CED6 (GULP1) (NM_016315) Human Tagged ORF Clone

CAT#: RG221580

GULP1 (tGFP-tagged) - Human GULP, engulfment adaptor PTB domain containing 1 (GULP1)

Product Images

Circular map for RG221580

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CED6
• Synonyms: CED-6; CED6; GULP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221580 representing NM_016315
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGTGCTTTTAGCAGGAAGAAAGACAAAACATGGATGCATACACCTGAAGCTTTATCAAAACATT
  TCATTCCCTATAATGCAAAGTTTCTTGGCAGTACAGAAGTGGAACAGCCAAAAGGAACAGAAGTTGTGAG
  AGATGCTGTAAGGAAACTAAAGTTTGCAAGACATATCAAGAAATCTGAAGGCCAGAAAATTCCTAAAGTG
  GAGTTGCAAATATCAATTTATGGAGTAAAAATTCTAGAACCCAAAACAAAGGAAGTTCAACACAATTGCC
  AGCTTCATAGAATATCTTTTTGTGCAGATGATAAAACTGACAAGAGGATATTCACTTTCATATGCAAAGA
  TTCTGAGTCAAATAAACATTTGTGCTATGTATTTGACAGCGAAAAGTGTGCTGAAGAGATCACTTTAACA
  ATTGGCCAAGCATTTGACCTGGCATACAGGAAATTTCTAGAATCAGGAGGAAAAGATGTTGAAACAAGAA
  AACAGATCGCAGGGTTACAAAAAAGAATCCAAGACTTAGAAACAGAAAATATGGAACTTAAAAATAAAGT
  ACAAGATTTGGAAAACCAACTGAGAATAACTCAAGTATCAGCACCTCCAGCAGGCAGTATGACACCTAAG
  TCGCCCTCCACTGACATCTTTGATATGATTCCATTTTCTCCAATATCACACCAGTCTTCGATGCCTACTC
  GCAATGGCACACAGCCACCTCCAGTACCTAGTAGATCTACTGAGATTAAACGGGACCTGTTTGGAGCAGA
  ACCTTTTGACCCATTTAACTGTGGAGCAGCAGATTTCCCTCCAGATATTCAATCAAAATTAGATGAGATG
  CAGGAGGGGTTCAAAATGGGACTAACTCTTGAAGGCACAGTATTTTGTCTCGACCCGTTAGACAGTAGGT
  GC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221580 representing NM_016315
  Red=Cloning site Green=Tags(s)
  
  MNRAFSRKKDKTWMHTPEALSKHFIPYNAKFLGSTEVEQPKGTEVVRDAVRKLKFARHIKKSEGQKIPKV
  ELQISIYGVKILEPKTKEVQHNCQLHRISFCADDKTDKRIFTFICKDSESNKHLCYVFDSEKCAEEITLT
  IGQAFDLAYRKFLESGGKDVETRKQIAGLQKRIQDLETENMELKNKVQDLENQLRITQVSAPPAGSMTPK
  SPSTDIFDMIPFSPISHQSSMPTRNGTQPPPVPSRSTEIKRDLFGAEPFDPFNCGAADFPPDIQSKLDEM
  QEGFKMGLTLEGTVFCLDPLDSRC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016315
• ORF Size: 912 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016315.4
• RefSeq Size: 3464 bp
• RefSeq ORF: 915 bp
• Locus ID: 51454
• UniProt ID: Q9UBP9
• Cytogenetics: 2q32.1-q32.2
• Domains: PID
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is an adapter protein necessary for the engulfment of apoptotic cells by phagocytes. Several transcript variants, some protein coding and some thought not to be protein coding, have been found for this gene. [provided by RefSeq, Nov 2011]