Il33 (NM_001014166) Rat Tagged ORF Clone

CAT#: RR202106

Il33 (Myc-DDK-tagged ORF) - Rat interleukin 33 (Il33), (10 ug)

Product Images

Circular map for RR202106

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il33
• Synonyms: RGD1311155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202106 representing NM_001014166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCTAGAATGAAGTATTCGAACTCCAAGATTTCCCCGGCAAAGTGCGACAGCACATCAGGCAGAG
  CCCTGGTCCCGCCTTGCAAAATCACAAGATCCCAACAGAAGACCAAAGATATCTGCCATGTCTACTGCAT
  GAGGCTCCGTTCTGGCCTCACCATAAGAAAGGAGACCTGTTATTTTGGAAAAGAACCCGCCAAAAGATAT
  TCACTAAAATCAGGTAGCAAGCATGAAGGGAGGCTCTCTACCTGTTTACCAGATTCTAGGAAGAGATCCC
  TGCTTGGCAGTATCCAAGCGTTTGCTGCATCAGTTGACACACTGAGTATCCAAGGAACTTCACTGCTAAC
  AGAATCTTGTGCCCTGAGCACATACAACGACCAATCTGTTAGTTTTGTTTTGGAGAATGGATGTTACGTG
  ATCAATGTTGAAGATTGTGGGAAAAACCAAGAAAAAGATAAGGTGCTACTCCGTTACTACGAGTCTTCCT
  TTCCTGCACAATCAGGAGACGGTGTGGATGGGAAGAAGCTGATGGTGAACATGAGTCCTATCAAAGACAC
  AGACATCTGGCTGAATGCCAATGACAAGGACTACTCTGTGGAGCTCCAAAAGGGTGATGTCTCTCCGCCA
  GATCAGGCCTTCTTTGTTCTTCACAAAAAGTCCTCTGACTTCGTTTCATTCGAATGCAAGAATCTTCCTG
  GCACTTACATAGGAGTAAAAGACAACCAACTGGCTCTGGTAGAAGAGAATGATGAGAGCTGTAACAATAT
  CATGTTTAAGCTCTCTAAGATGCATGTTTAAGCTCTCTAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202106 representing NM_001014166
  Red=Cloning site Green=Tags(s)
  
  MRPRMKYSNSKISPAKCDSTSGRALVPPCKITRSQQKTKDICHVYCMRLRSGLTIRKETCYFGKEPAKRY
  SLKSGSKHEGRLSTCLPDSRKRSLLGSIQAFAASVDTLSIQGTSLLTESCALSTYNDQSVSFVLENGCYV
  INVEDCGKNQEKDKVLLRYYESSFPAQSGDGVDGKKLMVNMSPIKDTDIWLNANDKDYSVELQKGDVSPP
  DQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEENDESCNNIMFKLSKMHV*AL*D
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014166
• ORF Size: 792 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2471 bp
• RefSeq ORF: 795 bp
• Locus ID: 361749
• UniProt ID: Q66H70
• Cytogenetics: 1q52
• MW: 29.5 kDa
• Gene Summary: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (By similarity).[UniProtKB/Swiss-Prot Function]