATTACGGCTTCCAGAGGTTGCAGAAAGTGATACCCAGACATCCAGGTGACCCCGAAAGGT
  TACCCAAGGAAGTGTTACTGAAGAGGGCGGCAGACCTTGTGGAAGCCTTGTATGGGATGCCTCATAACAA
  CCAGGAGATCATCCTGAAGAGAGCAGCCGACATTGCTGAGGCACTGTACAGCGTTCCCCGCAATCACAAC
  CAGATCCCCACCTTGGGCAACACTCCAGCACACACTGGCATGATGGGTGTGAACTCCTTCAGCAGCCAGC
  TAGCCGTTAATGTGTCAGAGACCTCACAAGCCAATGACCAAGTCGGCTACAGTCGCAATACAAGCAGCGT
  GTCCCCGCGAGGCTACGTCCCCAGCAGTACTCCCCAGCAGTCCAATTACAACACAGTCAGCACTAGCATG
  AATGGATATGGAAGTGGCGCCATGGCCAATCTAGGGGTCCCTGGCTCGCCTGGATTTCTTAATGGCTCCT
  CCGCTAACTCTCCCTACGGCATAGTGCCGTCCAGCCCCACCATGGCAGCCTCTTCGGTCACCCTCCCTTC
  AAACTGTAGCAGCACACACGGCATTTTCTCATTCTCACCTGCCAATGTCATCTCCGCAGTGAAACAGAAG
  AGCGCTTTCGCGCCCGTGGTCCGGCCCCAAGCCTCGCCTCCTCCTTCCTGCACCAGCGCCAATGGGAACG
  GACTTCAAGCTATGTCTGGGCTGGTAGTCCCGCCCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206008 representing NM_001108506
  Red=Cloning site Green=Tags(s)
  
  MFGIQENIPRGGTTMKEEPLGSGMNPVRSWMHTAGVVDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHF
  VLALYDRQGQPVEIERTAFVDFVEKEKEPNNEKTNNGIHYKLQLLYSNGVRTEQDLYVRLIDSMTKQAIV
  YEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRR
  FQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTAPSYLENATPCIKAISPSEGWTTGGAT
  VIIIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFVYTALNE
  PTIDYGFQRLQKVIPRHPGDPERLPKEVLLKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHN
  QIPTLGNTPAHTGMMGVNSFSSQLAVNVSETSQANDQVGYSRNTSSVSPRGYVPSSTPQQSNYNTVSTSM
  NGYGSGAMANLGVPGSPGFLNGSSANSPYGIVPSSPTMAASSVTLPSNCSSTHGIFSFSPANVISAVKQK
  SAFAPVVRPQASPPPSCTSANGNGLQAMSGLVVPPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108506
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108506.1, NP_001101976.1
• RefSeq Size: 4843 bp
• RefSeq ORF: 1791 bp
• Locus ID: 361668
• Cytogenetics: 1q41
• MW: 64.9 kDa