ABLIM1 (NM_001003407) Human Tagged ORF Clone

CAT#: RG222450

ABLIM1 (tGFP-tagged) - Human actin binding LIM protein 1 (ABLIM1), transcript variant 2

Product Images

Circular map for RG222450

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ABLIM1
• Synonyms: ABLIM; abLIM-1; LIMAB1; LIMATIN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222450 representing NM_001003407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAATGACCTTGGAAATGACTGAGCTCACGGACCCTCATCACACCATGGGAGACTACAAAGTGGCCC
  ACCCTCAGGACCCTCACCACCCATCAGAGAAGCCTGTCATTCACTGCCATAAATGTGGGGAGCCTTGCAA
  GGGTGAAGTGCTTCGGGTCCAGACCAAACATTTCCACATCAAGTGTTTCACCTGCAAAGTGTGTGGCTGT
  GACCTGGCACAAGGGGGCTTCTTCATAAAGAACGGAGAGTATCTCTGCACCCTGGACTACCAGCGGATGT
  ACGGGACACGCTGCCATGGCTGTGGGGAGTTCGTGGAGGGCGAAGTGGTGACTGCTCTGGGCAAGACCTA
  CCATCCCAATTGCTTTGCTTGTACTATCTGCAAGCGCCCGTTTCCACCCGGAGACCGAGTCACATTCAAT
  GGGAGAGACTGCCTTTGTCAACTCTGTGCACAGCCGATGTCGTCCAGTCCGAAAGAAACCACCTTCTCCA
  GCAATTGTGCCGGCTGCGGAAGAGATATCAAGAATGGGCAGGCGCTGCTGGCGCTGGATAAGCAGTGGCA
  CTTGGGGTGCTTTAAATGCAAGTCCTGCGGGAAGGTCCTCACCGGGGAGTACATCAGCAAGGATGGTGCT
  CCGTACTGTGAAAAGGACTACCAGGGACTCTTTGGGGTGAAATGTGAGGCGTGTCACCAGTTTATCACAG
  GGAAAGTCCTGGAGGCAGGTGACAAACATTACCACCCCAGCTGTGCACGATGCAGCAGATGCAACCAGAT
  GTTCACAGAAGGAGAGGAAATGTATCTTCAAGGCTCCACCGTTTGGCATCCCGACTGTAAGCAATCTACG
  AAGACCGAGGAAAAGCTGCGGCCTACCAGGACATCCTCGGAAAGTATTTATTCTAGGCCAGGCTCCAGTA
  TTCCTGGCTCACCAGGTCATACTATCTATGCAAAAGTAGACAATGAGATCCTGGATTACAAGGATTTAGC
  AGCCATTCCGAAGGTCAAGGCAATTTATGACATTGAACGTCCAGATCTTATTACCTATGAGCCTTTCTAC
  ACTTCGGGCTATGATGACAAACAGGAGAGACAGAGCCTTGGAGAGTCTCCGAGGACTTTGTCTCCTACTC
  CATCAGCAGAAGGGTACCAGGATGTTCGGGATCGGATGATCCATCGGTCCACGAGCCAGGGCTCCATCAA
  CTCCCCTGTGTACAGCCGCCACAGCTACACTCCAACCACGTCCCGCTCTCCCCAGCATTTCCACAGACCT
  GGCAATGAGCCGTCCAGCGGCCGGAACTCCCCTCTCCCTTACCGGCCAGACAGCCGCCCTCTAACTCCAA
  CTTACGCTCAGGCCCCTAAACATTTCCATGTTCCAGATCAAGGAATCAACATTTACCGAAAGCCACCCAT
  CTACAAACAGCATGCTGCCTTGGCAGCCCAGAGCAAGTCCTCAGAAGATATCATCAAGTTTTCCAAGTTC
  CCAGCAGCCCAGGCACCAGACCCCAGCGAGACACCAAAGATTGAGACGGACCACTGGCCTGGTCCCCCCT
  CATTTGCTGTCGTAGGACCTGACATGAAACGCAGATCTAGTGGCAGAGAGGAAGATGATGAGGAACTTCT
  GAGACGTCGGCAGCTTCAAGAAGAGCAATTAATGAAGCTTAACTCAGGCCTGGGACAGTTGATCTTGAAA
  GAAGAGATGGAGAAAGAGAGCCGGGAAAGGTCATCTCTGTTAGCCAGTCGCTACGATTCTCCCATCAACT
  CAGCTTCACATATTCCATCATCTAAAACTGCATCTCTCCCTGGCTATGGAAGAAATGGGCTTCACCGGCC
  TGTTTCTACCGACTTCGCTCAGTATAACAGCTATGGGGATGTCAGCGGGGGAGTGCGAGATTACCAGACA
  CTCCCAGATGGCCACATGCCTGCAATGAGAATGGACCGAGGAGTGTCTATGCCCAACATGTTGGAACCAA
  AGATATTTCCATATGAAATGCTCATGGTGACCAACAGAGGGCGAAACAAAATCCTCAGAGAGGTGGACAG
  AACCAGGCTGGAGCGCCACTTAGCCCCTGAAGTGTTTCGGGAAATCTTTGGAATGTCCATACAGGAGTTT
  GACAGGTTACCTCTTTGGAGACGCAACGACATGAAGAAAAAAGCAAAACTCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222450 representing NM_001003407
  Red=Cloning site Green=Tags(s)
  
  MLMTLEMTELTDPHHTMGDYKVAHPQDPHHPSEKPVIHCHKCGEPCKGEVLRVQTKHFHIKCFTCKVCGC
  DLAQGGFFIKNGEYLCTLDYQRMYGTRCHGCGEFVEGEVVTALGKTYHPNCFACTICKRPFPPGDRVTFN
  GRDCLCQLCAQPMSSSPKETTFSSNCAGCGRDIKNGQALLALDKQWHLGCFKCKSCGKVLTGEYISKDGA
  PYCEKDYQGLFGVKCEACHQFITGKVLEAGDKHYHPSCARCSRCNQMFTEGEEMYLQGSTVWHPDCKQST
  KTEEKLRPTRTSSESIYSRPGSSIPGSPGHTIYAKVDNEILDYKDLAAIPKVKAIYDIERPDLITYEPFY
  TSGYDDKQERQSLGESPRTLSPTPSAEGYQDVRDRMIHRSTSQGSINSPVYSRHSYTPTTSRSPQHFHRP
  GNEPSSGRNSPLPYRPDSRPLTPTYAQAPKHFHVPDQGINIYRKPPIYKQHAALAAQSKSSEDIIKFSKF
  PAAQAPDPSETPKIETDHWPGPPSFAVVGPDMKRRSSGREEDDEELLRRRQLQEEQLMKLNSGLGQLILK
  EEMEKESRERSSLLASRYDSPINSASHIPSSKTASLPGYGRNGLHRPVSTDFAQYNSYGDVSGGVRDYQT
  LPDGHMPAMRMDRGVSMPNMLEPKIFPYEMLMVTNRGRNKILREVDRTRLERHLAPEVFREIFGMSIQEF
  DRLPLWRRNDMKKKAKLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003407
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003407.2
• RefSeq Size: 7620 bp
• RefSeq ORF: 2157 bp
• Locus ID: 3983
• UniProt ID: O14639
• Cytogenetics: 10q25.3
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a LIM zinc-binding domain-containing protein that binds to actin filaments and mediates interactions between actin and cytoplasmic targets. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2017]