GMPPA (NM_205847) Human Tagged ORF Clone

CAT#: RG212726

GMPPA (tGFP-tagged) - Human GDP-mannose pyrophosphorylase A (GMPPA), transcript variant 2

Product Images

Circular map for RG212726

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GMPPA
• Synonyms: AAMR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212726 representing NM_205847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAAGCGGTGATCCTGATTGGAGGCCCTCAAAAGGGAACTCGCTTCAGACCTTTGTCTTTTGAGG
  TGCCCAAACCATTGTTTCCTGTGGCAGGGGTCCCTATGATCCAACACCATATTGAAGCCTGTGCCCAGGT
  CCCTGGAATGCAGGAGATTCTGCTCATTGGCTTCTACCAACCTGATGAGCCCCTCACCCAGTTCCTAGAA
  GCCGCCCAGCAGGAGTTTAACCTTCCAGTCAGGTACCTGCAGGAATTTGCCCCCCTAGGCACAGGGGGTG
  GTCTTTACCATTTTCGAGACCAGATCCTGGCTGGGAGCCCCGAGGCATTCTTCGTGCTCAATGCTGATGT
  CTGCTCCGACTTCCCCTTGAGTGCTATGTTGGAAGCCCACCGACGCCAGCGTCACCCTTTCTTACTCCTT
  GGCACTACGGCTAACAGGACGCAATCCCTCAACTACGGCTGCATCGTTGAGAATCCACAGACACACGAGG
  TATTGCACTATGTGGAGAAACCCAGCACATTTATCAGTGACATCATCAACTGCGGCATCTACCTCTTTTC
  TCCTGAAGCCTTGAAGCCTCTTCGGGATGTCTTCCAGCGTAATCAGCAGGATGGGCAATTGGAGGACTCA
  CCAGGCTTGTGGCCAGGGGCAGGTACCATCCGCCTAGAGCAGGATGTGTTTTCAGCCCTGGCAGGGCAGG
  GCCAGATATACGTGCATCTCACTGATGGTATCTGGAGTCAGATCAAGTCCGCAGGTTCAGCCCTCTACGC
  CTCCCGCCTCTACCTGAGCCGATACCAGGACACTCACCCAGAACGGCTGGCCAAGCACACCCCAGGGGGC
  CCATGGATCCGAGGGAATGTGTACATCCACCCGACCGCCAAGGTGGCCCCCTCGGCTGTGCTGGGCCCCA
  ACGTCTCCATCGGGAAGGGGGTGACCGTGGGTGAGGGTGTGCGGCTCCGGGAGAGCATCGTCCTCCATGG
  AGCCACTTTGCAGGAGCACACGTGTGTTCTGCATAGCATCGTGGGCTGGGGGAGCACCGTGGGACGCTGG
  GCCCGCGTGGAGGGTACCCCCAGTGACCCTAACCCCAACGATCCCCGAGCCCGCATGGACAGTGAGAGCC
  TCTTCAAGGACGGGAAGCTGCTGCCTGCTATCACCATCCTGGGCTGCCGAGTCCGGATCCCTGCCGAGGT
  GCTCATCCTGAACTCGATTGTTCTGCCACACAAGGAGCTGAGCCGAAGCTTCACCAACCAGATCATCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212726 representing NM_205847
  Red=Cloning site Green=Tags(s)
  
  MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMIQHHIEACAQVPGMQEILLIGFYQPDEPLTQFLE
  AAQQEFNLPVRYLQEFAPLGTGGGLYHFRDQILAGSPEAFFVLNADVCSDFPLSAMLEAHRRQRHPFLLL
  GTTANRTQSLNYGCIVENPQTHEVLHYVEKPSTFISDIINCGIYLFSPEALKPLRDVFQRNQQDGQLEDS
  PGLWPGAGTIRLEQDVFSALAGQGQIYVHLTDGIWSQIKSAGSALYASRLYLSRYQDTHPERLAKHTPGG
  PWIRGNVYIHPTAKVAPSAVLGPNVSIGKGVTVGEGVRLRESIVLHGATLQEHTCVLHSIVGWGSTVGRW
  ARVEGTPSDPNPNDPRARMDSESLFKDGKLLPAITILGCRVRIPAEVLILNSIVLPHKELSRSFTNQIIL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_205847
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205847.3
• RefSeq Size: 1880 bp
• RefSeq ORF: 1263 bp
• Locus ID: 29926
• UniProt ID: Q96IJ6
• Cytogenetics: 2q35
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: This gene is thought to encode a GDP-mannose pyrophosphorylase. This enzyme catalyzes the reaction which converts mannose-1-phosphate and GTP to GDP-mannose which is involved in the production of N-linked oligosaccharides. [provided by RefSeq, Jul 2008]