Raf1 (NM_012639) Rat Untagged Clone

CAT#: RN200564

Raf1 (untagged ORF) - Rat v-raf-leukemia viral oncogene 1 (Raf1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Raf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200564 representing NM_012639
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACATACAGGGAGCTTGGAAGACGATCAGCAATGGTTTCGGACTCAAAGATGCTGTGTTTGATG
  GCTCCAGTTGCATCTCCCCTACAATTGTTCAGCAGTTTGGCTATCAGCGTCGGGCCTCTGATGATGGCAA
  ACTCACGGATTCTTCTAAGACAAGCAATACTATCCGGGTTTTCTTGCCGAATAAGCAAAGGACTGTGGTC
  AATGTGCGGAATGGGATGAGCTTGCACGACTGCCTTATGAAAGCTCTGAAGGTTAGAGGCCTGCAGCCAG
  AGTGCTGTGCAGTGTTCAGACTTCTTCAGGAGCACAAAGGTAAGAAAGCACGCTTAGATTGGAACACCGA
  CGCCGCCTCTCTGATTGGAGAAGAACTGCAAGTGGATTTTTTGGATCACGTTCCACTCACAACTCACAAC
  TTTGCTCGGAAAACGTTCCTGAAGCTTGCATTCTGTGACATCTGTCAAAAGTTCCTGCTAAATGGATTTC
  GATGTCAGACTTGTGGCTACAAGTTTCATGAGCACTGTAGCACCAAAGTACCTACTATGTGTGTGGACTG
  GAGTAATATCAGACAGCTCTTGCTGTTTCCAAATTCCACTGCAAGTGACAGTGGAGTCCCAGCACCACCC
  TCTTTCACAATGCGTCGGATGCGAGAATCTGTTTCCCGGATGCCTGCTAGTTCCCAGCACAGATACTCCA
  CACCCCATGCCTTCACTTTCAACACCTCCAGCCCTTCCTCTGAAGGTTCCCTGTCCCAGAGGCAGAGGTC
  AACGTCCACTCCCAATGTCCACATGGTCAGCACCACCCTGCCTGTGGACAGCAGGATGATTGAGGATGCA
  ATTCGAAGTCACAGTGAATCAGCCTCACCTTCAGCCCTGTCCAGCAGCCCCAACAACCTGAGCCCAACAG
  GCTGGTCACAGCCCAAAACCCCTGTGCCAGCACAAAGAGAGAGGGCGCCAGGATCTGGGACCCAGGAAAA
  AAACAAAATTAGGCCTCGTGGGCAGAGAGATTCAAGTTATTACTGGGAAATAGAAGCCAGTGAGGTGATG
  CTGTCTACTCGGATTGGCTCGGGCTCCTTTGGCACTGTGTACAAGGGCAAGTGGCATGGAGATGTTGCAG
  TAAAGATCCTAAAGGTGGTTGACCCAACTCCAGAGCAACTTCAGGCCTTCAGGAACGAGGTGGCTGTTTT
  GCGCAAAACACGGCATGTTAATATCCTGCTGTTCATGGGGTACATGACAAAGGACAACCTGGCGATTGTG
  ACCCAGTGGTGTGAAGGCAGCAGTCTCTACAAACACCTGCATGTCCAGGAGACCAAATTCCAGATGTTCC
  AGCTAATTGACATTGCCCGGCAGACAGCTCAGGGAATGGACTATTTACATGCAAAGAACATCATCCACAG
  AGACATGAAATCCAACAATATATTTCTCCATGAAGGCCTCACGGTGAAAATCGGAGATTTTGGTTTGGCA
  ACAGTGAAGTCGCGCTGGAGTGGTTCTCAGCAGGTTGAACAGCCCACTGGCTCTGTGCTGTGGATGGCCC
  CAGAAGTAATCCGAATGCAGGATAACAACCCGTTCAGCTTCCAGTCCGATGTCTACTCCTATGGCATTGT
  GCTGTATGAGCTGATGACTGGGGAGCTTCCCTACTCCCACATCAACAACCGAGACCAGATCATCTTCATG
  GTGGGCCGTGGGTACGCCTCCCCAGATCTTAGCAGGCTCTACAAGAACTGCCCCAAGGCAATGAAGAGGT
  TGGTGGCTGACTGTGTGAAGAAAGTCAAAGAAGAAAGGCCTTTGTTTCCTCAGATCCTGTCTTCCATTGA
  GCTGCTTCAGCACTCTCTGCCGAAAATCAACAGGAGCGCCTCTGAGCCTTCCCTGCATCGGGCAGCTCAC
  ACTGAGGACATCAATGCTTGTACGCTGACCACATCCCCAAGGCTACCAGTCTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012639
• Insert Size: 1947 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012639.2, NP_036771.1
• RefSeq Size: 2970 bp
• RefSeq ORF: 1947 bp
• Locus ID: 24703
• UniProt ID: P11345
• Cytogenetics: 4q42
• Gene Summary: acts as a mitogenic protein kinase; mutant forms may play a role in transformation [RGD, Feb 2006]