Tapasin (TAPBP) (NM_172208) Human Tagged ORF Clone
CAT#: RG219599
- TrueORF®
TAPBP (tGFP-tagged) - Human TAP binding protein (tapasin) (TAPBP), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_172208" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Tapasin |
Synonyms | NGS17; TAPA; TPN; TPSN |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG219599 representing NM_172208
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCCCTGTCTCTGCTCCTCGCTGTGGCTTTGGGCCTGGCGACCGCCGTCTCAGCAGGACCCGCGG TGATCGAGTGTTGGTTCGTGGAGGATGCGAGCGGAAAGGGCCTGGCCAAGAGACCCGGTGCACTGCTGTT GCGCCAGGGACCGGGGGAACCGCCGCCCCGGCCGGACCTCGACCCTGAGCTCTATCTCAGTGTACACGAC CCCGCGGGCGCCCTCCAGGCTGCCTTCAGGCGGTATCCCCGGGGCGCCCCCGCACCACACTGCGAGATGA GCCGCTTCGTGCCTCTCCCCGCCTCTGCGAAATGGGCCAGCGGCCTGACCCCCGCGCAGAACTGCCCGCG GGCCCTGGATGGGGCTTGGCTGATGGTCAGCATATCCAGCCCAGTCCTCAGCCTCTCCAGCCTCTTGCGA CCACAGCCAGAGCCTCAGCAGGAGCCTGTTCTCATCACCATGGCAACAGTGGTACTGACTGTCCTCACCC ACACCCCTGCCCCTCGAGTGAGACTGGGACAAGATGCTCTGCTGGACTTGAGCTTTGCCTACATGCCCCC CACCTCCGAGGCCGCCTCATCTCTGGCTCCGGGTCCCCCTCCCTTTGGGCTAGAGTGGCGACGCCAGCAC CTGGGTAAGGGACATCTGCTCCTGGCTGCAACTCCTGGGCTGAATGGCCAGATGCCAGCAGCCCAAGAAG GGGCCGTGGCATTTGCTGCTTGGGATGATGATGAGCCATGGGGCCCATGGACCGGAAATGGGACCTTCTG GCTGCCTACAGTTCAACCCTTTCAGGAGGGCACCTATCTGGCCACCATACACCTGCCATACCTGCAAGGA CAGGTCACCCTGGAGCTTGCTGTGTACAGTGAGTTGGGGGACAGAGGTCTCCAGGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG219599 representing NM_172208
Red=Cloning site Green=Tags(s) MKSLSLLLAVALGLATAVSAGPAVIECWFVEDASGKGLAKRPGALLLRQGPGEPPPRPDLDPELYLSVHD PAGALQAAFRRYPRGAPAPHCEMSRFVPLPASAKWASGLTPAQNCPRALDGAWLMVSISSPVLSLSSLLR PQPEPQQEPVLITMATVVLTVLTHTPAPRVRLGQDALLDLSFAYMPPTSEAASSLAPGPPPFGLEWRRQH LGKGHLLLAATPGLNGQMPAAQEGAVAFAAWDDDEPWGPWTGNGTFWLPTVQPFQEGTYLATIHLPYLQG QVTLELAVYSELGDRGLQG TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_172208 |
ORF Size | 897 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172208.1, NP_757345.1 |
RefSeq Size | 2404 bp |
RefSeq ORF | 1515 bp |
Locus ID | 6892 |
UniProt ID | O15533 |
Cytogenetics | 6p21.32 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Antigen processing and presentation |
Gene Summary | This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219599 | TAPBP (Myc-DDK-tagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 2 |
USD 457.00 |
|
RC219599L3 | Lenti-ORF clone of TAPBP (Myc-DDK-tagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 2 |
USD 757.00 |
|
RC219599L4 | Lenti-ORF clone of TAPBP (mGFP-tagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 2 |
USD 757.00 |
|
SC312855 | TAPBP (untagged)-Human TAP binding protein (tapasin) (TAPBP), transcript variant 2 |
USD 517.00 |
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