C2 (NM_000063) Human Tagged ORF Clone

CAT#: RG208097

C2 (tGFP-tagged) - Human complement component 2 (C2), transcript variant 1

Product Images

Circular map for RG208097

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C2
• Synonyms: ARMD14; CO2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208097 representing NM_000063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCACTGATGGTTCTTTTTTGCCTGCTGTTCCTGTACCCAGGTCTGGCAGACTCGGCTCCCTCCT
  GCCCTCAGAACGTGAATATCTCGGGTGGCACCTTCACCCTCAGCCATGGCTGGGCTCCTGGGAGCCTTCT
  CACCTACTCCTGCCCCCAGGGCCTGTACCCATCCCCAGCATCACGGCTGTGCAAGAGCAGCGGACAGTGG
  CAGACCCCAGGAGCCACCCGGTCTCTGTCTAAGGCGGTCTGCAAACCTGTGCGCTGTCCAGCCCCTGTCT
  CCTTTGAGAATGGCATTTATACCCCACGGCTGGGGTCCTATCCCGTGGGTGGCAATGTGAGCTTCGAGTG
  TGAGGATGGCTTCATATTGCGGGGCTCGCCTGTGCGTCAGTGTCGCCCCAACGGCATGTGGGATGGAGAA
  ACAGCTGTGTGTGATAATGGGGCTGGCCACTGCCCCAACCCAGGCATTTCACTGGGCGCAGTGCGGACAG
  GCTTCCGCTTTGGTCATGGGGACAAGGTCCGCTATCGCTGCTCCTCGAATCTTGTGCTCACGGGGTCTTC
  GGAGCGGGAGTGCCAGGGCAACGGGGTCTGGAGTGGAACGGAGCCCATCTGCCGCCAACCCTACTCTTAT
  GACTTCCCTGAGGACGTGGCCCCTGCCCTGGGCACTTCCTTCTCCCACATGCTTGGGGCCACCAATCCCA
  CCCAGAAGACAAAGGAAAGCCTGGGCCGTAAAATCCAAATCCAGCGCTCTGGTCATCTGAACCTCTACCT
  GCTCCTGGACTGTTCGCAGAGTGTGTCGGAAAATGACTTTCTCATCTTCAAGGAGAGCGCCTCCCTCATG
  GTGGACAGGATCTTCAGCTTTGAGATCAATGTGAGCGTTGCCATTATCACCTTTGCCTCAGAGCCCAAAG
  TCCTCATGTCTGTCCTGAACGACAACTCCCGGGATATGACTGAGGTGATCAGCAGCCTGGAAAATGCCAA
  CTATAAAGATCATGAAAATGGAACTGGGACTAACACCTATGCGGCCTTAAACAGTGTCTATCTCATGATG
  AACAACCAAATGCGACTCCTCGGCATGGAAACGATGGCCTGGCAGGAAATCCGACATGCCATCATCCTTC
  TGACAGATGGAAAGTCCAATATGGGTGGCTCTCCCAAGACAGCTGTTGACCATATCAGAGAGATCCTGAA
  CATCAACCAGAAGAGGAATGACTATCTGGACATCTATGCCATCGGGGTGGGCAAGCTGGATGTGGACTGG
  AGAGAACTGAATGAGCTAGGGTCCAAGAAGGATGGTGAGAGGCATGCCTTCATTCTGCAGGACACAAAGG
  CTCTGCACCAGGTCTTTGAACATATGCTGGATGTCTCCAAGCTCACAGACACCATCTGCGGGGTGGGGAA
  CATGTCAGCAAACGCCTCTGACCAGGAGAGGACACCCTGGCATGTCACTATTAAGCCCAAGAGCCAAGAG
  ACCTGCCGGGGGGCCCTCATCTCCGACCAATGGGTCCTGACAGCAGCTCATTGCTTCCGCGATGGCAACG
  ACCACTCCCTGTGGAGGGTCAATGTGGGAGACCCCAAATCCCAGTGGGGCAAAGAATTCCTTATTGAGAA
  GGCGGTGATCTCCCCAGGGTTTGATGTCTTTGCCAAAAAGAACCAGGGAATCCTGGAGTTCTATGGTGAT
  GACATAGCTCTGCTGAAGCTGGCCCAGAAAGTAAAGATGTCCACCCATGCCAGGCCCATCTGCCTTCCCT
  GCACGATGGAGGCCAATCTGGCTCTGCGGAGACCTCAAGGCAGCACCTGTAGGGACCATGAGAATGAACT
  GCTGAACAAACAGAGTGTTCCTGCTCATTTTGTCGCCTTGAATGGGAGCAAACTGAACATTAACCTTAAG
  ATGGGAGTGGAGTGGACAAGCTGTGCCGAGGTTGTCTCCCAAGAAAAAACCATGTTCCCCAACTTGACAG
  ATGTCAGGGAGGTGGTGACAGACCAGTTCCTATGCAGTGGGACCCAGGAGGATGAGAGTCCCTGCAAGGG
  AGAATCTGGGGGAGCAGTTTTCCTTGAGCGGAGATTCAGGTTTTTTCAGGTGGGTCTGGTGAGCTGGGGT
  CTTTACAACCCCTGCCTTGGCTCTGCTGACAAAAACTCCCGCAAAAGGGCCCCTCGTAGCAAGGTCCCGC
  CGCCACGAGACTTTCACATCAATCTCTTCCGCATGCAGCCCTGGCTGAGGCAGCACCTGGGGGATGTCCT
  GAATTTTTTACCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208097 representing NM_000063
  Red=Cloning site Green=Tags(s)
  
  MGPLMVLFCLLFLYPGLADSAPSCPQNVNISGGTFTLSHGWAPGSLLTYSCPQGLYPSPASRLCKSSGQW
  QTPGATRSLSKAVCKPVRCPAPVSFENGIYTPRLGSYPVGGNVSFECEDGFILRGSPVRQCRPNGMWDGE
  TAVCDNGAGHCPNPGISLGAVRTGFRFGHGDKVRYRCSSNLVLTGSSERECQGNGVWSGTEPICRQPYSY
  DFPEDVAPALGTSFSHMLGATNPTQKTKESLGRKIQIQRSGHLNLYLLLDCSQSVSENDFLIFKESASLM
  VDRIFSFEINVSVAIITFASEPKVLMSVLNDNSRDMTEVISSLENANYKDHENGTGTNTYAALNSVYLMM
  NNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGSPKTAVDHIREILNINQKRNDYLDIYAIGVGKLDVDW
  RELNELGSKKDGERHAFILQDTKALHQVFEHMLDVSKLTDTICGVGNMSANASDQERTPWHVTIKPKSQE
  TCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPKSQWGKEFLIEKAVISPGFDVFAKKNQGILEFYGD
  DIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQGSTCRDHENELLNKQSVPAHFVALNGSKLNINLK
  MGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCSGTQEDESPCKGESGGAVFLERRFRFFQVGLVSWG
  LYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQPWLRQHLGDVLNFLPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000063
• ORF Size: 2256 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000063.6
• RefSeq Size: 2609 bp
• RefSeq ORF: 2259 bp
• Locus ID: 717
• UniProt ID: P06681
• Cytogenetics: 6p21.33
• Domains: CCP, Tryp_SPc, VWA
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• Gene Summary: Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]