Gls2 (NM_001270786) Rat Untagged Clone

CAT#: RN216984

Gls2 (untagged) - Rat glutaminase 2 (liver, mitochondrial) (Gls2), transcript variant 1

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gls2
• Synonyms: Ga
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216984 representing NM_001270786
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCCATGAGGGCTCTGCAGAACGCGCTGAGCCGCGCGGGCAGCCACGGCCAGAGGGGAGGCTGGG
  GTCACCCGAGCCGGGGCCCGCTCCTGGGCGGGGGTGTCCGGTACTACTTCGGTGAGGCCGCGGCGCAGGG
  CAGGGGGACGCCGCACAGCCACCAGCCGCAGCACTCGGACCATGATGCCTCGAACAGTGGCATGTTGCCT
  CGACTTGGTGACCTGCTTTTCTACACCATTGCAGAGGGGCAGGAACGCATCCCCATCCATAAGTTCACCA
  CGGCCCTGAAGGCCACTGGACTGCAGACGTCAGACCCACGGCTCCAGGACTGCATGAGCAAGATGCAGCG
  CATGGTCCAAGAGTCCAGCAGTGGTGGCCTCTTGGACCGAGAACTCTTCCAAAAGTGTGTGAGCAGCAAC
  ATTGTGCTCCTGACTCAGGCATTCCGGAAGAAGTTTGTCATTCCTGACTTTGAGGAGTTCACAGGCCATG
  TGGATCGTATATTTGAGGATGCCAAAGAGCTCACTGGAGGCAAAGTGGCAGCCTACATCCCTCACCTGGC
  CAAGTCAAACCCAGATCTTTGGGGCGTCTCCCTGTGCACTGTGGATGGGCAGCGACACTCTGTGGGCCAC
  ACGAAGATCCCTTTCTGCCTACAGTCCTGTGTCAAGCCACTCACTTACGCCATCTCCGTGAGCACCTTAG
  GCACCGACTACGTGCACAAGTTCGTGGGCAAGGAACCCAGTGGTCTGCGCTATAACAAACTCTCCCTCAA
  CGAGGAAGGAATCCCTCATAACCCCATGGTCAATGCTGGTGCTATTGTGGTCAGCTCCCTGATCAAGATG
  GACTGTAACAAAGCAGAGAAGTTTGACTTTGTGTTACAGTATCTGAACAAGATGGCTGGGAATGAATTCA
  TGGGGTTCAGCAATGCCACATTCCAGTCAGAGAAGGAGACTGGGGATCGGAATTACGCCATTGGCTATTA
  CCTCAAGGAGAAGAAGTGCTTCCCTAAGGGAGTGGACATGATGGCTGCCCTTGATCTCTATTTCCAGCTG
  TGCTCTGTGGAGGTTACCTGTGAGTCAGGCAGTGTCATGGCGGCCACTCTTGCCAATGGCGGCATCTGCC
  CCATCACAGGGGAGAGCGTGCTGAGTGCGGAAGCCGTGCGCAACACCCTCAGCCTCATGCACTCCTGTGG
  CATGTATGACTTCTCGGGCCAGTTTGCCTTCCACGTGGGCCTGCCAGCCAAGTCAGCTGTGTCGGGAGCC
  ATCCTCCTGGTTGTACCCAATGTCATGGGGATGATGTGTCTGTCACCCCCGTTAGACAAGCTGGGGAACA
  GCCACAGGGGCATCAGCTTCTGCCAGAAGTTGGTGTCTCTGTTTAACTTCCACAACTACGACAACCTGCG
  GCACTGTGCTCGGAAGTTAGACCCACGGAGGGAAGGGGGGGAAGTTCGGAACAAGACCGTGGTGAACCTG
  TTATTTGCTGCATATAGTGGAGATGTCTCAGCTCTTCGAAGGTTTGCCTTGTCTGCCGTGGATATGGAGC
  AGAAGGACTATGATTCCCGCACAGCCCTACATGTGGCGGCAGCGGAAGGACACATTGACGTTGTCAAGTT
  TCTGATCGAGGCTTGCAAAGTGAATCCTTTTGTCAAGGACAGGTGGGGCAACATTCCCCTGGATGATGCC
  GTGCAGTTCAATCACCTGGAGGTGGTCAAACTGCTTCAGGATTACCATGACTCCTACATGCTGTCTGAGA
  CTCAAGCTGAGGTAGCAGCTGAGACTCTGTCAAAAGAGAACTTAGAGAGCATGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001270786
• Insert Size: 1809 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270786.1, NP_001257715.1
• RefSeq Size: 2552 bp
• RefSeq ORF: 1809 bp
• Locus ID: 192268
• UniProt ID: P28492
• Cytogenetics: 7q11
• Gene Summary: first enzyme of glutamine catabolism in liver [RGD, Feb 2006]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.