Akt3 (NM_031575) Rat Untagged Clone

CAT#: RN208375

Akt3 (untagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (Akt3), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Akt3
• Synonyms: Pkbg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN208375 representing NM_031575
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGATGTTACCATCGTTAAAGAAGACTGGGTTCAGAAGAGGGGAGAATATATAAAAAATTGGAGGC
  CAAGATACTTCCTTTTGAAGACAGACGGCTCATTCATAGGCTATAAAGAGAAACCTCAAGATGTGGACTT
  ACCTTATCCCCTCAACAACTTCTCAGTGGCAAAATGTCAATTAATGAAAACAGAACGACCAAAGCCAAAT
  ACATTTATTATCAGATGTCTTCAGTGGACCACTGTTATAGAGAGAACATTTCATGTAGATACTCCAGAGG
  AAAGAGAAGAATGGACAGAAGCTATCCAAGCTGTAGCCGATCGACTGCAGAGGCAAGAAGAGGAGAGGAT
  GAATTGTAGTCCAACGTCACAGATTGATAATATTGGAGAGGAAGAGATGGATGCATCTACAACCCATCAT
  AAAAGAAAGACAATGAATGATTTTGACTATTTGAAACTGCTAGGTAAAGGCACTTTTGGGAAGGTTATTT
  TGGTTCGAGAGAAGGCAAGTGGAAAATACTACGCTATGAAGATTCTGAAGAAAGAAGTCATTATCGCAAA
  GGATGAAGTGGCACATACTCTGACTGAGAGCAGAGTTCTAAAGAACACCAGACATCCATTTCTAACATCC
  TTGAAATATTCCTTCCAGACAAAAGACCGTTTGTGTTTTGTGATGGAATATGTTAATGGGGGAGAGCTGT
  TTTTCCATTTGTCGAGAGAGCGGGTGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTC
  TGCTTTGGACTATCTACATTCTGGAAAGATTGTGTACCGAGATCTCAAGTTGGAGAATTTGATGCTAGAT
  AAGGATGGCCATATAAAAATTACGGATTTTGGACTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGA
  AGACATTCTGTGGTACACCAGAGTATCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCTGT
  GGACTGGTGGGGCCTAGGTGTTGTCATGTATGAAATGATGTGTGGGAGGTTGCCTTTCTACAACCAGGAT
  CATGAGAAACTCTTTGAACTAATACTCATGGAAGACATCAAATTCCCCCGAACACTCTCTTCAGATGCAA
  AGTCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATCCAAA
  AGAGATAATGAGGCACAGTTTCTTTTCTGGAGTAAACTGGCAGGACGTATATGACAAAAAGCTTGTACCT
  CCTTTTAAGCCTCAAGTAACATCTGAGACAGACACCAGATATTTCGATGAAGAATTTACAGCTCAGACTA
  TTACAATAACACCACCTGAAAAGTGTCCACTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031575
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031575.1, NP_113763.1
• RefSeq Size: 1548 bp
• RefSeq ORF: 1365 bp
• Locus ID: 29414
• UniProt ID: Q63484
• Cytogenetics: 13q25
• Gene Summary: kinase that associates with both protein kinase C subspecies and beta gamma subunits of G proteins [RGD, Feb 2006]