Cdk1 (NM_019296) Rat Tagged ORF Clone

CAT#: RR202805

Cdk1 (Myc-DDK-tagged ORF) - Rat cell division cycle 2, G1 to S and G2 to M (Cdc2), (10 ug)

Product Images

Circular map for RR202805

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk1
• Synonyms: Cdc2; Cdc2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202805 representing NM_019296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTATATCAAAATAGAGAAAATCGGAGAAGGGACTTATGGTGTGGTATATAAAGGTAGACACA
  GGACTACTGGCCAGATCGTGGCCATGAAGAAAATCAGACTTGAAAGCGAGGAAGAAGGAGTGCCCAGTAC
  GGCAATCCGGGAAATCTCTCTGTTAAAAGAACTTCGACATCCAAATATAGTCAGCCTGCAGGATGTACTC
  ATGCAGGACTCCAGGCTGTATCTCATCTTCGAGTTCCTGTCCATGGATCTCAAGAAGTACCTGGACTCTA
  TCCCTCCTGGCCAGTTCATGGATTCTTCGCTCGTTAAGAGTTACTTGTACCAAATCCTCCAGGGGATTGT
  GTTTTGTCACTCCCGACGAGTTCTTCACAGAGACTTGAAACCTCAAAATCTATTGATTGATGACAAAGGA
  ACAATCAAACTGGCAGATTTCGGCCTTGCCAGAGCGTTTGGAATACCGATAAGAGTGTACACACACGAGG
  TAGTGACGCTGTGGTACCGGTCGCCAGAGGTGTTGCTGGGCTCGGCTCGCTACTCCACCCCGGTTGACAT
  CTGGAGCATAGGGACCATATTTGCAGAGCTGGCGACCAAGAAGCCGCTTTTCCACGGCGACTCAGAGATT
  GACCAGCTCTTCAGGATCTTCAGAGCTCTGGGCACCCCTAACAACGAAGTGTGGCCAGAAGTAGAGTCCC
  TGCAGGACTACAAGAACACTTTCCCCAAGTGGAAGCCGGGGAGCCTCGCGTCCCACGTCAAGAACCTGGA
  TGAAAACGGCTTGGACTTGCTCTCGAAAATGCTGGTCTATGATCCAGCCAAACGAATCTCTGGAAAAATG
  GCCCTTAAGCACCCATACTTTGACGACTTGGACAATCAGATCAAGAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202805 representing NM_019296
  Red=Cloning site Green=Tags(s)
  
  MEDYIKIEKIGEGTYGVVYKGRHRTTGQIVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVL
  MQDSRLYLIFEFLSMDLKKYLDSIPPGQFMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKG
  TIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEI
  DQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLVYDPAKRISGKM
  ALKHPYFDDLDNQIKKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019296
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019296.1, NP_062169.1
• RefSeq Size: 1184 bp
• RefSeq ORF: 894 bp
• Locus ID: 54237
• UniProt ID: P39951
• Cytogenetics: 20p11
• MW: 34.1 kDa
• Gene Summary: putative cell cycle control protein; homologous to S. pombe Cdc2 [RGD, Feb 2006]