Dvl3 (NM_001107081) Rat Tagged ORF Clone

CAT#: RR204314

Dvl3 (Myc-DDK-tagged ORF) - Rat dishevelled, dsh homolog 3 (Drosophila) (Dvl3), (10 ug)

Product Images

Circular map for RR204314

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dvl3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204314 representing NM_001107081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACGATTTTGGAGTGGTGAAGGAAGAGATCTCGGATGACAATGCCAAGCTGCCCTGCTTCAATG
  GCCGGGTGGTGTCCTGGCTGGTATCGGCCGAGGGCTCACACCCAGAGCCAGCCCCTTTCTGTGCTGACAA
  CCCCTCAGAGCTGCCTCCTTCAATGGAACGCACAGGAGGCATCGGTGACTCTCGGCCCCCGTCCTTCCAC
  CCTCATGCTAGTGGGGGCAGCCAGGAGAACTTGGACAATGACACAGAGACCGACTCCTTGGTATCTGCCC
  AACGAGAGCGGCCCAGACGGAGGGATGGCCCTGAGCACGCAGCCCGCCTCAATGGAACCACAAAGGGCGA
  GCGGCGACGGGAGCCAGGTGGCTATGACAGTTCATCTACCCTCATGAGCAGCGAGCTGGAGACTACCAGT
  TTCTTTGATTCAGATGAGGATGACTCCACCAGCAGGTTCAGCAGCTCCACAGAACAGAGCAGTGCCTCAC
  GCCTAATGAGAAGACACAAGCGGCGGCGACGGAAGCAGAAGGTCTCCCGGATTGAGCGGTCCTCCTCCTT
  CAGCAGCATCACAGACTCCACTATGTCTCTCAACATCATCACAGTGACTCTCAACATGGAAAAGTATAAC
  TTCTTGGGCATCTCCATTGTGGGCCAGAGCAATGAGCGAGGTGATGGGGGCATCTACATTGGCTCCATCA
  TGAAGGGTGGGGCAGTGGCGGCTGATGGACGCATTGAGCCGGGAGATATGCTGTTGCAGGTAAATGAGAT
  CAACTTCGAGAACATGAGTAATGATGACGCAGTCAGAGTGCTTCGGGAGATCGTGCACAAACCAGGGCCC
  ATCACTCTGACTGTAGCCAAGTGCTGGGACCCAAGTCCTCGTGGCTGCTTCACATTACCCCGGAGTGAGC
  CCATCCGTCCCATTGACCCTGCAGCCTGGGTCTCCCACACTGCAGCCATGACAGGAACCTTCCCTGCCTA
  TGGCATGAGCCCCTCCTTAAGCACCATCACCTCCACCAGCTCCTCCATCACCAGCTCCATCCCCGACACA
  GAACGCCTAGATGATTTCCACCTCTCCATCCACAGTGACATGGCGGCCATCGTAAAAGCCATGGCCTCCC
  CCGAGTCAGGGCTGGAGGTCCGAGACCGCATGTGGCTCAAGATTACCATTCCCAATGCTTTCATCGGCTC
  AGATGTGGTGGACTGGCTGTATCACAATGTGGAAGGATTCACCGACCGGCGGGAGGCCCGCAAGTACGCT
  AGCAACCTGCTGAAGGCTGGATTCATCCGCCACACGGTCAACAAGATCACGTTCTCTGAGCAGTGCTACT
  ACATCTTTGGTGACCTCTGTGGCAACATGGCCAACCTATCGCTGCACGACCATGATGGCTCCAGTGGTGC
  CTCTGACCAGGACACACTGGCTCCTCTGCCGCACCCAGGGGCTGCCCCTTGGCCTATGGCTTTCCCTTAC
  CAGTACCCACCACCTCCGCACCCGTACAACCCACACCCAGGCTTCCCGGAGCTGGGCTACAGCTACGCTG
  GGGGCAGCGCCAGCAGTCAGCACAGTGAAGGTAGTAGGAGCAGTGGCTCCAACCGAAGTGGCAGCGACCG
  GCGCAAGGAGAAGGACCCAAAAGCTGGGGACTCAAAGTCAGGTGGTAGCGGCAGCGAGTCAGACCACACT
  ACACGCAGCAGTCTGCGGGGGCCACGGGAGCGGGCACCAAGCGAGCGCTCAGGCCCAGCGGCCAGCGAGC
  ACAGCCACCGCAGTCATCATTCACTGACCAGTAGCCTGCGCAGCCACCACACACACCCAAGCTATGGCCC
  ACCCGGAGTGCCCCCTCTCTATGGTCCCCCGATGCTGATGATGACCCCACCGCCTGCAGCCATGGGGCCC
  CCAGGAGCCCCTCCAGGGCGTGACCTGGCCTCGGTGCCCCCGGAACTGACGGCCAGCAGACAGTCCTTCC
  GCATGGCAATGGGAAACCCCAGTGAGTTCTTTGTGGATGTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204314 representing NM_001107081
  Red=Cloning site Green=Tags(s)
  
  MDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPEPAPFCADNPSELPPSMERTGGIGDSRPPSFH
  PHASGGSQENLDNDTETDSLVSAQRERPRRRDGPEHAARLNGTTKGERRREPGGYDSSSTLMSSELETTS
  FFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYN
  FLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGP
  ITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDT
  ERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYA
  SNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPY
  QYPPPPHPYNPHPGFPELGYSYAGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHT
  TRSSLRGPRERAPSERSGPAASEHSHRSHHSLTSSLRSHHTHPSYGPPGVPPLYGPPMLMMTPPPAAMGP
  PGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107081
• ORF Size: 2004 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107081.2, NP_001100551.1
• RefSeq Size: 2007 bp
• RefSeq ORF: 2007 bp
• Locus ID: 303811
• Cytogenetics: 11q23
• MW: 72.6 kDa