Hsd3b1 (NM_001007719) Rat Untagged Clone

CAT#: RN205099

Hsd3b1 (untagged ORF) - Rat hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Hsd3b1
• Synonyms: MGC105549
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205099 representing NM_001007719
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGATGGAGCTGCCTGGTGACAGGAGCAGGAGGGTTTGTGGGCCAGAGGATCATCCGGATGTTGG
  TGCAGGAGAAAGAACTGCAGGAGGTCAGGGCTCTGGACAAAGTCTTCAGACCAGAAACCAAGGAGGAATT
  CTCTAAGCTGCAGACAAAGGCCAAGGTGACAATGTTAGAAGGAGATATTCTGGATGCCCAGTACCTGAGG
  AGAGCGTGCCAGGGCATCTCTGTTGTCATCCACACCGCTGCTGTCATTGATGTCTCACATGTCCTACCCA
  GGCAGACCATCCTAGATGTCAATCTGAAAGGTACCCAGAACATATTGGAGGCCTGCGTCGAAGCCAGTGT
  GCCAGCCTTCATCTACTGCAGCACAGTTGACGTTGCAGGGCCCAACTCCTACAAGAAGATCATCCTGAAT
  GGCCATGAGGAAGAGCATCATGAAAGCACATGGTCAGATGCATACCCATACAGCAAAAGGATGGCCGAGA
  AGGCAGTGCTGGCAGCCAATGGGAGCATCCTGAAAAATGGTGGCACACTGCATACTTGTGCCTTAAGGCC
  CATGTACATTTATGGGGAGAGAAGTCCATTCCTTTCTGTCATGATACTTGCGGCCCTCAAAAATAAGGGT
  ATTCTGAATGTTACTGGCAAATTCTCCATAGCCAATCCAGTGTATGTAGGCAATGTGGCCTGGGCACATA
  TTCTGGCTGCCAGGGGCCTTCGAGACCCCAAGAAGTCACAAAATGTCCAAGGACAGTTCTACTACATCTC
  AGATGACACCCCTCACCAAAGCTATGATGATTTAAATTGCACCCTGAGCAAGGAATGGGGCCTCCGCCTT
  GATTCCAGCTGGAGCCTTCCTCTGCCCCTGCTCTACTGGCTTGCCTTCCTGCTGGAAACTGTGAGCTTCC
  TGCTGCGTCCATTTTACAACTATAGGCCACCCTTTAACTGCCACTTGGTCACACTGTCAAATAGCAAGTT
  CACTTTCTCCTACAAGAAAGCTCAGAGAGATCTGGGCTATGTGCCACTTGTCAGCTGGGAGGAAGCCAAG
  CAGAAAACCTCGGAGTGGATCGGGACACTAGTGGAGCAGCACAGGGAGACACTGGACACAAAGTCTCAGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007719
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007719.3, NP_001007720.3
• RefSeq Size: 1640 bp
• RefSeq ORF: 1122 bp
• Locus ID: 360348
• UniProt ID: P22071
• Cytogenetics: 2q34
• Gene Summary: A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone (PubMed:1537836, PubMed:1985917). Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1537836). Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).[UniProtKB/Swiss-Prot Function]