SFRS8 (SFSWAP) (NM_004592) Human Tagged ORF Clone

CAT#: RG216070

SFSWAP (tGFP-tagged) - Human splicing factor, suppressor of white-apricot homolog (Drosophila) (SFSWAP)

Product Images

Circular map for RG216070

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SFRS8
• Synonyms: SFRS8; SWAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG216070 representing NM_004592
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGGCGCGAGCGGGGGCCGCGCCAAACCCGAGAGGAAAAGCGGCGCGAAGGAGGAGGCCGGGCCAG
  GCGGTGCCGGCGGTGGGGGCAGCCGAGTGGAGCTCTTGGTTTTCGGCTATGCCTGCAAGCTGTTCCGGGA
  CGACGAGCGGGCCCTGGCTCAGGAACAGGGACAGCACCTCATCCCCTGGATGGGGGACCACAAGATCCTC
  ATCGACAGATATGATGGACGTGGTCACCTGCATGACCTTTCTGAGTACGATGCTGAGTATTCCACGTGGA
  ACAGAGATTATCAGCTGTCTGAAGAGGAGGCGCGAATAGAGGCCCTGTGTGATGAAGAGAGGTATTTAGC
  CTTGCATACGGACTTGCTTGAGGAGGAGGCAAGGCAAGAGGAAGAATACAAGCGATTGAGTGAAGCACTA
  GCAGAGGATGGGAGCTACAATGCCGTGGGGTTCACTTACGGTAGCGACTATTACGACCCGTCAGAGCCGA
  CGGAGGAGGAGGAGCCTTCCAAACAGAGAGAAAAAAATGAGGCCGAAAATTTAGAGGAAAATGAAGAGCC
  CTTCGTTGCCCCCTTAGGATTGAGCGTCCCGTCTGACGTGGAGTTGCCACCAACCGCTAAAATGCACGCC
  ATCATCGAGCGCACGGCCAGCTTCGTGTGCAGGCAGGGAGCACAGTTTGAGATCATGCTGAAGGCCAAGC
  AGGCCCGGAACTCCCAGTTTGACTTTCTGCGCTTCGACCACTACCTCAACCCCTACTATAAGTTCATCCA
  GAAAGCCATGAAAGAGGGACGCTACACTGTCCTGGCAGAAAACAAAAGTGACGAGAAAAAAAAATCAGGA
  GTCAGCTCTGACAATGAAGATGATGATGATGAAGAAGATGGGAATTACCTTCATCCCTCTCTCTTTGCCT
  CCAAGAAGTGTAACCGCCTTGAAGAGCTGATGAAGCCCTTGAAGGTAGTGGACCCAGATCATCCCCTCGC
  AGCACTTGTTCGTAAGGCACAGGCTGACAGTTCCACTCCCACCCCACACAACGCAGACGGTGCGCCTGTG
  CAGCCCTCCCAGGTGGAGTACACGGCAGACTCGACCGTGGCAGCCATGTATTACAGCTACTACATGCTAC
  CGGACGGCACTTACTGCCTGGCGCCGCCCCCTCCCGGAATCGACGTGACTACTTACTACAGCACCCTTCC
  TGCTGGCGTGACCGTGTCTAACTCCCCTGGAGTGACGACCACCGCCCCACCACCTCCTGGGACCACACCA
  CTACCGCCCCCAACCACAGCAGAGACTAGCAGCGGGGCCACCTCCACAACCACCACCACAAGTGCACTTG
  CCCCCGTGGCCGCCATCATCCCCCCGCCCCCCGACGTCCAGCCCGTGATTGACAAGCTGGCCGAGTATGT
  CGCCAGGAACGGCCTGAAGTTCGAGACCAGTGTTCGTGCCAAGAATGATCAAAGATTTGAGTTCCTGCAG
  CCGTGGCACCAGTATAATGCTTATTATGAGTTTAAGAAGCAGTTCTTCCTCCAGAAAGAAGGGGGCGATA
  GCATGCAGGCTGTGTCTGCACCAGAAGAGGCTCCCACAGACTCTGCTCCCGAGAAGCCAAGTGATGCTGG
  GGAGGATGGCGCGCCTGAAGACGCAGCCGAGGTGGGAGCACGGGCAGGCTCAGGCGGGAAGAAGGAGGCA
  TCGTCCAGTAAGACCGTCCCGGACGGGAAGCTGGTGAAAGCTTCCTTTGCTCCAATAAGCTTTGCAATCA
  AGGCCAAAGAAAATGATCTGCTTCCCCTGGAAAAAAATCGTGTTAAGCTAGATGATGACAGTGATGATGA
  TGAAGAAAGCAAAGAAGGCCAAGAAAGTTCTAGTAGTGCTGCAAACACTAACCCAGCAGTTGCCCCACCC
  TGTGTAGTTGTTGAGGAGAAGAAGCCTCAACTTACCCAGGAGGAGCTAGAAGCAAAGCAAGCAAAGCAAA
  AGCTGGAAGATCGCCTCGCAGCTGCTGCCCGGGAAAAGCTGGCCCAGGCGTCTAAGGAGTCAAAAGAGAA
  ACAGCTTCAAGCAGAACGTAAAAGGAAAGCGGCGTTATTTTTACAGACCCTCAAAAATCCTCTGCCGGAA
  GCAGAAGCTGGGAAAATTGAGGAGAGTCCTTTCAGTGTCGAGGAATCCAGCACTACGCCCTGCCCTCTAC
  TGACTGGAGGCAGGCCTCTGCCTACTTTAGAAGTTAAACCACCCGATAGGCCTTCGAGCAAAAGCAAAGA
  TCCACCGAGAGAAGAAGAGAAAGAAAAGAAAAAGAAAAAGCACAAAAAAAGATCTCGAACAAGATCACGT
  TCTCCCAAGTACCATTCGTCATCCAAGTCCAGGTCTAGATCACACTCAAAAGCAAAGCATTCTCTTCCCA
  GTGCCTATCGGACAGTGCGGCGGTCGAGGTCCCGCTCCCGGTCCCCTCGGAGGAGAGCCCACTCCCCTGA
  GAGACGGAGGGAAGAGAGGAGTGTGCCCACTGCCTACCGCGTGAGCCGCAGCCCTGGGGCCAGCAGGAAG
  CGGACCCGCTCCAGAAGTCCCCACGAGAAGAAGAAGAAGAGGCGGTCCCGGTCGCGGACCAAGTCCAAGG
  CCAGGTCTCAGTCGGTGTCACCCAGCAAGCAGGCAGCGCCCCGGCCCGCGGCCCCCGCGGCCCACTCGGC
  GCACTCAGCCAGCGTCTCCCCTGTGGAGAGTCGGGGCTCCAGCCAGGAGCGCTCCAGGGGAGTCTCTCAG
  GAAAAAGAAGCCCAGATCTCTTCAGCAATCGTTTCTTCCGTGCAGAGCAAAATCACTCAGGATCTCATGG
  CCAAAGTCAGAGCGATGCTTGCAGCTTCCAAAAACCTGCAAACCAGCGCTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG216070 representing NM_004592
  Red=Cloning site Green=Tags(s)
  
  MYGASGGRAKPERKSGAKEEAGPGGAGGGGSRVELLVFGYACKLFRDDERALAQEQGQHLIPWMGDHKIL
  IDRYDGRGHLHDLSEYDAEYSTWNRDYQLSEEEARIEALCDEERYLALHTDLLEEEARQEEEYKRLSEAL
  AEDGSYNAVGFTYGSDYYDPSEPTEEEEPSKQREKNEAENLEENEEPFVAPLGLSVPSDVELPPTAKMHA
  IIERTASFVCRQGAQFEIMLKAKQARNSQFDFLRFDHYLNPYYKFIQKAMKEGRYTVLAENKSDEKKKSG
  VSSDNEDDDDEEDGNYLHPSLFASKKCNRLEELMKPLKVVDPDHPLAALVRKAQADSSTPTPHNADGAPV
  QPSQVEYTADSTVAAMYYSYYMLPDGTYCLAPPPPGIDVTTYYSTLPAGVTVSNSPGVTTTAPPPPGTTP
  LPPPTTAETSSGATSTTTTTSALAPVAAIIPPPPDVQPVIDKLAEYVARNGLKFETSVRAKNDQRFEFLQ
  PWHQYNAYYEFKKQFFLQKEGGDSMQAVSAPEEAPTDSAPEKPSDAGEDGAPEDAAEVGARAGSGGKKEA
  SSSKTVPDGKLVKASFAPISFAIKAKENDLLPLEKNRVKLDDDSDDDEESKEGQESSSSAANTNPAVAPP
  CVVVEEKKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQTLKNPLPE
  AEAGKIEESPFSVEESSTTPCPLLTGGRPLPTLEVKPPDRPSSKSKDPPREEEKEKKKKKHKKRSRTRSR
  SPKYHSSSKSRSRSHSKAKHSLPSAYRTVRRSRSRSRSPRRRAHSPERRREERSVPTAYRVSRSPGASRK
  RTRSRSPHEKKKKRRSRSRTKSKARSQSVSPSKQAAPRPAAPAAHSAHSASVSPVESRGSSQERSRGVSQ
  EKEAQISSAIVSSVQSKITQDLMAKVRAMLAASKNLQTSAS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004592
• ORF Size: 2853 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004592.3, NP_004583.2
• RefSeq Size: 3258 bp
• RefSeq ORF: 2856 bp
• Locus ID: 6433
• UniProt ID: Q12872
• Cytogenetics: 12q24.33
• Domains: Surp
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a human homolog of Drosophila splicing regulatory protein. This gene autoregulates its expression by control of splicing of its first two introns. In addition, it also regulates the splicing of fibronectin and CD45 genes. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2012]