DP2 (TFDP2) (NM_006286) Human Tagged ORF Clone

CAT#: RG205845

TFDP2 (tGFP-tagged) - Human transcription factor Dp-2 (E2F dimerization partner 2) (TFDP2), transcript variant 2

Product Images

Circular map for RG205845

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DP2
• Synonyms: DP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205845 representing NM_006286
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTATAAGCACACCACAGAGACTAACCAGTTCAGGAAGTGTTCTGATTGGGAGTCCATATACCCCTG
  CACCAGCAATGGTTACTCAGACACACATAGCAGAAGCTACTGGCTGGGTCCCTGGTGATAGAAAACGGGC
  TAGAAAATTTATAGACTCTGATTTTTCAGAAAGTAAACGAAGCAAAAAAGGAGATAAAAATGGGAAAGGC
  TTGAGACACTTTTCAATGAAAGTGTGTGAGAAAGTTCAACGAAAAGGTACAACATCGTACAATGAAGTCG
  CTGATGAGCTGGTGTCAGAGTTCACCAATTCAAATAACCATTTGGCTGCTGATTCGCAGGCTTATGATCA
  GAAGAACATTAGGCGAAGAGTTTATGATGCTTTAAATGTGCTAATGGCAATGAACATAATTTCAAAGGAA
  AAAAAAGAAATCAAGTGGATTGGCCTGCCTACCAATTCTGCTCAGGAATGTCAGAATCTGGAGATAGAGA
  AGCAGAGGCGGATAGAACGGATAAAGCAGAAGCGGGCCCAGCTGCAAGAACTTCTCCTACAGCAAATCGC
  TTTCAAAAACCTGGTACAGAGAAATCGACAAAATGAGCAGCAAAACCAGGGCCCGCCGGCTCTGAACTCT
  ACCATTCAGCTGCCATTCATAATCATCAATACAAGCAGAAAAACAGTCATAGATTGCAGCATCTCCAGTG
  ACAAGTTTGAGTATCTTTTCAATTTTGACAACACCTTTGAGATCCATGATGACATAGAAGTACTAAAGCG
  GATGGGAATGTCGTTTGGCCTGGAGTCAGGCAAATGCTCTCTGGAGGATCTGAAACTTGCGAAATCCCTG
  GTGCCAAAGGCTTTAGAAGGTTATATCACAGATATCTCCACAGGACCTTCTTGGTTAAATCAGGGACTAC
  TTCTGAACTCTACCCAATCAGTTTCAAATTTAGACCTGACCACTGGTGCCACCTTACCCCAGTCAAGTGT
  AAACCAAGGGTTATGCTTGGATGCAGAAGTGGCCTTAGCAACTGGGCAGTTCCTGGCCCCAAACAGTCAC
  CAGTCCAGCAGTGCGGCCTCTCACTGCTCCGAGTCCCGAGGCGAGACCCCCTGTTCGTTCAATGATGAAG
  ATGAGGAAGATGATGAGGAGGATTCCTCCTCCCCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205845 representing NM_006286
  Red=Cloning site Green=Tags(s)
  
  MIISTPQRLTSSGSVLIGSPYTPAPAMVTQTHIAEATGWVPGDRKRARKFIDSDFSESKRSKKGDKNGKG
  LRHFSMKVCEKVQRKGTTSYNEVADELVSEFTNSNNHLAADSQAYDQKNIRRRVYDALNVLMAMNIISKE
  KKEIKWIGLPTNSAQECQNLEIEKQRRIERIKQKRAQLQELLLQQIAFKNLVQRNRQNEQQNQGPPALNS
  TIQLPFIIINTSRKTVIDCSISSDKFEYLFNFDNTFEIHDDIEVLKRMGMSFGLESGKCSLEDLKLAKSL
  VPKALEGYITDISTGPSWLNQGLLLNSTQSVSNLDLTTGATLPQSSVNQGLCLDAEVALATGQFLAPNSH
  QSSSAASHCSESRGETPCSFNDEDEEDDEEDSSSPE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006286
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006286.5
• RefSeq Size: 2320 bp
• RefSeq ORF: 1161 bp
• Locus ID: 7029
• UniProt ID: Q14188
• Cytogenetics: 3q23
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle
• Gene Summary: The gene is a member of the transcription factor DP family. The encoded protein forms heterodimers with the E2F transcription factors resulting in transcriptional activation of cell cycle regulated genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]