TARS2 (NM_025150) Human Tagged ORF Clone

CAT#: RG203395

TARS2 (tGFP-tagged) - Human threonyl-tRNA synthetase 2, mitochondrial (putative) (TARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RG203395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TARS2
• Synonyms: COXPD21; TARSL1; thrRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203395 representing NM_025150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGTATCAGAGGTGGCGGTGTCTCCGGCTCCAAGGTTTACAGGCTTGCAGGCTACACACGGCAG
  TTGTGTCGACCCCTCCACGCTGGTTGGCAGAGCGGCTTGGCCTTTTTGAGGAGCTGTGGGCTGCTCAGGT
  AAAGAGATTAGCAAGCATGGCACAGAAGGAACCCCGGACTATTAAGATATCACTTCCTGGAGGCCAGAAA
  ATTGATGCTGTGGCATGGAACACAACCCCCTACCAACTAGCCCGGCAGATCAGTTCAACACTGGCAGATA
  CTGCAGTGGCTGCTCAAGTGAATGGAGAACCTTATGATCTGGAGCGGCCCTTGGAGACAGATTCTGACCT
  CAGATTTCTGACATTCGATTCCCCAGAGGGGAAAGCAGTGTTCTGGCACTCCAGCACCCATGTCCTGGGG
  GCAGCAGCTGAACAATTCCTAGGTGCTGTTCTCTGCAGAGGTCCAAGTACAGAATATGGCTTTTACCATG
  ATTTCTTCCTGGGAAAGGAGAGGACAATCCGGGGCTCAGAGCTGCCTGTTTTGGAGCGGATTTGCCAGGA
  ACTTACAGCTGCTGCTCGACCCTTCCGGAGGCTAGAGGCTTCACGGGATCAGCTTCGCCAGTTGTTCAAG
  GATAACCCCTTTAAGCTTCACTTGATTGAGGAGAAAGTGACAGGTCCAACAGCAACAGTATATGGGTGTG
  GCACATTGGTTGACCTTTGCCAGGGCCCCCACCTTCGGCATACTGGACAGATTGGAGGACTGAAGCTGCT
  ATCGAACTCATCATCCTTATGGAGGTCTTCAGGGGCCCCAGAGACACTGCAGAGAGTGTCAGGGATTTCC
  TTCCCCACAACAGAATTGCTGAGGGTCTGGGAAGCATGGAGGGAGGAAGCAGAATTGCGGGACCACCGGC
  GCATTGGGAAGGAACAGGAGCTCTTCTTCTTCCATGAACTGAGCCCTGGGAGCTGCTTCTTCCTGCCACG
  AGGGACAAGGGTGTATAATGCACTAGTGGCGTTTATCAGGGCTGAGTATGCCCATCGTGGTTTCTCCGAG
  GTGAAAACTCCCACACTGTTTTCTACGAAGCTCTGGGAACAGTCAGGGCACTGGGAGCATTATCAGGAAG
  ACATGTTTGCCGTGCAGCCCCCAGGCTCTGACAGGCCTCCCAGCTCCCAGAGTGACGATTCTACCAGGCA
  TATCACAGATACACTCGCCCTCAAGCCTATGAACTGCCCTGCACACTGCCTGATGTTCGCCCACCGGCCC
  AGATCCTGGCGGGAACTGCCCCTGCGACTAGCTGACTTTGGGGCTCTACACCGGGCCGAAGCCTCTGGTG
  GTCTGGGGGGACTGACCCGACTGCGGTGCTTCCAGCAGGATGACGCTCACATCTTCTGTACAACAGATCA
  GCTGGAAGCAGAGATCCAAAGCTGTCTTGATTTCCTCCGTTCCGTCTATGCCGTTCTTGGCTTCTCCTTC
  CGCCTGGCACTGTCCACCCGGCCATCTGGCTTCCTGGGGGACCCTTGCCTTTGGGACCAGGCCGAACAGG
  TCCTTAAACAGGCCCTGAAGGAATTTGGAGAACCCTGGGACCTCAACTCTGGAGATGGTGCCTTCTATGG
  ACCTAAGATTGACGTGCACCTCCACGATGCCCTGGGCCGGCCACATCAGTGTGGGACAATTCAGCTTGAC
  TTCCAACTGCCCCTGAGATTTGACCTCCAGTATAAGGGGCAGGCGGGTGCCCTGGAGCGTCCAGTCCTCA
  TTCACCGAGCAGTGCTCGGTTCTGTGGAAAGACTGTTGGGAGTGCTGGCAGAAAGCTGCGGGGGGAAATG
  GCCACTGTGGCTGTCCCCGTTCCAGGTGGTGGTCATCCCTGTGGGGAGTGAGCAAGAGGAATACGCCAAA
  GAGGCACAGCAGAGCCTGCGGGCTGCAGGACTGGTCAGTGACCTGGATGCAGACTCTGGACTGACCCTCA
  GCCGGAGAATCCGCCGGGCCCAGCTTGCCCACTACAATTTTCAGTTTGTGGTTGGCCAGAAAGAGCAAAG
  TAAGAGAACAGTGAACATTCGGACTCGAGATAATCGTCGCCTTGGGGAGTGGGACTTGCCTGAGGCTGTG
  CAGCGACTGGTGGAGCTACAGAACACGAGGGTCCCAAATGCCGAAGAAATTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203395 representing NM_025150
  Red=Cloning site Green=Tags(s)
  
  MALYQRWRCLRLQGLQACRLHTAVVSTPPRWLAERLGLFEELWAAQVKRLASMAQKEPRTIKISLPGGQK
  IDAVAWNTTPYQLARQISSTLADTAVAAQVNGEPYDLERPLETDSDLRFLTFDSPEGKAVFWHSSTHVLG
  AAAEQFLGAVLCRGPSTEYGFYHDFFLGKERTIRGSELPVLERICQELTAAARPFRRLEASRDQLRQLFK
  DNPFKLHLIEEKVTGPTATVYGCGTLVDLCQGPHLRHTGQIGGLKLLSNSSSLWRSSGAPETLQRVSGIS
  FPTTELLRVWEAWREEAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRVYNALVAFIRAEYAHRGFSE
  VKTPTLFSTKLWEQSGHWEHYQEDMFAVQPPGSDRPPSSQSDDSTRHITDTLALKPMNCPAHCLMFAHRP
  RSWRELPLRLADFGALHRAEASGGLGGLTRLRCFQQDDAHIFCTTDQLEAEIQSCLDFLRSVYAVLGFSF
  RLALSTRPSGFLGDPCLWDQAEQVLKQALKEFGEPWDLNSGDGAFYGPKIDVHLHDALGRPHQCGTIQLD
  FQLPLRFDLQYKGQAGALERPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPFQVVVIPVGSEQEEYAK
  EAQQSLRAAGLVSDLDADSGLTLSRRIRRAQLAHYNFQFVVGQKEQSKRTVNIRTRDNRRLGEWDLPEAV
  QRLVELQNTRVPNAEEIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025150
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025150.5
• RefSeq Size: 2390 bp
• RefSeq ORF: 2157 bp
• Locus ID: 80222
• UniProt ID: Q9BW92
• Cytogenetics: 1q21.2
• Domains: tRNA-synt_2b, TGS, HGTP_anticodon
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: This gene encodes a member of the class-II aminoacyl-tRNA synthetase family. The encoded protein is a mitochondrial aminoacyl-tRNA synthetase. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 4. [provided by RefSeq, Dec 2012]