ACSF3 (NM_001284316) Human Tagged ORF Clone

CAT#: RG237368

ACSF3 (tGFP-tagged) - Human acyl-CoA synthetase family member 3 (ACSF3), transcript variant 3

Product Images

Circular map for RG237368

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ACSF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG237368 representing NM_001284316.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGCCTGAGTTCAGCCCTCAGCAGGTTTGGGAAAAGTTCTTAAGTTCTGAAACGCCGCGGATCAAT
  GTCTTTATGGCAGTGCCTACAATATACACCAAGCTGATGGAGTACTACGACAGGCATTTTACCCAGCCG
  CACGCCCAGGATTTCTTGCGTGCAGTTTGTGAAGAAAAAATTAGGCTGATGGTCTCAGGCTCAGCTGCC
  CTGCCCCTCCCAGTGCTGGAGAAGTGGAAGAACATCACGGGCCACACCCTGCTGGAGCGGTATGGCATG
  ACCGAGATCGGCATGGCTCTGTCCGGGCCCCTGACCACTGCCGTGCGCCTGCCAGGTTCCGTGGGGACC
  CCACTGCCTGGAGTACAGGTGCGCATTGTCTCAGAAAACCCACAGAGGGAAGCCTGCTCCTACACCATC
  CACGCAGAGGGAGACGAGAGGGGGACCAAGGTGACCCCAGGGTTTGAAGAAAAGGAGGGGGAGCTGCTG
  GTGAGGGGACCCTCCGTGTTTCGAGAATACTGGAATAAACCAGAAGAAACTAAGAGTGCATTCACCCTG
  GATGGCTGGTTTAAGACAGGGGACACCGTGGTGTTTAAGGATGGCCAGTACTGGATCCGAGGCCGGACC
  TCAGTGGACATCATCAAGACTGGAGGCTACAAGGTCAGCGCCCTGGAGGTGGAGTGGCACCTGCTGGCC
  CACCCCAGCATCACAGATGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCCAGCGGGTCACTGCT
  GTGGTGACCCTCCGAGAAGGACACTCACTGTCCCACAGGGAGCTCAAAGAGTGGGCCAGAAATGTCCTG
  GCCCCGTACGCGGTGCCCTCGGAGCTGGTGCTGGTGGAGGAGATCCCGCGGAACCAGATGGGCAAGATT
  GACAAGAAGGCGCTCATCAGGCACTTCCACCCCTCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG237368
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MMPEFSPQQVWEKFLSSETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAA
  LPLPVLEKWKNITGHTLLERYGMTEIGMALSGPLTTAVRLPGSVGTPLPGVQVRIVSENPQREACSYTI
  HAEGDERGTKVTPGFEEKEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTVVFKDGQYWIRGRT
  SVDIIKTGGYKVSALEVEWHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGHSLSHRELKEWARNVL
  APYAVPSELVLVEEIPRNQMGKIDKKALIRHFHPS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001284316
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NM_001284316.2
• RefSeq Size: 2888 bp
• RefSeq ORF: 936 bp
• Locus ID: 197322
• Cytogenetics: 16q24.3
• MW: 35.6 kDa
• Gene Summary: This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]