GRK4 (NM_005307) Human Tagged ORF Clone

CAT#: RG238584

GRK4 (tGFP-tagged) - Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 4

Product Images

Circular map for RG238584

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238584 representing NM_005307.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGAAAAGGATTATAGCAGT
  CTTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTCTGTGATACCAAACCCACTCTAAAG
  AGGCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCGATGATGAGGACCGAAGTGATTGT
  GGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCCTTTACCAGAAATACCTCCAGAT
  GTTGTGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAAAAAGCCTTTGAGGAATGTACT
  AGAGTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAGAAAGCTCATATTTTTCTCAG
  TTTTTACAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATTTAGACATTACAGAGTTCTA
  GGAAAAGGCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGAAAAATGTATGCCTGCAAA
  AAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAAATGAGAAAAGAATTCTG
  GAGAAAGTGCAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAAAGATGCCTTGTGCTTG
  GTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGCAATCCCGGCTTTGAT
  GAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTACAGAGGGAAAGAATT
  GTATACAGAGACTTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCGGATTTCAGACCTC
  GGTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTCGGCTACATGGCA
  CCTGAAGTTGTCAATAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCTGTCTGATCTAT
  GAAATGATTCAGGGACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGAGGTCGATCAA
  AGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATCTGCAGGATG
  TTACTCACCAAGAATCCAAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGAAGCAGCAC
  CCCGTGTTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTGTCCTGAT
  CCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCGAGCAGTTCTCGGTGGTGAAAGGGATCTACCTG
  GACACCGCAGATGAAGACTTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGAATGAG
  GGCTGCCTGACCATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238584
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MELENIVANSLLLKARQEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDC
  GLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQ
  FLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRIL
  EKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERI
  VYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIY
  EMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQH
  PVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTADEDFYARFATGCVSIPWQNE
  GCLTMVPSEKEVEPKQC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005307
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_005307.3
• RefSeq Size: 2087 bp
• RefSeq ORF: 1503 bp
• Locus ID: 2868
• UniProt ID: P32298
• Cytogenetics: 4p16.3
• Domains: RGS, pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 58.1 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]