Barhl1 (NM_057109) Rat Tagged ORF Clone

CAT#: RR203962

Barhl1 (Myc-DDK-tagged ORF) - Rat BarH-like homeobox 1 (Barhl1), (10 ug)

Product Images

Circular map for RR203962

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Barhl1
• Synonyms: Barhl2; Mbh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203962 representing NM_057109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCTCCAATGGCTTTGGGATTGACTCCATTCTCTCCCACCGAGCGGGCAGCCCCGCCCTTCCCA
  AGGGGGACCCCTTGCTTGGGGACTGCCGTTCACCCCTGGAGCTGAGTCCACGCTCAGAGAGCAGCAGCGA
  CTGCTCTTCACCAGCCTCGCCCGGAAGAGACTGTCTGGAGACCAGTACCTCGCGGCCTGGTGCAGCATCT
  GGCCCAGGTTTGGACTCCCACCTGCAGCCGGGGCAGCTTTCAGCCCCAGCCCAGTCGCGAACTGTCACCT
  CCTCCTTTCTGATCAGGGACATCCTTGCTGACTGCAAACCTCTGGCGGCCTGTGCACCCTACTCTAGCAG
  TGGGCAGCCTGCAGCCCCTGAGCCTGGGGGCCGCCTTGCGGCCAAGGCCGGGGAGGACTTTCGAGACAAG
  CTGGACAAAAGTGTCAGCAGCGCTTCATCGGACTCTGAGTACAAAGTGAAGGAGGAGGGCGACCGCGAAA
  TCTCCAGCTCTCGGGACAGTCCTCCCGTGCGCCTGAAAAAGCCACGCAAAGCACGCACCGCCTTCACCGA
  CCATCAGCTGGCGCAGCTGGAGCGTAGCTTCGAGCGGCAGAAATACCTGAGTGTGCAAGACCGCATGGAG
  CTGGCCGCCTCTCTGAACCTCACCGACACGCAGGTCAAGACCTGGTACCAGAACCGCAGGACTAAATGGA
  AGCGACAGACCGCTGTGGGGCTGGAGCTGCTAGCGGAGGCAGGCAATTATTCGGCGCTCCAGAGAATGTT
  CCCGTCGCCTTATTTCTACCCGCAGAGTCTCGTTTCCAACCTGGACCCCGGCGCCGCACTCTATCTGTAC
  CGCGGACCCAGTGCGCCACCACCTGCCCTCCAGAGACCTCTGGTGCCCCGCATCCTCATCCACGGACTCC
  AGGGCGCCAGCGAGCCGCCCCCACCGCTGCCCCCGCTGCCCGGCGTCCTTCCGCGCGCTGCGCAGCCCCG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203962 representing NM_057109
  Red=Cloning site Green=Tags(s)
  
  MEGSNGFGIDSILSHRAGSPALPKGDPLLGDCRSPLELSPRSESSSDCSSPASPGRDCLETSTSRPGAAS
  GPGLDSHLQPGQLSAPAQSRTVTSSFLIRDILADCKPLAACAPYSSSGQPAAPEPGGRLAAKAGEDFRDK
  LDKSVSSASSDSEYKVKEEGDREISSSRDSPPVRLKKPRKARTAFTDHQLAQLERSFERQKYLSVQDRME
  LAASLNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYPQSLVSNLDPGAALYLY
  RGPSAPPPALQRPLVPRILIHGLQGASEPPPPLPPLPGVLPRAAQPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057109
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057109.1, NP_476450.1
• RefSeq Size: 2393 bp
• RefSeq ORF: 984 bp
• Locus ID: 117232
• UniProt ID: P63156
• Cytogenetics: 3p12
• MW: 35.1 kDa
• Gene Summary: has similarity to D. melanogastar homeotic protein BarH2 [RGD, Feb 2006]