PER2 (NM_022817) Human Tagged ORF Clone

CAT#: RG223694

PER2 (tGFP-tagged) - Human period homolog 2 (Drosophila) (PER2)

Product Images

Circular map for RG223694

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PER2
• Synonyms: FASPS; FASPS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223694 representing NM_022817
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGATACGCGGAATTTCCGCCCAGCCCCAGTAACCCCACCAAGGAGCCCGTGGAGCCCCAGCCCA
  GCCAGGTCCCACTGCAGGAAGATGTGGACATGAGCAGTGGCTCCAGTGGACATGAGACCAACGAAAACTG
  CTCCACGGGGCGGGACTCGCAGGGCAGTGACTGTGACGACAGTGGGAAGGAGCTGGGGATGCTGGTGGAG
  CCACCGGATGCCCGCCAGAGTCCAGATACCTTTAGCCTGATGATGGCAAAATCTGAACACAACCCATCTA
  CAAGTGGCTGCAGTAGCGACCAGTCTTCGAAAGTGGACACACACAAAGAACTGATAAAAACACTAAAGGA
  GCTGAAGGTCCACCTCCCTGCAGACAAGAAGGCCAAGGGCAAGGCCAGTACGCTGGCCACCTTGAAGTAC
  GCCCTCAGGAGCGTGAAGCAGGTGAAAGCCAATGAAGAGTATTACCAGCTGCTGATGTCCAGCGAGGGTC
  ACCCCTGTGGAGCAGACGTGCCCTCCTACACCGTGGAGGAGATGGAGAGCGTTACCTCTGAGCACATTGT
  GAAGAATGCCGATATGTTTGCGGTGGCCGTGTCCCTGGTGTCTGGGAAGATCCTGTACATCTCTGACCAG
  GTTGCATCCATATTTCACTGTAAAAGAGATGCCTTCAGCGATGCCAAGTTTGTGGAGTTCCTGGCGCCTC
  ACGATGTGGGCGTGTTCCACAGTTTCACCTCCCCGTACAAGCTTCCCTTGTGGAGCATGTGCAGTGGAGC
  AGATTCTTTTACTCAAGAATGCATGGAGGAGAAATCTTTCTTTTGCCGTGTCAGTGTCCGGAAAAGCCAC
  GAGAATGAAATCCGCTACCACCCCTTCCGCATGACGCCCTACCTGGTCAAGGTGCGGGACCAACAAGGTG
  CTGAGAGTCAGCTTTGCTGCCTTCTGCTGGCAGAGAGAGTGCACTCTGGTTATGAAGCCCCTAGAATTCC
  TCCTGAAAAGAGAATTTTTACAACCACCCATACACCAAATTGTTTGTTCCAGGATGTGGATGAAAGGGCG
  GTCCCTCTCCTGGGCTACCTACCTCAGGACCTGATTGAAACCCCAGTGCTCGTGCAGCTCCACCCTAGTG
  ACAGGCCCTTGATGCTGGCCATCCACAAAAAGATCCTGCAGTCAGGCGGGCAGCCTTTCGACTATTCTCC
  CATTCGGTTTCGCGCCCGGAACGGAGAGTACATCACGTTGGACACCAGCTGGTCCAGCTTCATCAACCCA
  TGGAGCAGGAAAATCTCCTTCATCATTGGGAGGCACAAAGTCAGGGTGGGCCCTTTGAATGAGGACGTGT
  TTGCAGCCCACCCCTGCACAGAGGAGAAGGCCCTGCACCCCAGCATTCAGGAGCTCACAGAGCAGATCCA
  CCGGCTCCTGCTGCAGCCCGTCCCCCACAGCGGCTCCAGTGGCTACGGGAGTCTGGGCAGCAACGGGTCC
  CACGAGCACCTTATGAGCCAGACCTCCTCCAGCGACAGCAACGGCCATGAGGACTCACGCCGGAGGAGAG
  CCGAAATTTGTAAAAATGGTAACAAGACCAAAAATAGAAGTCATTATTCTCATGAATCTGGAGAACAAAA
  GAAAAAATCCGTTACAGAAATGCAAACTAATCCCCCAGCTGAGAAGAAAGCTGTCCCTGCCATGGAAAAG
  GACAGCCTGGGGGTCAGCTTCCCCGAGGAGTTGGCCTGCAAGAACCAGCCCACCTGCTCCTACCAGCAGA
  TCAGCTGCTTGGACAGCGTCATCAGGTACTTGGAGAGCTGCAATGAGGCTGCCACCCTGAAGAGGAAATG
  CGAGTTCCCAGCAAACGTCCCAGCGCTAAGGTCCAGTGATAAGCGGAAGGCCACAGTCAGCCCAGGGCCA
  CACGCTGGAGAGGCAGAGCCGCCCTCCAGGGTGAACAGCCGCACGGGAGTAGGTACGCACCTGACCTCGC
  TGGCACTGCCGGGCAAGGCAGAGAGTGTGGCGTCGCTCACCAGCCAGTGCAGCTACAGCAGCACCATCGT
  CCATGTGGGAGACAAGAAGCCGCAGCCGGAGTTAGAGATGGTGGAAGATGCTGCGAGTGGGCCAGAATCC
  CTGGACTGCCTGGCGGGCCCTGCCCTGGCCTGTGGTCTCAGCCAAGAGAAGGAGCCCTTCAAGAAGCTGG
  GCCTCACCAAGGAGGTACTCGCTGCACACACACAGAAGGAGGAGCAGAGCTTCCTGCAGAAGTTCAAAGA
  AATAAGAAAACTCAGCATTTTCCAGTCCCACTGCCATTACTACTTGCAAGAAAGATCCAAGGGGCAGCCA
  AGTGAACGAACTGCCCCTGGACTAAGAAATACTTCCGGAATAGATTCACCTTGGAAAAAAACAGGAAAGA
  ACAGAAAATTGAAGTCCAAGCGGGTCAAACCTCGAGACTCATCTGAGAGCACCGGATCTGGGGGGCCCGT
  GTCCGCCCGGCCCCCGCTGGTGGGCTTGAACGCCACAGCCTGGTCACCCTCAGACACGTCCCAGTCCAGC
  TGCCCAGCCGTGCCCTTTCCCGCCCCAGTGCCAGCAGCTTATTCACTGCCCGTGTTTCCAGCGCCAGGGA
  CTGTGGCAGCACCCCCGGCACCTCCCCACGCCAGCTTCACAGTGCCTGCTGTGCCCGTGGACCTCCAGCA
  CCAGTTTGCAGTCCAGCCCCCACCTTTCCCTGCCCCTTTGGCGCCTGTCATGGCATTCATGCTACCCAGT
  TATTCCTTCCCCTCGGGGACCCCAAACCTGCCCCAGGCCTTCTTCCCCAGCCAGCCTCAGTTTCCGAGCC
  ACCCCACACTCACATCCGAGATGGCCTCTGCCTCACAGCCTGAGTTCCCCAGCCGGACCTCGATCCCCAG
  ACAGCCATGTGCTTGTCCAGCCACCCGGGCCACCCCACCATCGGCCATGGGTAGGGCCTCCCCACCGCTC
  TTTCAGTCCCGCAGCAGCTCGCCCCTGCAGCTCAACCTGCTGCAGCTGGAGGAAGCCCCTGAGGGTGGCA
  CTGGAGCCATGGGGACCACAGGGGCCACAGAGACAGCAGCTGTAGGGGCGGACTGCAAACCTGGCACTTC
  TCGGGACCAGCAGCCGAAGGCGCCTCTGACCCGTGATGAACCCTCAGACACACAGAACAGTGACGCCCTT
  TCCACGTCAAGCGGCCTCCTAAACCTCCTGCTGAATGAGGACCTCTGCTCAGCCTCGGGCTCTGCTGCTT
  CGGAGTCTCTGGGCTCCGGCTCACTGGGCTGCGACGCCTCCCCGAGTGGGGCAGGCAGTAGTGACACAAG
  TCATACCAGCAAATATTTTGGAAGCATTGACTCCTCAGAGAATAATCACAAAGCAAAAATGAACACTGGT
  ATGGAAGAAAGTGAGCATTTCATTAAGTGCGTCCTGCAGGATCCCATCTGGCTGCTGATGGCAGATGCGG
  ACAGCAGCGTCATGATGACGTACCAGCTGCCTTCCCGAAATTTAGAAGCGGTTTTGAAGGAGGACAGAGA
  GAAGCTGAAGCTCCTACAGAAACTCCAGCCCAGGTTCACGGAGAGTCAGAAGCAGGAGCTGCGCGAGGTC
  CACCAGTGGATGCAGACGGGCGGCCTGCCCGCAGCCATCGACGTGGCAGAATGTGTTTACTGTGAAAACA
  AGGAAAAAGGTAATATTTGCATACCATATGAGGAAGATATTCCTTCTCTGGGACTCAGCGAAGTGTCGGA
  CACCAAAGAAGACGAAAATGGATCCCCCTTGAATCACAGGATCGAAGAGCAGACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223694 representing NM_022817
  Red=Cloning site Green=Tags(s)
  
  MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTGRDSQGSDCDDSGKELGMLVE
  PPDARQSPDTFSLMMAKSEHNPSTSGCSSDQSSKVDTHKELIKTLKELKVHLPADKKAKGKASTLATLKY
  ALRSVKQVKANEEYYQLLMSSEGHPCGADVPSYTVEEMESVTSEHIVKNADMFAVAVSLVSGKILYISDQ
  VASIFHCKRDAFSDAKFVEFLAPHDVGVFHSFTSPYKLPLWSMCSGADSFTQECMEEKSFFCRVSVRKSH
  ENEIRYHPFRMTPYLVKVRDQQGAESQLCCLLLAERVHSGYEAPRIPPEKRIFTTTHTPNCLFQDVDERA
  VPLLGYLPQDLIETPVLVQLHPSDRPLMLAIHKKILQSGGQPFDYSPIRFRARNGEYITLDTSWSSFINP
  WSRKISFIIGRHKVRVGPLNEDVFAAHPCTEEKALHPSIQELTEQIHRLLLQPVPHSGSSGYGSLGSNGS
  HEHLMSQTSSSDSNGHEDSRRRRAEICKNGNKTKNRSHYSHESGEQKKKSVTEMQTNPPAEKKAVPAMEK
  DSLGVSFPEELACKNQPTCSYQQISCLDSVIRYLESCNEAATLKRKCEFPANVPALRSSDKRKATVSPGP
  HAGEAEPPSRVNSRTGVGTHLTSLALPGKAESVASLTSQCSYSSTIVHVGDKKPQPELEMVEDAASGPES
  LDCLAGPALACGLSQEKEPFKKLGLTKEVLAAHTQKEEQSFLQKFKEIRKLSIFQSHCHYYLQERSKGQP
  SERTAPGLRNTSGIDSPWKKTGKNRKLKSKRVKPRDSSESTGSGGPVSARPPLVGLNATAWSPSDTSQSS
  CPAVPFPAPVPAAYSLPVFPAPGTVAAPPAPPHASFTVPAVPVDLQHQFAVQPPPFPAPLAPVMAFMLPS
  YSFPSGTPNLPQAFFPSQPQFPSHPTLTSEMASASQPEFPSRTSIPRQPCACPATRATPPSAMGRASPPL
  FQSRSSSPLQLNLLQLEEAPEGGTGAMGTTGATETAAVGADCKPGTSRDQQPKAPLTRDEPSDTQNSDAL
  STSSGLLNLLLNEDLCSASGSAASESLGSGSLGCDASPSGAGSSDTSHTSKYFGSIDSSENNHKAKMNTG
  MEESEHFIKCVLQDPIWLLMADADSSVMMTYQLPSRNLEAVLKEDREKLKLLQKLQPRFTESQKQELREV
  HQWMQTGGLPAAIDVAECVYCENKEKGNICIPYEEDIPSLGLSEVSDTKEDENGSPLNHRIEEQT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022817
• ORF Size: 3765 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022817.3
• RefSeq Size: 6219 bp
• RefSeq ORF: 3768 bp
• Locus ID: 8864
• UniProt ID: O15055
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• Gene Summary: This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene may increase the risk of getting certain cancers and have been linked to sleep disorders. [provided by RefSeq, Jan 2014]