Myeloid leukemia factor 1 (MLF1) (NM_022443) Human Tagged ORF Clone

CAT#: RG202774

MLF1 (tGFP-tagged) - Human myeloid leukemia factor 1 (MLF1), transcript variant 1

Product Images

Circular map for RG202774

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Myeloid leukemia factor 1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202774 representing NM_022443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGGATGCTGAACAGCAGTTTTGAGGATGACCCCTTCTTCTCTGAGTCCATTCTTGCACACCGAG
  AAAATATGCGACAGATGATAAGAAGTTTTTCTGAACCCTTTGGAAGAGACTTGCTCAGTATCTCTGATGG
  TAGAGGGAGAGCTCATAATCGTAGAGGACATAATGATGGTGAAGATTCTTTGACTCATACAGATGTCAGC
  TCTTTCCAGACAATGGACCAAATGGTGTCAAATATGAGAAACTATATGCAGAAATTAGAAAGAAACTTCG
  GTCAACTTTCAGTGGATCCAAATGGACATTCATTTTGTTCTTCCTCAGTTATGACTTATTCCAAAATAGG
  AGATGAACCGCCAAAGGTTTTTCAGGCCTCAACTCAAACTCGTCGAGCTCCAGGAGGAATAAAGGAAACC
  AGGAAAGCAATGAGAGATTCTGACAGTGGACTAGAAAAAATGGCTATTGGTCATCATATCCATGACCGAG
  CTCATGTCATTAAAAAGTCAAAGAACAAGAAGACTGGAGATGAAGAGGTCAACCAGGAGTTCATCAATAT
  GAATGAAAGTGATGCTCATGCTTTTGATGAGGAGTGGCAAAGTGAGGTTTTGAAGTACAAACCAGGACGA
  CACAATCTAGGAAACACTAGAATGAGAAGTGTTGGCCATGAGAATCCTGGCTCCCGAGAACTTAAAAGAA
  GGGAGAAACCTCAACAAAGTCCAGCCATTGAACATGGAAGGAGATCAAATGTTTTGGGGGACAAACTCCA
  CATCAAAGGCTCATCTGTGAAAAGCAACAAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202774 representing NM_022443
  Red=Cloning site Green=Tags(s)
  
  MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVS
  SFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKET
  RKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGR
  HNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022443
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022443.5
• RefSeq Size: 1130 bp
• RefSeq ORF: 807 bp
• Locus ID: 4291
• UniProt ID: P58340
• Cytogenetics: 3q25.32
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]