ACSF3 (NM_001127214) Human Tagged ORF Clone

CAT#: RG225974

ACSF3 (tGFP-tagged) - Human acyl-CoA synthetase family member 3 (ACSF3), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for RG225974

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ACSF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225974 representing NM_001127214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCATGTGGTGCTCACCTTCCGGCGCCTGGGCTGCGCCTTGGCGTCCTGCCGGCTGGCGCCTG
  CGAGACACAGAGGAAGTGGTCTTCTGCACACAGCCCCAGTGGCCCGCTCGGACAGGAGCGCCCCGGTGTT
  CACCCGTGCCCTGGCCTTTGGGGACAGAATCGCCCTGGTTGACCAGCACGGCCGCCACACGTACAGGGAG
  CTTTATTCCCGCAGCCTTCGCCTGTCCCAGGAGATCTGCAGGCTCTGCGGGTGTGTCGGCGGGGACCTCC
  GGGAGGAGAGGGTCTCCTTCCTATGCGCTAACGATGCCTCCTACGTCGTGGCCCAGTGGGCGTCATGGAT
  GAGTGGCGGTGTGGCAGTCCCCCTCTACAGGAAGCATCCCGCGGCCCAGCTGGAGTATGTCATCTGCGAC
  TCCCAGAGCTCTGTGGTCCTTGCCAGCCAGGAGTACCTGGAGCTCCTGAGCCCGGTGGTCAGGAAGCTGG
  GGGTCCCGCTGCTGCCGCTCACACCAGCCATCTACACTGGAGCAGTAGAGGAACCGGCAGAGGTCCCGGT
  CCCAGAGCAGGGATGGAGGAACAAGGGCGCCATGATCATCTACACCAGTGGGACCACGGGGAGGCCCAAG
  GGCGTGCTGAGCACGCACCAAAACATCAGGGCTGTGGTGACCGGGCTGGTCCACAAGTGGGCATGGACCA
  AAGACGACGTGATCCTCCACGTGCTCCCGCTGCACCACGTCCATGGTGTGGTCAACGCGCTGCTCTGTCC
  TCTCTGGGTGGGAGCCACCTGTGTGATGATGCCTGAGTTCAGCCCTCAGCAGGTTTGGGAAAAGTTCTTA
  AGTTCTGAAACGCCGCGGATCAATGTCTTTATGGCAGTGCCTACAATATACACCAAGCTGATGGAGTACT
  ACGACAGGCATTTTACCCAGCCGCACGCCCAGGATTTCTTGCGTGCAGTTTGTGAAGAAAAAATTAGGCT
  GATGGTCTCAGGCTCAGCTGCCCTGCCCCTCCCAGTGCTGGAGAAGTGGAAGAACATCACGGGCCACACC
  CTGCTGGAGCGGTATGGCATGACCGAGATCGGCATGGCTCTGTCCGGGCCCCTGACCACTGCCGTGCGCC
  TGCCAGGTTCCGTGGGGACCCCACTGCCTGGAGTACAGGTGCGCATTGTCTCAGAAAACCCACAGAGGGA
  AGCCTGCTCCTACACCATCCACGCAGAGGGAGACGAGAGGGGGACCAAGGTGACCCCAGGGTTTGAAGAA
  AAGGAGGGGGAGCTGCTGGTGAGGGGACCCTCCGTGTTTCGAGAATACTGGAATAAACCAGAAGAAACTA
  AGAGTGCATTCACCCTGGATGGCTGGTTTAAGACAGGGGACACCGTGGTGTTTAAGGATGGCCAGTACTG
  GATCCGAGGCCGGACCTCAGTGGACATCATCAAGACTGGAGGCTACAAGGTCAGCGCCCTGGAGGTGGAG
  TGGCACCTGCTGGCCCACCCCAGCATCACAGATGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCC
  AGCGGGTCACTGCTGTGGTGACCCTCCGAGAAGGACACTCACTGTCCCACAGGGAGCTCAAAGAGTGGGC
  CAGAAATGTCCTGGCCCCGTACGCGGTGCCCTCGGAGCTGGTGCTGGTGGAGGAGATCCCGCGGAACCAG
  ATGGGCAAGATTGACAAGAAGGCGCTCATCAGGCACTTCCACCCCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225974 representing NM_001127214
  Red=Cloning site Green=Tags(s)
  
  MLPHVVLTFRRLGCALASCRLAPARHRGSGLLHTAPVARSDRSAPVFTRALAFGDRIALVDQHGRHTYRE
  LYSRSLRLSQEICRLCGCVGGDLREERVSFLCANDASYVVAQWASWMSGGVAVPLYRKHPAAQLEYVICD
  SQSSVVLASQEYLELLSPVVRKLGVPLLPLTPAIYTGAVEEPAEVPVPEQGWRNKGAMIIYTSGTTGRPK
  GVLSTHQNIRAVVTGLVHKWAWTKDDVILHVLPLHHVHGVVNALLCPLWVGATCVMMPEFSPQQVWEKFL
  SSETPRINVFMAVPTIYTKLMEYYDRHFTQPHAQDFLRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHT
  LLERYGMTEIGMALSGPLTTAVRLPGSVGTPLPGVQVRIVSENPQREACSYTIHAEGDERGTKVTPGFEE
  KEGELLVRGPSVFREYWNKPEETKSAFTLDGWFKTGDTVVFKDGQYWIRGRTSVDIIKTGGYKVSALEVE
  WHLLAHPSITDVAVIGVPDMTWGQRVTAVVTLREGHSLSHRELKEWARNVLAPYAVPSELVLVEEIPRNQ
  MGKIDKKALIRHFHPS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127214
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127214.4
• RefSeq Size: 3495 bp
• RefSeq ORF: 1731 bp
• Locus ID: 197322
• UniProt ID: Q4G176
• Cytogenetics: 16q24.3
• Gene Summary: This gene encodes a member of the acyl-CoA synthetase family of enzymes that activate fatty acids by catalyzing the formation of a thioester linkage between fatty acids and coenzyme A. The encoded protein is localized to mitochondria, has high specificity for malonate and methylmalonate and possesses malonyl-CoA synthetase activity. Mutations in this gene are a cause of combined malonic and methylmalonic aciduria. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Sep 2013]