Raf1 (NM_012639) Rat Tagged ORF Clone

CAT#: RR200564

Raf1 (Myc-DDK-tagged ORF) - Rat v-raf-leukemia viral oncogene 1 (Raf1), (10 ug)

Product Images

Circular map for RR200564

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Raf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200564 representing NM_012639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTCGGACTCAAAGATGCTGTGTTTGATG
  GCTCCAGTTGCATCTCCCCTACAATTGTTCAGCAGTTTGGCTATCAGCGTCGGGCCTCTGATGATGGCAA
  ACTCACGGATTCTTCTAAGACAAGCAATACTATCCGGGTTTTCTTGCCGAATAAGCAAAGGACTGTGGTC
  AATGTGCGGAATGGGATGAGCTTGCACGACTGCCTTATGAAAGCTCTGAAGGTTAGAGGCCTGCAGCCAG
  AGTGCTGTGCAGTGTTCAGACTTCTTCAGGAGCACAAAGGTAAGAAAGCACGCTTAGATTGGAACACCGA
  CGCCGCCTCTCTGATTGGAGAAGAACTGCAAGTGGATTTTTTGGATCACGTTCCACTCACAACTCACAAC
  TTTGCTCGGAAAACGTTCCTGAAGCTTGCATTCTGTGACATCTGTCAAAAGTTCCTGCTAAATGGATTTC
  GATGTCAGACTTGTGGCTACAAGTTTCATGAGCACTGTAGCACCAAAGTACCTACTATGTGTGTGGACTG
  GAGTAATATCAGACAGCTCTTGCTGTTTCCAAATTCCACTGCAAGTGACAGTGGAGTCCCAGCACCACCC
  TCTTTCACAATGCGTCGGATGCGAGAATCTGTTTCCCGGATGCCTGCTAGTTCCCAGCACAGATACTCCA
  CACCCCATGCCTTCACTTTCAACACCTCCAGCCCTTCCTCTGAAGGTTCCCTGTCCCAGAGGCAGAGGTC
  AACGTCCACTCCCAATGTCCACATGGTCAGCACCACCCTGCCTGTGGACAGCAGGATGATTGAGGATGCA
  ATTCGAAGTCACAGTGAATCAGCCTCACCTTCAGCCCTGTCCAGCAGCCCCAACAACCTGAGCCCAACAG
  GCTGGTCACAGCCCAAAACCCCTGTGCCAGCACAAAGAGAGAGGGCGCCAGGATCTGGGACCCAGGAAAA
  AAACAAAATTAGGCCTCGTGGGCAGAGAGATTCAAGTTATTACTGGGAAATAGAAGCCAGTGAGGTGATG
  CTGTCTACTCGGATTGGCTCGGGCTCCTTTGGCACTGTGTACAAGGGCAAGTGGCATGGAGATGTTGCAG
  TAAAGATCCTAAAGGTGGTTGACCCAACTCCAGAGCAACTTCAGGCCTTCAGGAACGAGGTGGCTGTTTT
  GCGCAAAACACGGCATGTTAATATCCTGCTGTTCATGGGGTACATGACAAAGGACAACCTGGCGATTGTG
  ACCCAGTGGTGTGAAGGCAGCAGTCTCTACAAACACCTGCATGTCCAGGAGACCAAATTCCAGATGTTCC
  AGCTAATTGACATTGCCCGGCAGACAGCTCAGGGAATGGACTATTTACATGCAAAGAACATCATCCACAG
  AGACATGAAATCCAACAATATATTTCTCCATGAAGGCCTCACGGTGAAAATCGGAGATTTTGGTTTGGCA
  ACAGTGAAGTCGCGCTGGAGTGGTTCTCAGCAGGTTGAACAGCCCACTGGCTCTGTGCTGTGGATGGCCC
  CAGAAGTAATCCGAATGCAGGATAACAACCCGTTCAGCTTCCAGTCCGATGTCTACTCCTATGGCATTGT
  GCTGTATGAGCTGATGACTGGGGAGCTTCCCTACTCCCACATCAACAACCGAGACCAGATCATCTTCATG
  GTGGGCCGTGGGTACGCCTCCCCAGATCTTAGCAGGCTCTACAAGAACTGCCCCAAGGCAATGAAGAGGT
  TGGTGGCTGACTGTGTGAAGAAAGTCAAAGAAGAAAGGCCTTTGTTTCCTCAGATCCTGTCTTCCATTGA
  GCTGCTTCAGCACTCTCTGCCGAAAATCAACAGGAGCGCCTCTGAGCCTTCCCTGCATCGGGCAGCTCAC
  ACTGAGGACATCAATGCTTGTACGCTGACCACATCCCCAAGGCTACCAGTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200564 representing NM_012639
  Red=Cloning site Green=Tags(s)
  
  MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVV
  NVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHN
  FARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTASDSGVPAPP
  SFTMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDA
  IRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVM
  LSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIV
  TQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLA
  TVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFM
  VGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAH
  TEDINACTLTTSPRLPVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012639
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012639.2, NP_036771.1
• RefSeq Size: 2970 bp
• RefSeq ORF: 1947 bp
• Locus ID: 24703
• UniProt ID: P11345
• Cytogenetics: 4q42
• MW: 72.9 kDa
• Gene Summary: acts as a mitogenic protein kinase; mutant forms may play a role in transformation [RGD, Feb 2006]