Xanthine Oxidase (XDH) (NM_000379) Human Tagged ORF Clone

CAT#: RG219402

XDH (tGFP-tagged) - Human xanthine dehydrogenase (XDH)

Product Images

Circular map for RG219402

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xanthine Oxidase
• Synonyms: XAN1; XO; XOR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219402 representing NM_000379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAGACAAATTGGTTTTCTTTGTGAATGGCAGAAAGGTGGTGGAGAAAAATGCAGATCCAGAGA
  CAACCCTTTTGGCCTACCTGAGAAGAAAGTTGGGGCTGAGTGGAACCAAGCTCGGCTGTGGAGAGGGGGG
  CTGCGGGGCTTGCACAGTGATGCTCTCCAAGTATGATCGTCTGCAGAACAAGATCGTCCACTTTTCTGCC
  AATGCCTGCCTGGCCCCCATCTGCTCCTTGCACCATGTTGCAGTGACAACTGTGGAAGGAATAGGAAGCA
  CCAAGACGAGGCTGCATCCTGTGCAGGAGAGAATTGCCAAAAGCCACGGCTCCCAGTGCGGGTTCTGCAC
  CCCTGGCATCGTCATGAGTATGTACACACTGCTCCGGAATCAGCCCGAGCCCACCATGGAGGAGATTGAG
  AATGCCTTCCAAGGAAATCTGTGCCGCTGCACAGGCTACAGACCCATCCTCCAGGGCTTCCGGACCTTTG
  CCAGGGATGGTGGATGCTGTGGAGGAGATGGGAATAATCCAAATTGCTGCATGAACCAGAAGAAAGACCA
  CTCAGTCAGCCTCTCGCCATCTTTATTCAAACCAGAGGAGTTCACGCCCCTGGATCCAACCCAGGAGCCC
  ATTTTTCCCCCAGAGTTGCTGAGGCTGAAAGACACTCCTCGGAAGCAGCTGCGATTTGAAGGGGAGCGTG
  TGACGTGGATACAGGCCTCAACCCTCAAGGAGCTGCTGGACCTCAAGGCTCAGCACCCTGACGCCAAGCT
  GGTCGTGGGGAACACGGAGATTGGCATTGAGATGAAGTTCAAGAATATGCTGTTTCCTATGATTGTCTGC
  CCAGCCTGGATCCCTGAGCTGAATTCGGTAGAACATGGACCCGACGGTATCTCCTTTGGAGCTGCTTGCC
  CCCTGAGCATTGTGGAAAAAACCCTGGTGGATGCTGTTGCTAAGCTTCCTGCCCAAAAGACAGAGGTGTT
  CAGAGGGGTCCTGGAGCAGCTGCGCTGGTTTGCTGGGAAGCAAGTCAAGTCTGTGGCGTCCGTTGGAGGG
  AACATCATCACTGCCAGCCCCATCTCCGACCTCAACCCCGTGTTCATGGCCAGTGGGGCCAAGCTGACAC
  TTGTGTCCAGAGGCACCAGGAGAACTGTCCAGATGGACCACACCTTCTTCCCTGGCTACAGAAAGACCCT
  GCTGAGCCCGGAGGAGATACTGCTCTCCATAGAGATCCCCTACAGCAGGGAGGGGGAGTATTTCTCAGCA
  TTCAAGCAGGCCTCCCGGAGAGAAGATGACATTGCCAAGGTAACCAGTGGCATGAGAGTTTTATTCAAGC
  CAGGAACCACAGAGGTACAGGAGCTGGCCCTTTGCTATGGTGGAATGGCCAACAGAACCATCTCAGCCCT
  CAAGACCACTCAGAGGCAGCTTTCCAAGCTCTGGAAGGAGGAGCTGCTGCAGGACGTGTGTGCAGGACTG
  GCAGAGGAGCTGCATCTGCCTCCCGATGCCCCTGGTGGCATGGTGGACTTCCGGTGCACCCTCACCCTCA
  GCTTCTTCTTCAAGTTCTACCTGACAGTCCTTCAGAAGCTGGGCCAAGAGAACCTGGAAGACAAGTGTGG
  TAAACTGGACCCCACTTTCGCCAGTGCAACTTTACTGTTTCAGAAAGACCCCCCAGCCGATGTCCAGCTC
  TTCCAAGAGGTGCCCAAGGGTCAGTCTGAGGAGGACATGGTGGGCCGGCCCCTGCCCCACCTGGCAGCGG
  ACATGCAGGCCTCTGGTGAGGCCGTGTACTGTGACGACATTCCTCGCTACGAGAATGAGCTGTCTCTCCG
  GCTGGTCACCAGCACCCGGGCCCACGCCAAGATCAAGTCCATAGATACATCAGAAGCTAAGAAGGTTCCA
  GGGTTTGTTTGTTTCATTTCCGCTGATGATGTTCCTGGGAGTAACATAACTGGAATTTGTAATGATGAGA
  CAGTCTTTGCGAAGGATAAGGTTACTTGTGTTGGGCATATCATTGGTGCTGTGGTTGCTGACACCCCGGA
  ACACACACAGAGAGCTGCCCAAGGGGTGAAAATCACCTATGAAGAACTACCAGCCATTATCACAATTGAG
  GATGCTATAAAGAACAACTCCTTTTATGGACCTGAGCTGAAGATCGAGAAAGGGGACCTAAAGAAGGGGT
  TTTCCGAAGCAGATAATGTTGTGTCAGGGGAGATATACATCGGTGGCCAAGAGCACTTCTACCTGGAGAC
  TCACTGCACCATTGCTGTTCCAAAAGGCGAGGCAGGGGAGATGGAGCTCTTTGTGTCTACACAGAACACC
  ATGAAGACCCAGAGCTTTGTTGCAAAAATGTTGGGGGTTCCAGCAAACCGGATTGTGGTTCGAGTGAAGA
  GAATGGGAGGAGGCTTTGGAGGCAAGGAGACCCGGAGCACTGTGGTGTCCACGGCAGTGGCCCTGGCTGC
  ATATAAGACCGGCCGCCCTGTGCGATGCATGCTGGACCGTGATGAGGACATGCTGATAACTGGTGGCAGA
  CATCCCTTCCTGGCCAGATACAAGGTTGGCTTCATGAAGACTGGGACAGTTGTGGCTCTTGAGGTGGACC
  ACTTCAGCAATGTGGGGAACACCCAGGATCTCTCTCAGAGTATTATGGAACGAGCTTTATTCCACATGGA
  CAACTGCTATAAAATCCCCAACATCCGGGGCACTGGGCGGCTGTGCAAAACCAACCTTCCCTCCAACACG
  GCCTTCCGGGGCTTTGGGGGGCCCCAGGGGATGCTCATTGCCGAGTGCTGGATGAGTGAAGTTGCAGTGA
  CCTGTGGGATGCCTGCAGAGGAGGTGCGGAGAAAAAACCTGTACAAAGAAGGGGACCTGACACACTTCAA
  CCAGAAGCTTGAGGGTTTCACCTTGCCCAGATGCTGGGAAGAATGCCTAGCAAGCTCTCAGTATCATGCT
  CGGAAGAGTGAGGTTGACAAGTTCAACAAGGAGAATTGTTGGAAAAAGAGAGGATTGTGCATAATTCCCA
  CCAAGTTTGGAATAAGCTTCACAGTTCCTTTTCTGAATCAGGCAGGAGCCCTACTTCATGTGTACACAGA
  TGGCTCTGTGCTGCTGACCCACGGGGGGACTGAGATGGGCCAAGGCCTTCATACCAAAATGGTCCAGGTG
  GCCAGTAGAGCTCTGAAAATCCCCACCTCTAAGATTTATATCAGCGAGACAAGCACTAACACTGTGCCCA
  ACACCTCTCCCACGGCTGCCTCTGTCAGCGCTGACCTCAATGGACAGGCCGTCTATGCGGCTTGTCAGAC
  CATCTTGAAAAGGCTGGAACCCTACAAGAAGAAGAATCCCAGTGGCTCCTGGGAAGACTGGGTCACAGCT
  GCCTACATGGACACAGTGAGCTTGTCTGCCACTGGGTTTTATAGAACACCCAATCTGGGCTACAGCTTTG
  AGACTAACTCAGGGAACCCCTTCCACTACTTCAGCTATGGGGTGGCTTGCTCTGAAGTAGAAATCGACTG
  CCTAACAGGAGATCATAAGAACCTCCGCACAGATATTGTCATGGATGTTGGCTCCAGTCTAAACCCTGCT
  ATTGATATTGGACAGGTGGAAGGGGCATTTGTCCAGGGCCTTGGCCTCTTCACCCTAGAGGAGCTACACT
  ATTCCCCCGAGGGGAGCCTGCACACCCGTGGCCCTAGCACCTACAAGATCCCGGCATTTGGCAGCATCCC
  CATTGAGTTCAGGGTGTCCCTGCTCCGCGACTGCCCCAACAAGAAGGCCATCTATGCATCGAAGGCTGTT
  GGAGAGCCGCCCCTCTTCCTGGCTGCTTCTATCTTCTTTGCCATCAAAGATGCCATCCGTGCAGCTCGAG
  CTCAGCACACAGGTAATAACGTGAAGGAACTCTTCCGGCTAGACAGCCCTGCCACCCCGGAGAAGATCCG
  CAATGCCTGCGTGGACAAGTTCACCACCCTGTGTGTCACTGGTGTCCCAGAAAACTGCAAACCCTGGTCT
  GTGAGGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219402 representing NM_000379
  Red=Cloning site Green=Tags(s)
  
  MTADKLVFFVNGRKVVEKNADPETTLLAYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRLQNKIVHFSA
  NACLAPICSLHHVAVTTVEGIGSTKTRLHPVQERIAKSHGSQCGFCTPGIVMSMYTLLRNQPEPTMEEIE
  NAFQGNLCRCTGYRPILQGFRTFARDGGCCGGDGNNPNCCMNQKKDHSVSLSPSLFKPEEFTPLDPTQEP
  IFPPELLRLKDTPRKQLRFEGERVTWIQASTLKELLDLKAQHPDAKLVVGNTEIGIEMKFKNMLFPMIVC
  PAWIPELNSVEHGPDGISFGAACPLSIVEKTLVDAVAKLPAQKTEVFRGVLEQLRWFAGKQVKSVASVGG
  NIITASPISDLNPVFMASGAKLTLVSRGTRRTVQMDHTFFPGYRKTLLSPEEILLSIEIPYSREGEYFSA
  FKQASRREDDIAKVTSGMRVLFKPGTTEVQELALCYGGMANRTISALKTTQRQLSKLWKEELLQDVCAGL
  AEELHLPPDAPGGMVDFRCTLTLSFFFKFYLTVLQKLGQENLEDKCGKLDPTFASATLLFQKDPPADVQL
  FQEVPKGQSEEDMVGRPLPHLAADMQASGEAVYCDDIPRYENELSLRLVTSTRAHAKIKSIDTSEAKKVP
  GFVCFISADDVPGSNITGICNDETVFAKDKVTCVGHIIGAVVADTPEHTQRAAQGVKITYEELPAIITIE
  DAIKNNSFYGPELKIEKGDLKKGFSEADNVVSGEIYIGGQEHFYLETHCTIAVPKGEAGEMELFVSTQNT
  MKTQSFVAKMLGVPANRIVVRVKRMGGGFGGKETRSTVVSTAVALAAYKTGRPVRCMLDRDEDMLITGGR
  HPFLARYKVGFMKTGTVVALEVDHFSNVGNTQDLSQSIMERALFHMDNCYKIPNIRGTGRLCKTNLPSNT
  AFRGFGGPQGMLIAECWMSEVAVTCGMPAEEVRRKNLYKEGDLTHFNQKLEGFTLPRCWEECLASSQYHA
  RKSEVDKFNKENCWKKRGLCIIPTKFGISFTVPFLNQAGALLHVYTDGSVLLTHGGTEMGQGLHTKMVQV
  ASRALKIPTSKIYISETSTNTVPNTSPTAASVSADLNGQAVYAACQTILKRLEPYKKKNPSGSWEDWVTA
  AYMDTVSLSATGFYRTPNLGYSFETNSGNPFHYFSYGVACSEVEIDCLTGDHKNLRTDIVMDVGSSLNPA
  IDIGQVEGAFVQGLGLFTLEELHYSPEGSLHTRGPSTYKIPAFGSIPIEFRVSLLRDCPNKKAIYASKAV
  GEPPLFLAASIFFAIKDAIRAARAQHTGNNVKELFRLDSPATPEKIRNACVDKFTTLCVTGVPENCKPWS
  VRV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000379
• ORF Size: 3999 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000379.2, NP_000370.1
• RefSeq Size: 4428 bp
• RefSeq ORF: 4002 bp
• Locus ID: 7498
• UniProt ID: P47989
• Cytogenetics: 2p23.1
• Protein Families: Druggable Genome
• Protein Pathways: Caffeine metabolism, Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Gene Summary: Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]