MASA (ENOPH1) (NM_001292017) Human Tagged ORF Clone

CAT#: RC236269

ENOPH1 (myc-DDK-tagged) - Human enolase-phosphatase 1 (ENOPH1), transcript variant 2

Product Images

Circular map for RC236269

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MASA
• Synonyms: E1; MASA; MST145; mtnC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC236269 representing NM_001292017
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCAGGCCGTGGTAGATAATGTGTGCTGGCAGATGTCCCTGGATCGAAAGACCACTGCACTCAAAC
  AGCTGCAGGGCCACATGTGGAGGGCGGCATTCACAGCTGGGCGCATGAAAGCAGAGTTCTTTGCAGATGT
  AGTTCCAGCAGTCAGGAAGTGGAGAGAGGCCGGAATGAAGGTGTACATCTATTCCTCAGGGAGTGTGGAG
  GCACAGAAACTGTTATTCGGGCATTCTACGGAGGGAGATATTCTTGAGCTTGTTGATGGTCACTTTGATA
  CCAAGATTGGACACAAAGTAGAGAGTGAAAGTTACCGAAAGATTGCAGACAGCATTGGGTGCTCAACCAA
  CAACATTTTGTTTCTGACAGATGTTACTCGAGAGGCCAGTGCTGCTGAGGAAGCAGATGTGCACGTAGCT
  GTGGTGGTGAGACCAGGCAACGCAGGATTAACAGATGATGAGAAGACTTACTACAGCCTCATCACATCCT
  TCAGTGAACTATACCTGCCTTCCTCAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC236269 representing NM_001292017
  Red=Cloning site Green=Tags(s)
  
  MIQAVVDNVCWQMSLDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVE
  AQKLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREASAAEEADVHVA
  VVVRPGNAGLTDDEKTYYSLITSFSELYLPSST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001292017
• ORF Size: 519 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001292017.2
• RefSeq Size: 2162 bp
• RefSeq ORF: 522 bp
• Locus ID: 58478
• UniProt ID: Q9UHY7
• Cytogenetics: 4q21.22
• Protein Pathways: Cysteine and methionine metabolism
• MW: 19.6 kDa
• Gene Summary: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).[UniProtKB/Swiss-Prot Function]