GGT5 (NM_001099782) Human Tagged ORF Clone

CAT#: RC218872

GGT5 (Myc-DDK-tagged)-Human gamma-glutamyltransferase 5 (GGT5), transcript variant 3

Product Images

Circular map for RC218872

• 

  Western blot validation of overexpression lysate (Cat# LY420532) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218872 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGT5
• Synonyms: GGL; GGT-REL; GGT 5; GGTLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218872 representing NM_001099782
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGGCTACGGGGCCACGGTCAGCCTAGTCCTGCTGGGTCTGGGGCTGGCGCTGGCTGTCATTG
  TGCTGGCTGTGGTCCTCTCTCGACACCAGGCCCCATGTGGCCCCCAGGCCTTTGCCCACGCTGCTGTTGC
  CGCCGACTCCAAGGTCTGCTCGGATATTGGACGAGCCATCCTCCAGCAGCAGGGCTCACCCGTGGATGCC
  ACCATCGCGGCTCTGGTCTGCACCAGCGTCGTCAACCCTCAGAGCATGGGCCTGGGCGGAGGGGTCATCT
  TCACCATCTACAATGTGACAACAGGGGCCCAGTGGATCGGGGTGCCCGGGGAGCTCCGTGGCTATGCCGA
  GGCCCACCGCCGCCATGGCCGCCTGCCCTGGGCGCAGCTGTTCCAGCCCACCATCGCGCTGCTCCGAGGG
  GGGCATGTGGTGGCCCCTGTCCTCAGCCGTTTCCTGCACAACAGCATCCTGCGGCCTTCCTTGCAGGCGT
  CAACCCTGCGCCAGCTCTTCTTCAACGGGACAGAACCCCTGAGGCCTCAGGACCCACTCCCATGGCCTGC
  ACTGGCCACCACCCTGGAGACCGTGGCCACAGAGGGCGTGGAGGTCTTCTACACGGGGAGGCTGGGCCAG
  ATGCTGGTGGAGGACATTGCCAAGGAAGGGAGCCAGCTGACGCTGCAGGACCTGGCCAAGTTCCAGCCCG
  AGGTGGTGGATGCCCTGGAGGTGCCCCTGGGGGACTATACCCTGTACTCACCACCGCCGCCTGCAGGGGG
  TGCCATTCTCAGCTTTATCCTCAACGTGCTAAGAGGGTTCAACTTCTCAACAGAGTCTATGGCCAGGCCT
  GAAGGGAGGGTGAACGTGTACCACCACCTTGTAGAGACGCTCAAGTTTGCCAAGGGGCAGAGGTGGAGGC
  TGGGGGACCCTCGAAGCCACCCGAAGCTCCAGAATGCCTCCCGGGACCTGCTGGGGGAGACCCTGGCCCA
  GCTCATCCGCCAACAGATCGATGGCCGGGGGGACCACCAGCTCAGCCACTACAGCTTGGCCGAGGCCTGG
  GGCCACGGGACAGGCACGTCCCATGTGTCTGTGCTGGGGGAGGATGGCAGCGCCGTGGCTGCCACCAGCA
  CCATCAACACACCCTTTGGAGCGATGGTGTATTCACCACGGACAGGCATCATCCTCAACAACGAGCTCCT
  GGACTTATGCGAGCGATGCCCCCGGGGTTCCGGCACCACCCCCTCACCTGTGAGTGGAGACAGGGTGGGT
  GGAGCTCCCGGAAGGTGCTGGCCCCCAGTTCCAGGCGAGCGTTCCCCATCCTCCATGGTGCCCTCCATCT
  TGATCAACAAAGCCCAGGGGTCGAAGCTAGTGATTGGCGGGGCTGGCGGGGAGCTCATCATCTCTGCTGT
  GGCCCAGGCCATCATGAGCAAGCTGTGGCTTGGCTTTGACCTGAGAGCGGCCATTGCAGCCCCCATCCTG
  CATGTCAACAGCAAGGGCTGTGTGGAGTACGAGCCCAACTTCAGCCAGGAGGTGCAGAGGGGACTCCAAG
  ACCGTGGCCAGAACCAGACCCAGAGGCCCTTCTTCCTGAACGTGGTCCAGGCTGTGTCCCAGGAGGGGGC
  CTGTGTGTACGCCGTCTCGGACCTGAGGAAGAGTGGGGAGGCCGCAGGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218872 representing NM_001099782
  Red=Cloning site Green=Tags(s)
  
  MARGYGATVSLVLLGLGLALAVIVLAVVLSRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVDA
  TIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALLRG
  GHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGRLGQ
  MLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTESMARP
  EGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYSLAEAW
  GHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVG
  GAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPIL
  HVNSKGCVEYEPNFSQEVQRGLQDRGQNQTQRPFFLNVVQAVSQEGACVYAVSDLRKSGEAAGY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099782
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099782.2, NP_001093252.1
• RefSeq Size: 2401 bp
• RefSeq ORF: 1665 bp
• Locus ID: 2687
• UniProt ID: P36269
• Cytogenetics: 22q11.23
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• MW: 58.8 kDa
• Gene Summary: This gene is a member of the gamma-glutamyl transpeptidase gene family, and some reports indicate that it is capable of cleaving the gamma-glutamyl moiety of glutathione. The protein encoded by this gene is synthesized as a single, catalytically-inactive polypeptide, that is processed post-transcriptionally to form a heavy and light subunit, with the catalytic activity contained within the small subunit. The encoded enzyme is able to convert leukotriene C4 to leukotriene D4, but appears to have distinct substrate specificity compared to gamma-glutamyl transpeptidase. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]