Cckbr (NM_013165) Rat Untagged Clone

CAT#: RN206267

Cckbr (untagged ORF) - Rat cholecystokinin B receptor (Cckbr), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Cckbr
• Synonyms: Cck2r; Cholrec
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206267 representing NM_013165
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTCAAGCTGAACCGCAGCGTGCAGGGACCAGGACCCGGGTCGGGGTCTTCTTTGTGCCGCC
  CGGGTGTCTCCCTTCTCAACAGCAGTAGTGCCGGGAACCTCAGCTGTGACCCCCCTCGTATCCGCGGAAC
  CGGGACCAGAGAATTGGAGATGGCGATTAGAATCACCCTTTATGCAGTGATCTTTCTGATGAGTGTTGGC
  GGAAACGTGCTCATCATCGTGGTCCTGGGACTGAGCCGACGCCTAAGAACGGTCACCAACGCCTTCCTGC
  TCTCCCTGGCAGTCAGCGACCTCCTGCTGGCCGTGGCTTGCATGCCCTTCACACTCCTGCCCAACCTCAT
  GGGCACATTCATCTTCGGCACAGTCATCTGCAAGGCCATTTCCTACCTCATGGGGGTATCAGTGAGTGTA
  TCCACTCTAAATCTCGTGGCCATAGCCCTGGAGCGATACAGCGCCATCTGCCGACCACTGCAAGCACGAG
  TATGGCAAACACGCTCCCACGCAGCTCGGGTGATCTTAGCCACGTGGCTGCTGTCTGGACTGCTTATGGT
  ACCCTACCCTGTGTACACCATGGTACAGCCAGTGGGACCTCGAGTGCTGCAGTGCATGCATCGCTGGCCC
  AGTGCACGTGTCCAACAAACCTGGTCCGTGCTACTGCTACTGCTTTTGTTCTTCATCCCGGGTGTGGTTA
  TTGCGGTGGCCTATGGACTCATCTCCCGCGAACTCTACCTAGGACTCCACTTTGATGGTGAAAATGACAG
  CGAGACCCAAAGCCGGGCCCGAAACCAAGGGGGCCTGCCGGGTGGGGCAGCACCAGGGCCTGTCCACCAG
  AACGGGGGCTGCCGGCCTGTAACCAGCGTAGCTGGGGAAGACAGTGATGGCTGCTGTGTGCAACTTCCGC
  GTTCCCGACTGGAGATGACAACGCTAACCACACCCACTCCTGGGCCAGTCCCTGGCCCTCGGCCCAACCA
  GGCCAAGCTGCTGGCTAAGAAGCGGGTGGTGCGAATGCTGCTAGTGATTGTTTTGCTTTTCTTCCTGTGT
  TGGCTGCCAGTGTACAGCGTCAACACGTGGCGCGCCTTCGATGGCCCAGGCGCACAACGAGCACTCTCAG
  GGGCCCCTATCTCTTTCATCCACTTGCTGAGCTACGTCTCTGCTTGTGTCAACCCCCTGGTCTACTGTTT
  CATGCACCGCCGCTTCCGCCAGGCCTGCCTGGACACATGTGCCCGCTGTTGCCCACGCCCTCCACGAGCT
  CGCCCACAGCCTCTTCCAGATGAGGATCCTCCTACCCCCTCCATCGCTTCGCTGTCCAGGCTAAGCTATA
  CCACCATCAGCACACTGGGGCCTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013165
• Insert Size: 1359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013165.2, NP_037297.1
• RefSeq Size: 2152 bp
• RefSeq ORF: 1359 bp
• Locus ID: 25706
• UniProt ID: P30553
• Cytogenetics: 1q32
• Gene Summary: Cholecystokinin was one of the first gastrointestinal peptides discovered in the mammalian brain. Cholecystokinin receptors are members of the G protein-coupled receptor superfamily, stimulating phosphatidylinositol turnover and intracellular calcium mobilization. This gene encodes a cholecystokinin receptor of the B subtype. These receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. [provided by RefSeq, Jul 2008]