ADAM8 (NM_001164489) Human Tagged ORF Clone

CAT#: RC228568

ADAM8 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 8 (ADAM8), transcript variant 2

Product Images

Circular map for RC228568

• 

  Western blot validation of overexpression lysate (Cat# LY431596) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228568 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAM8
• Synonyms: CD156; CD156a; MS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228568 representing NM_001164489
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCCTCGGGCTCTGGCTGCTGGGCGCGATGATGCTGCCTGCGATTGCCCCCAGCCGGCCCTGGG
  CCCTCATGGAGCAGTATGAGGTCGTGTTGCCGTGGCGTCTGCCAGGCCCCCGAGTCCGCCGAGCTCTGCC
  CTCCCACTTGGGCCTGCACCCAGAGAGGGTGAGCTACGTCCTTGGGGCCACAGGGCACAACTTCACCCTC
  CACCTGCGGAAGAACAGGGACCTGCTGGGCTCCGGCTACACAGAGACCTATACGGCTGCCAATGGCTCCG
  AGGTGACGGAGCAGCCTCGCGGGCAGGACCACTGCTTCTACCAGGGCCACGTAGAGGGGTACCCGGACTC
  AGCCGCCAGCCTCAGCACCTGTGCCGGCCTCAGGGGTTTCTTCCAGGTGGGGTCAGACCTGCACCTGATC
  GAGCCCCTGGATGAAGGTGGCGAGGGCGGACGGCACGCCGTGTACCAGGCTGAGCACCTGCTGCAGACGG
  CCGGGACCTGCGGGGTCAGCGACGACAGCCTGGGCAGCCTCCTGGGACCCCGGACGGCAGCCGTCTTCAG
  GCCTCGGCCCGGGGACTCTCTGCCATCCCGAGAGACCCGCTACGTGGAGCTGTATGTGGTCGTGGACAAT
  GCAGAGTTCCAGATGCTGGGGAGCGAAGCAGCCGTGCGTCATCGGGTGCTGGAGGTGGTGAATCACGTGG
  ACAAGCTATATCAGAAACTCAACTTCCGTGTGGTCCTGGTGGGCCTGGAGATTTGGAATAGTCAGGACAG
  GTTCCACGTCAGCCCCGACCCCAGTGTCACACTGGAGAACCTCCTGACCTGGCAGGCACGGCAACGGACA
  CGGCGGCACCTGCATGACAACGTACAGCTCATCACGGGTGTCGACTTCACCGGGACTACCGTGGGGTTTG
  CCAGGGTGTCCGCCATGTGCTCCCACAGCTCAGGGGCTGTGAACCAGGACCACAGCAAGAACCCCGTGGG
  CGTGGCCTGTACCATGGCCCATGAGATGGGCCACAACCTGGGCATGGACCATGATGAGAACGTCCAGGGC
  TGCCGCTGCCAGGAACGCTTCGAGGCCGGCCGCTGCATCATGGCGGGCAGCATTGGCTCCAGTTTCCCCA
  GGATGTTCAGTGACTGCAGCCAGGCCTACCTGGAGAGCTTTTTGGAGCGGCCGCAGTCGGTGTGCCTCGC
  CAACGCCCCTGACCTCAGCCACCTGGTGGGCGGCCCCGTGTGTGGGAACCTGTTTGTGGAGCGTGGGGAG
  CAGTGCGACTGCGGCCCCCCCGAGGACTGCCGGAACCGCTGCTGCAACTCTACCACCTGCCAGCTGGCTG
  AGGGGGCCCAGTGTGCGCACGGTACCTGCTGCCAGGAGTGCAAGGTGAAGCCGGCTGGTGAGCTGTGCCG
  TCCCAAGAAGGACATGTGTGACCTCGAGGAGTTCTGTGACGGCCGGCACCCTGAGTGCCCGGAAGACGCC
  TTCCAGGAGAACGGCACGCCCTGCTCCGGGGGCTACTGCTACAACGGGGCCTGTCCCACACTGGCCCAGC
  AGTGCCAGGCCTTCTGGGGGCCAGGTGGGCAGGCTGCCGAGGAGTCCTGCTTCTCCTATGACATCCTACC
  AGGCTGCAAGGCCAGCCGGTACAGGGCTGACATGTGTGGCGTTCTGCAGTGCAAGGGTGGGCAGCAGCCC
  CTGGGGCGTGCCATCTGCATCGTGGATGTGTGCCACGCGCTCACCACAGAGGATGGCACTGCGTATGAAC
  CAGTGCCCGAGGGCACCCGGTGTGGACCAGAGAAGGTTTGCTGGAAAGGACGTTGCCAGGACTTACACGT
  TTACAGATCCAGCAACTGCTCTGCCCAGTGCCACAACCATGGGGTGTGCAACCACAAGCAGGAGTGCCAC
  TGCCACGCGGGCTGGGCCCCGCCCCACTGCGCGAAGCTGCTGACTGAGGTGCACGCAGGCTGCCAGCCGC
  GTGCCGGCCAAGGGCGGGGCTCCAGCCCCATCCAGGGGCCCCCAAGAGCTGGTCCCCACCACCCACCCGG
  GCCAGCCCGCCCGACACCCGGCCTCCTCGGTGGCTCTGAAGAGGCCGCCCCCTGCTCCTCCGGTCACTGT
  GTCCAGCCCACCCTTCCCAGTTCCTGTCTACACCCGGCAGGCACCAAAGCAGGTCATCAAGCCAACGTTC
  GCACCCCCAGTGCCCCCAGTCAAACCCGGGGCTGGTGCGGCCAACCCTGGTCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228568 representing NM_001164489
  Red=Cloning site Green=Tags(s)
  
  MRGLGLWLLGAMMLPAIAPSRPWALMEQYEVVLPWRLPGPRVRRALPSHLGLHPERVSYVLGATGHNFTL
  HLRKNRDLLGSGYTETYTAANGSEVTEQPRGQDHCFYQGHVEGYPDSAASLSTCAGLRGFFQVGSDLHLI
  EPLDEGGEGGRHAVYQAEHLLQTAGTCGVSDDSLGSLLGPRTAAVFRPRPGDSLPSRETRYVELYVVVDN
  AEFQMLGSEAAVRHRVLEVVNHVDKLYQKLNFRVVLVGLEIWNSQDRFHVSPDPSVTLENLLTWQARQRT
  RRHLHDNVQLITGVDFTGTTVGFARVSAMCSHSSGAVNQDHSKNPVGVACTMAHEMGHNLGMDHDENVQG
  CRCQERFEAGRCIMAGSIGSSFPRMFSDCSQAYLESFLERPQSVCLANAPDLSHLVGGPVCGNLFVERGE
  QCDCGPPEDCRNRCCNSTTCQLAEGAQCAHGTCCQECKVKPAGELCRPKKDMCDLEEFCDGRHPECPEDA
  FQENGTPCSGGYCYNGACPTLAQQCQAFWGPGGQAAEESCFSYDILPGCKASRYRADMCGVLQCKGGQQP
  LGRAICIVDVCHALTTEDGTAYEPVPEGTRCGPEKVCWKGRCQDLHVYRSSNCSAQCHNHGVCNHKQECH
  CHAGWAPPHCAKLLTEVHAGCQPRAGQGRGSSPIQGPPRAGPHHPPGPARPTPGLLGGSEEAAPCSSGHC
  VQPTLPSSCLHPAGTKAGHQANVRTPSAPSQTRGWCGQPWSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164489
• ORF Size: 2226 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164489.2
• RefSeq ORF: 2229 bp
• Locus ID: 101
• UniProt ID: P78325
• Cytogenetics: 10q26.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 80.1 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration, and it is thought to be a target for allergic respiratory diseases, including asthma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2009]