Hif1a (NM_024359) Rat Untagged Clone

CAT#: RN210999

Hif1a (untagged ORF) - Rat hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (Hif1a), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hif1a
• Synonyms: MOP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210999 representing NM_024359
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAATAGGATGAGTTCCGAACGTCGAAAAGAAAAGTCTA
  GGGATGCAGCACGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACT
  TCCCCACAACGTGAGCTCCCATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTACTTACGTGTG
  AGGAAACTTCTAGGTGCTGGTGATCTTGACATTGAAGATGAAATGAAAGCACAGATGAACTGCTTTTATC
  TGAAAGCCCTGGATGGCTTTGTTATGGTGCTAACAGATGATGGTGACATGATTTACATTTCTGATAACGT
  GAACAAATACATGGGGTTGACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACCCATCCATGT
  GACCATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAGGGGAAAGAACAAAACA
  CGCAGCGAAGCTTTTTTCTCAGAATGAAATGTACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTC
  AGCAACGTGGAAGGTGCTGCACTGCACAGGCCACATTCATGTGTATGATACCAGCAGTAACCAGCCGCAG
  TGTGGCTACAAGAAACCGCCTATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCATCAAACA
  TTGAAATTCCTTTAGACAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGA
  TGAAAGGATTACTGAGTTGATGGGTTATGAGCCAGAAGAACTTTTGGGCCGTTCAATTTATGAATATTAT
  CATGCTTTGGACTCTGATCATCTGACCAAAACTCATCATGACATGTTTACTAAAGGACAAGTCACCACAG
  GACAGTACAGGATGCTTGCAAAAAGAGGTGGATATGTCTGGGTTGAGACTCAAGCAACTGTTATATATAA
  TACGAAGAACTCTCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGAC
  TTGATTTTCTCCCTTCAACAAACAGAATCTGTCCTCAAACCAGTTGAATCTTCAGATATGAAAATGACCC
  AGCTGTTCACTAAAGTGGAATCTGAGGACACGAGCTGCCTCTTCGACAAGCTTAAGAAAGAGCCCGATGC
  CCTGACTCTGCTAGCTCCAGCGGCTGGGGACACGATCATATCACTGGACTTCGGCAGCGATGACACGGAA
  ACTGAAGACCAACAACTTGAAGATGTCCCGTTGTACAATGATGTAATGTTCCCCTCTTCTAATGAGAAAT
  TAAATATAAATCTGGCAATGTCTCCATTACCTGCCTCTGAAACTCCAAAGCCACTTCGAAGTAGTGCTGA
  TCCTGCACTGAATCAAGAGGTTGCATTGAAGTTAGAGTCAAGCCCAGAGTCACTGGGACTTTCTTTTACC
  ATGCCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGCTCACCTGAGCCTA
  ACAGTCCCAGTGAGTACTGCTTTGATGTGGACAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGA
  AAAACTGTTTGCTGAAGACACAGAAGCGAAGAATCCATTTTCAGCTCAGGACACTGATTTAGACTTGGAA
  ATGCTGGCTCCCTATATCCCAATGGATGATGATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAG
  AGAGCAATTCTCCAAGCCCTCCGAGTGTGAGCACAGTTACAGGATTCCAGCAGACCCAGTTACAGAAACC
  TACCATCACTGTCACTGCCACCGCAACTGCCACCACTGATGAATCAAAAGCAGTGACGAAGGACAATATA
  GAAGACATTAAAATACTGATTGCATCTCCACCTTCTACCCAAGTACCTCAAGAAATGACCACTGCTAAGG
  CATCAGCATACAGTGGTACTCACAGTCGGACAGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAAAA
  AACAGACAAAGCTCATCCAAGGAGCCTTAACCTATCTGTCACTTTGAATCAAAGAAATACTGTTCCTGAA
  GAAGAATTAAACCCAAAGACAATAGCTTTGCAGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCT
  CCCTTTTTCAAGCAGCAGGAATTGGAACGTTACTGCAGCAACCAGGTGACCGTGCCCCTACTATGTCGCT
  TTCTTGGAAACGAGTGAAAGGATACATATCTAGTGAACAGGATGGAATGGAGCAGAAGACAATTTTTTTA
  ATACCCTCTGATTTAGCATGTAGACTGCTGGGGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCA
  GTTACGATTGTGAAGTTAATGCTCCCATACAAGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAG
  AGCTTTGGATCAAGTTAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024359
• Insert Size: 2472 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024359.1, NP_077335.1
• RefSeq Size: 3718 bp
• RefSeq ORF: 2472 bp
• Locus ID: 29560
• UniProt ID: O35800
• Cytogenetics: 6q24
• Gene Summary: regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006]