Atp2a2 (NM_001110139) Rat Tagged ORF Clone

CAT#: RR200367

Atp2a2 (Myc-DDK-tagged ORF) - Rat ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 (Atp2a2), transcript variant 2, (10 ug)

Product Images

Circular map for RR200367

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2a2
• Synonyms: Serca2; SercaII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200367 representing NM_001110139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGCTCACACAAAGACCGTGGAGGAGGTGCTGGGCCACTTCGGCGTCAACGAGAGCACGGGGC
  TGAGCCTGGAGCAGGTCAAGAAGCTCAAGGAGAGATGGGGCTCCAACGAATTGCCGGCTGAAGAAGGAAA
  GACCTTGCTGGAACTTGTGATCGAGCAGTTTGAAGACTTACTAGTTAGAATTTTATTGCTGGCAGCATGT
  ATATCTTTCGTTTTGGCTTGGTTCGAAGAAGGTGAAGAAACGATTACAGCCTTTGTAGAACCTTTTGTAA
  TTCTGCTTATATTGGTAGCCAATGCAATTGTGGGTGTATGGCAGGAGAGAAACGCTGAAAATGCAATAGA
  AGCTCTTAAGGAGTATGAACCTGAAATGGGCAAGGTGTATCGACAGGACAGAAAGAGTGTGCAGCGGATT
  AAAGCGAAAGATATAGTTCCTGGGGATATAGTGGAAATTGCTGTTGGTGACAAAGTTCCGGCTGACATTA
  GATTGACATCCATCAAGTCTACAACTCTGAGAGTTGACCAGTCGATTCTTACAGGTGAATCTGTCTCGGT
  CATCAAGCATACTGACCCTGTCCCTGACCCACGAGCTGTTAATCAAGACAAAAAGAACATGCTCTTTTCT
  GGCACAAACATCGCTGCTGGCAAAGCTATGGGAGTGGTGGTGGCGACTGGAGTCAATACTGAGATCGGCA
  AGATCCGGGATGAAATGGTTGCAACAGAACAGGAGAGAACACCCCTACAGCAGAAGCTGGACGAGTTTGG
  GGAACAGCTTTCCAAAGTTATCTCCCTCATTTGCATTGCAGTCTGGATCATCAACATCGGGCATTTCAAT
  GACCCAGTTCATGGTGGCTCTTGGATCAGAGGTGCCATCTACTACTTTAAGATTGCAGTGGCCCTGGCTG
  TTGCTGCCATCCCTGAGGGTCTGCCTGCTGTCATCACCACCTGCTTGGCTCTTGGAACTCGAAGGATGGC
  AAAGAAAAATGCTATTGTTCGAAGTCTGCCTTCTGTGGAAACCCTTGGTTGTACTTCTGTTATCTGCTCA
  GACAAGACCGGCACACTTACCACAAACCAGATGTCCGTCTGCAGGATGTTCATTCTGGACAAAGTAGAAG
  GTGATACTTGTTCCCTTAATGAGTTTACCATAACTGGATCAACCTATGCACCCATTGGAGAAGTGCAAAA
  AGATGACAAGCCAGTGAAATGCCATCAGTATGACGGGCTTGTAGAGTTAGCAACGATCTGTGCTCTGTGT
  AATGACTCTGCTTTGGATTACAATGAGGCGAAGGGTGTGTATGAAAAAGTTGGAGAAGCCACAGAGACTG
  CTCTCACGTGCCTGGTAGAGAAGATGAATGTATTCGACACGGAGCTGAAGGGACTTTCTAAAATAGAACG
  CGCCAACGCCTGCAACTCGGTCATAAAACAGCTCATGAAGAAGGAATTCACGCTAGAGTTTTCACGTGAT
  AGAAAATCAATGTCCGTCTACTGTACACCAAACAAACCGAGCCGGACGTCCATGAGCAAGATGTTTGTGA
  AGGGTGCTCCAGAAGGTGTCATCGACAGGTGCACCCACATCCGAGTTGGAAGTACCAAGGTCCCCATGAC
  GCCTGGTGTTAAACAGAAGATTATGTCTGTCATTCGGGAGTGGGGCAGTGGCAGCGACACACTGCGGTGC
  CTGGCTCTGGCCACTCATGACAACCCGCTGAGGAGAGAGGAGATGCACCTGGAAGATTCTGCGAACTTCA
  TCAAATATGAGACCAATCTGACTTTCGTTGGCTGTGTGGGCATGCTGGACCCTCCCAGGATTGAAGTGGC
  CTCTTCTGTGAAGCTGTGCCGGCAAGCGGGCATCCGAGTCATCATGATCACTGGGGATAACAAAGGCACT
  GCTGTGGCCATCTGTCGCCGCATTGGCATCTTTGGGCAGGATGAGGATGTGACATCAAAGGCTTTTACAG
  GGCGAGAATTTGATGAATTAAGCCCCTCAGCCCAGAGAGACGCCTGCTTAAATGCCCGTTGTTTTGCTCG
  AGTTGAACCTTCCCACAAGTCTAAGATCGTTGAGTTCCTGCAGTCCTTTGATGAGATCACAGCTATGACT
  GGTGATGGTGTGAACGACGCGCCCGCTCTGAAGAAGTCGGAAATCGGGATTGCCATGGGCTCAGGGACTG
  CAGTGGCTAAGACGGCCTCTGAGATGGTGCTGGCCGACGACAACTTCTCCACCATCGTGGCCGCTGTTGA
  GGAGGGGCGCGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAACGTGGGGGAG
  GTGGTCTGTATCTTCCTGACGGCAGCCCTTGGGTTTCCTGAAGCTTTGATTCCTGTCCAGTTACTCTGGG
  TCAATCTGGTGACGGATGGTCTGCCTGCCACTGCGCTGGGGTTCAATCCTCCAGATCTGGACATCATGAA
  CAAACCCCCACGGAACCCAAAAGAACCGCTGATCAGCGGGTGGCTCTTTTTCCGTTACCTGGCTATTGGC
  TGTTATGTTGGCGCTGCCACCGTGGGTGCTGCTGCGTGGTGGTTCATCGCTGCTGACGGTGGTCCGAGAG
  TCTCCTTCTACCAGCTGAGTCATTTCCTGCAGTGTAAGGAGGACAACCCAGACTTCGAAGGAGTGGATTG
  TGCAATCTTTGAGTCCCCGTATCCGATGACAATGGCACTTTCTGTTCTAGTCACCATAGAGATGTGCAAT
  GCCCTCAACAGCTTGTCTGAAAACCAGTCCCTGCTGAGGATGCCCCCCTGGGAGAACATCTGGCTCGTGG
  GCTCCATCTGCTTGTCCATGTCCCTTCACTTCTTGATCCTCTACGTGGAACCTTTGCCACTCATTTTCCA
  GATCACACCGCTGAATCTGACCCAGTGGCTGATGGTGCTGAAAATCTCCCTGCCTGTGATCCTCATGGAC
  GAGACGCTCAAGTTTGTGGCCCGAAACTACCTGGAGCCTGCAATACTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200367 representing NM_001110139
  Red=Cloning site Green=Tags(s)
  
  MENAHTKTVEEVLGHFGVNESTGLSLEQVKKLKERWGSNELPAEEGKTLLELVIEQFEDLLVRILLLAAC
  ISFVLAWFEEGEETITAFVEPFVILLILVANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRI
  KAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFS
  GTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKLDEFGEQLSKVISLICIAVWIINIGHFN
  DPVHGGSWIRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCRMFILDKVEGDTCSLNEFTITGSTYAPIGEVQKDDKPVKCHQYDGLVELATICALC
  NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRD
  RKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTPGVKQKIMSVIREWGSGSDTLRC
  LALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGT
  AVAICRRIGIFGQDEDVTSKAFTGREFDELSPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMT
  GDGVNDAPALKKSEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGE
  VVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMNKPPRNPKEPLISGWLFFRYLAIG
  CYVGAATVGAAAWWFIAADGGPRVSFYQLSHFLQCKEDNPDFEGVDCAIFESPYPMTMALSVLVTIEMCN
  ALNSLSENQSLLRMPPWENIWLVGSICLSMSLHFLILYVEPLPLIFQITPLNLTQWLMVLKISLPVILMD
  ETLKFVARNYLEPAILE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110139
• ORF Size: 2991 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110139.2, NP_001103609.1
• RefSeq Size: 4303 bp
• RefSeq ORF: 2994 bp
• Locus ID: 29693
• UniProt ID: P11507
• Cytogenetics: 12q16
• MW: 109.7 kDa
• Gene Summary: sarco(endo)plasmic reticulum Ca(2+) ATPase; involved in calcium transport and cycling in the heart and in calcium sequestering in enamel forming cells [RGD, Feb 2006]