EPX (NM_000502) Human Tagged ORF Clone

CAT#: RG218959

EPX (tGFP-tagged) - Human eosinophil peroxidase (EPX)

Product Images

Circular map for RG218959

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EPX
• Synonyms: EPO; EPP; EPX-PEN; EPXD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218959 representing NM_000502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTGCTCCCAGCCCTGGCAGGGGTCCTGGCCACACTCGTCCTCGCCCAGCCCTGTGAGGGCACTG
  ACCCAGCCTCCCCTGGGGCAGTGGAGACCTCGGTCCTGCGAGACTGCATAGCAGAGGCCAAGTTGCTGGT
  GGATGCTGCCTACAATTGGACCCAGAAGAGCATCAAGCAGCGGCTTCGCAGCGGTTCAGCCAGCCCCATG
  GACCTCCTGTCCTACTTCAAACAACCGGTAGCAGCCACCAGGACAGTTGTTCGGGCCGCAGATTATATGC
  ATGTGGCTTTGGGGCTGCTTGAAGAGAAGTTACAACCCCAGCGGTCCGGACCCTTCAATGTCACTGATGT
  GCTAACAGAACCACAGCTGCGGCTGCTGTCCCAGGCCAGTGGCTGTGCTCTCCGGGACCAGGCCGAGCGC
  TGCAGCGACAAGTACCGCACCATCACTGGACGGTGCAACAACAAGAGGAGACCCTTGCTAGGGGCCTCCA
  ACCAGGCTCTGGCTCGCTGGCTGCCCGCCGAGTATGAGGATGGGCTGTCGCTCCCCTTCGGCTGGACCCC
  CAGCAGGAGGCGCAATGGCTTCCTTCTCCCTCTTGTCCGGGCTGTCTCCAACCAGATTGTGCGCTTCCCC
  AATGAGAGACTGACCTCCGACCGTGGCCGAGCCCTCATGTTCATGCAGTGGGGCCAGTTCATTGACCATG
  ACCTGGACTTCTCCCCGGAGTCCCCGGCCAGAGTGGCCTTCACTGCAGGCGTTGACTGTGAGAGGACCTG
  CGCCCAGCTGCCCCCCTGCTTTCCCATCAAGATCCCACCCAATGACCCCCGCATCAAGAACCAGCGTGAC
  TGCATCCCTTTCTTCCGCTCGGCACCCTCATGCCCCCAAAACAAGAACAGAGTCCGCAACCAGATCAACG
  CGCTCACCTCCTTTGTGGACGCCAGCATGGTGTATGGCAGTGAGGTCTCCCTCTCGCTGCGGCTCCGCAA
  CCGGACCAACTACCTGGGGCTGCTGGCCATCAACCAGCGCTTTCAAGACAACGGCCGGGCCCTGCTGCCC
  TTCGACAACCTGCACGATGACCCCTGTCTCCTCACCAACCGCTCGGCGCGCATCCCCTGCTTCCTGGCAG
  GTGACACCCGATCAACGGAAACCCCCAAACTGGCAGCCATGCACACCCTCTTTATGCGAGAGCACAACCG
  GCTGGCCACCGAGCTGAGACGCCTGAATCCCCGGTGGAATGGAGACAAACTGTACAATGAGGCTCGGAAG
  ATCATGGGGGCCATGGTCCAGATCATCACCTACCGAGACTTTCTGCCCCTGGTTCTGGGCAAGGCCCGGG
  CCAGGAGAACCCTGGGGCACTACAGGGGGTACTGCTCCAATGTGGACCCACGGGTGGCCAATGTCTTCAC
  CCTGGCCTTCCGCTTTGGCCACACAATGCTCCAGCCCTTCATGTTCCGCTTGGACAGTCAGTACCGGGCC
  TCCGCACCCAACTCGCATGTCCCACTTAGCTCTGCCTTCTTTGCCAGCTGGCGGATCGTGTATGAAGGGG
  GCATCGACCCCATCCTCCGGGGCCTCATGGCCACCCCTGCCAAGCTGAACCGTCAGGATGCCATGTTAGT
  GGATGAGCTCCGGGACCGGCTGTTTCGGCAAGTGAGGAGGATTGGGCTGGACCTGGCAGCTCTCAACATG
  CAACGAAGCCGGGACCACGGCCTTCCAGGGTACAATGCTTGGAGGCGCTTCTGTGGGCTCTCCCAGCCCC
  GGAATTTGGCACAGCTTAGCCGGGTGCTGAAAAACCAGGACTTGGCAAGGAAGTTCCTGAATTTGTATGG
  AACACCTGACAACATTGACATCTGGATTGGGGCCATCGCTGAGCCTCTTTTGCCGGGGGCTCGAGTGGGG
  CCTCTTCTGGCTTGTCTGTTCGAGAACCAGTTCAGAAGAGCCCGAGACGGAGACAGGTTCTGGTGGCAGA
  AACGAGGTGTTTTCACCAAAAGACAGCGCAAGGCCCTGAGCAGAATTTCCTTGTCTCGAATTATATGTGA
  CAATACCGGTATCACCACGGTTTCAAGGGACATCTTCAGAGCCAACATCTACCCTCGGGGCTTTGTGAAC
  TGCAGCCGTATCCCCAGGTTGAACCTATCAGCCTGGCGAGGGACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218959 representing NM_000502
  Red=Cloning site Green=Tags(s)
  
  MHLLPALAGVLATLVLAQPCEGTDPASPGAVETSVLRDCIAEAKLLVDAAYNWTQKSIKQRLRSGSASPM
  DLLSYFKQPVAATRTVVRAADYMHVALGLLEEKLQPQRSGPFNVTDVLTEPQLRLLSQASGCALRDQAER
  CSDKYRTITGRCNNKRRPLLGASNQALARWLPAEYEDGLSLPFGWTPSRRRNGFLLPLVRAVSNQIVRFP
  NERLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFTAGVDCERTCAQLPPCFPIKIPPNDPRIKNQRD
  CIPFFRSAPSCPQNKNRVRNQINALTSFVDASMVYGSEVSLSLRLRNRTNYLGLLAINQRFQDNGRALLP
  FDNLHDDPCLLTNRSARIPCFLAGDTRSTETPKLAAMHTLFMREHNRLATELRRLNPRWNGDKLYNEARK
  IMGAMVQIITYRDFLPLVLGKARARRTLGHYRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRA
  SAPNSHVPLSSAFFASWRIVYEGGIDPILRGLMATPAKLNRQDAMLVDELRDRLFRQVRRIGLDLAALNM
  QRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLLPGARVG
  PLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSRIICDNTGITTVSRDIFRANIYPRGFVN
  CSRIPRLNLSAWRGT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000502
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000502.6
• RefSeq Size: 2731 bp
• RefSeq ORF: 2148 bp
• Locus ID: 8288
• UniProt ID: P11678
• Cytogenetics: 17q22
• Domains: An_peroxidase
• Protein Families: Druggable Genome
• Protein Pathways: Asthma
• Gene Summary: This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Feb 2016]