RALGDS (NM_006266) Human Tagged ORF Clone

CAT#: RG216933

RALGDS (tGFP-tagged) - Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 1

Product Images

Circular map for RG216933

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RALGDS
• Synonyms: RalGEF; RGDS; RGF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216933 representing NM_006266
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGCGCATGTGGGCCGAGGCGGCCGGGCCTGCTGGCGGCGCCGAGCCGCTGTTTCCGGGCTCCC
  GGCGGAGCCGCAGCGTGTGGGACGCCGTGCGCCTGGAGGTGGGCGTCCCCGACAGCTGCCCGGTGGTGCT
  GCACAGCTTCACGCAGCTAGACCCCGACCTGCCGCGCCCGGAGAGCTCCACGCAGGAGATCGGTGAGGAG
  CTGATCAACGGAGTCATCTACTCCATCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGC
  AGCGCTGGCTCGGGTATGAGAATGAGTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAA
  GGCTGGCACGCTGGAGAAGCTGGTGGAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTC
  ACCATCTTCCTGTGTACCTATAGAGCCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGT
  ACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGG
  TGGACCCCAGGACCAACTTAAAAATGCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAG
  GATTTCTGTCAACCTCCGGACTTTCCCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAG
  GCTCAGACCTGGAGCGCCGTGCCCACCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGA
  GCCTGAGGCTCTGTCACCAGTGCCAGCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCT
  CGAGCACCCAGCCCAGTGCCGGCTCCAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAG
  AGCTAGAAGTAGCTCCAGCACCAGCTCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATC
  GGCTCCAGCGCCAGCTCTGGAACTAGAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAG
  CTGGAGCCAGCTCCAGCACCAGTTCCATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACG
  GGCTGAGTGAGGAGAAGCCTCACCTCTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGAT
  GGATGCGGAACTGTTCAAGAAGGTGGTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAG
  AAGGGCAAGGAGCACCTGGCGCCCACCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTG
  TCATCACCACCTGCCTCGGGAACCGAAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGAT
  CGAGGTGGCCAGGGAGTGCCGGATCCTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAG
  AGCAACTCCATCCACCGTCTGAAGAAGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGA
  AGCTGTCAGAGATCTTCTCAGATGAGAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCAC
  CTCCAAGTTTGCCACCCTGGAGATGAACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATC
  CAGGGCACCGTTCCCTACCTGGGCACGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACT
  ATCTGTATGGCAGACTCATCAACTTTGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCT
  GCTGCAGTCGGCCTGCAACAACTACAGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTG
  GAGCGGCTCAGCGAGACTGAGAGCTACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCA
  ACACCCTCAGGACCAAGAAGAACACAGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGA
  GCTCAGTACCAGTGGCAGCTCCCACTCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGC
  GGGGACATCGCTGACGCGCTCAGCGTGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACA
  TCAGCTTCGTCCCGGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCC
  GGAGACCTCCGGCATCAGCTCAGCCTCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCT
  GCCACACGCACCCACAAGCGCTCTGTCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACC
  AGCAGGTGGGCGACTGCTGTATCATCCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCAT
  CCTGGTGACCAGCCAAGATAAGGCTCCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAG
  GAGGAGCCGGAGGACTATGAGCTGCTGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACG
  CCAACGTCTTCTATGCCATGAACTCTACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAA
  GGGAGTGAAGGTCAAGCACGGAGCCAGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCC
  AAGGGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216933 representing NM_006266
  Red=Cloning site Green=Tags(s)
  
  MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEE
  LINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
  TIFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSE
  DFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELALTPA
  RAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALELEPAPEQDPAPSQTLE
  LEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDK
  KGKEHLAPTIRATVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQ
  SNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGII
  QGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFGAWFRAV
  ERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSHSKSCDQLRCGPYLSS
  GDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVA
  ATRTHKRSVSGLCNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEE
  EEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIA
  KGIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006266
• ORF Size: 2742 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006266.4
• RefSeq Size: 3656 bp
• RefSeq ORF: 2745 bp
• Locus ID: 5900
• UniProt ID: Q12967
• Cytogenetics: 9q34.13-q34.2
• Domains: RA, RasGEFN, RasGEF
• Protein Pathways: Colorectal cancer, Pancreatic cancer, Pathways in cancer
• Gene Summary: Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]