Laminin (LAMC3) (NM_006059) Human Tagged ORF Clone

CAT#: RC218146

LAMC3 (Myc-DDK-tagged)-Human laminin, gamma 3 (LAMC3)

Product Images

Circular map for RC218146

• 

  Western blot validation of overexpression lysate (Cat# LY416892) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218146 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LAMC3 protein (Cat# TP318146). The protein was produced from HEK293T cells transfected with LAMC3 cDNA clone (Cat# RC218146) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Laminin
• Synonyms: OCCM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218146 representing NM_006059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCGCTTCTGCTGGGGCTGGCGCTGCTGGCACCGCGGGCGGCCGGCGCGGGCATGGGCG
  CGTGCTATGACGGCGCAGGGCGCCCGCAGCGCTGCCTGCCGGTGTTCGAGAACGCGGCGTTTGGGCGGCT
  CGCCCAGGCCTCGCACACGTGCGGCAGCCCGCCCGAGGACTTCTGTCCCCACGTGGGCGCCGCGGGCGCG
  GGGGCTCATTGCCAGCGCTGCGACGCCGCCGACCCCCAGCGCCACCACAACGCCTCCTACCTCACCGACT
  TCCACAGCCAGGACGAGAGCACCTGGTGGCAGAGCCCGTCCATGGCCTTCGGCGTGCAGTACCCCACCTC
  GGTCAACATCACCCTCCGCCTAGGGAAGGCTTATGAGATCACGTATGTGAGGCTGAAGTTCCACACCAGT
  CGCCCTGAGAGCTTTGCCATCTACAAGCGCAGCCGCGCCGACGGCCCATGGGAGCCCTACCAGTTCTACA
  GCGCCTCCTGCCAGAAGACCTACGGCCGGCCCGAGGGCCAGTACCTGCGCCCCGGCGAGGACGAGCGCGT
  GGCCTTCTGCACCTCTGAGTTCAGCGACATCTCCCCGCTGAGTGGCGGCAACGTGGCCTTCTCCACCCTG
  GAGGGCCGGCCCAGCGCCTACAACTTCGAGGAGAGCCCTGGGCTGCAGGAGTGGGTCACCAGCACCGAAC
  TCCTCATCTCTCTAGACCGGCTCAACACGTTTGGGGACGACATCTTCAAGGACCCCAAGGTGCTCCAGTC
  CTACTATTATGCCGTGTCCGACTTCTCTGTGGGCGGCAGGTGCAAGTGCAACGGGCATGCCAGCGAGTGC
  GGCCCCGACGTGGCAGGCCAGTTGGCCTGCCGGTGCCAGCACAACACCACCGGCACAGACTGTGAGCGCT
  GCCTGCCCTTCTTCCAGGACCGCCCGTGGGCCCGGGGCACCGCCGAGGCTGCCCACGAGTGTCTGCCCTG
  CAACTGCAGTGGCCGCTCCGAGGAATGCACGTTTGATCGGGAGCTCTTCCGCAGCACAGGCCACGGCGGG
  CGCTGTCACCACTGCCGTGACCACACAGCTGGGCCACACTGTGAGCGCTGTCAGGAGAATTTCTATCACT
  GGGACCCGCGGATGCCATGCCAGCCCTGTGACTGCCAGTCGGCAGGCTCCCTACACCTCCAGTGCGATGA
  CACAGGCACCTGCGCCTGCAAGCCCACGGTGACTGGCTGGAAGTGTGACCGCTGTCTGCCCGGGTTCCAC
  TCGCTCAGTGAGGGAGGCTGCAGACCCTGCACTTGCAATCCCGCTGGCAGCCTGGACACCTGTGACCCCC
  GCAGTGGGCGCTGCCCCTGCAAAGAGAATGTGGAAGGCAACCTATGTGACAGATGTCGCCCGGGGACCTT
  TAACCTGCAGCCCCACAATCCAGCTGGCTGCAGCAGCTGTTTCTGCTATGGCCACTCCAAGGTGTGCGCG
  TCCACTGCCCAGTTCCAGGTGCATCACATCCTCAGCGATTTCCACCAGGGAGCCGAAGGCTGGTGGGCCA
  GAAGTGTGGGGGGCTCTGAGCACCCCCCACAATGGAGCCCAAATGGGGTCCTCCTGAGCCCAGAAGACGA
  GGAGGAGCTCACAGCACCAGAGAAGTTCCTGGGAGACCAGCGGTTCAGCTATGGGCAGCCCCTCATACTG
  ACCTTCCGGGTGCCCCCCGGGGACTCCCCACTCCCTGTACAGCTGAGGCTGGAAGGGACAGGCTTGGCCC
  TGTCCCTGAGGCACTCTAGCCTGTCTGGCCCCCAGGATGCCGGGCATCCCAGGGAGGTAGAGCTCAGGTT
  CCACCTGCAGGAGACCTCCGAGGACGTGGCCCCTCCACTGCCCCCCTTCCACTTCCAGCGGCTCCTCGCC
  AACCTGACCAGCCTCCGCCTCCGCGTCAGTCCCGGCCCCAGCCCTGCCGGTCCAGTGTTCCTGACTGAGG
  TCCGGCTCACATCCGCCCGGCCAGGGCTTTCCCCGCCAGCCTCCTGGGTGGAGATTTGTTCATGTCCCAC
  TGGCTACACGGGCCAGTTCTGTGAATCCTGTGCTCCGGGATACAAGAGGGAGATGCCACAGGGGGGTCCC
  TATGCCAGCTGTGTCCCCTGCACCTGTAACCAGCATGGCACCTGTGACCCCAACACAGGGATCTGTGTCT
  GCAGCCACCATACCGAGGGCCCATCCTGTGAACGCTGTTTGCCAGGTTTCTATGGCAACCCTTTCGCGGG
  CCAAGCCGACGACTGCCAGCCCTGTCCCTGCCCTGGCCAGTCGGCCTGTACGACCATCCCAGAGAGCCGG
  GAGGTGGTGTGTACCCACTGCCCCCCGGGCCAGAGAGGGCGGCGCTGTGAGGTCTGTGATGATGGCTTTT
  TTGGGGACCCGCTGGGGCTCTTTGGGCACCCCCAGCCCTGCCACCAGTGCCAGTGTAGCGGGAACGTGGA
  CCCCAATGCCGTGGGCAACTGTGACCCCCTGTCTGGCCACTGCCTGCGCTGCCTGCACAACACCACGGGT
  GACCACTGTGAGCACTGTCAGGAAGGCTTCTACGGGAGCGCCCTGGCCCCTCGACCCGCAGACAAATGCA
  TGCCTTGCAGCTGTCACCCACAGGGCTCGGTCAGTGAGCAGATGCCCTGCGACCCAGTGACAGGCCAATG
  CTCCTGCCTGCCTCATGTGACTGCACGGGACTGCAGCCGCTGCTACCCTGGCTTCTTCGACCTCCAGCCT
  GGGAGGGGCTGCCGGAGCTGCAAGTGTCACCCACTGGGCTCCCAGGAGGACCAGTGCCATCCCAAGACTG
  GACAGTGCACCTGCCGCCCAGGTGTCACAGGCCAGGCCTGTGACAGGTGCCAGCTGGGTTTCTTCGGCTT
  CTCCATCAAGGGCTGCCGGGCCTGCAGGTGCTCCCCACTGGGCGCTGCCTCGGCCCAGTGCCACGAGAAC
  GGCACATGCGTGTGCAGGCCTGGCTTCGAGGGCTACAAATGTGACCGCTGCCACGACAACTTCTTCCTCA
  CGGCAGACGGCACACACTGCCAGCAATGTCCGTCCTGCTACGCCCTGGTGAAGGAGGAGGCAGCCAAGCT
  GAAGGCCAGACTGACTTTGACGGAGGGGTGGCTCCAAGGGTCCGACTGTGGCAGTCCCTGGGGACCACTA
  GACATTCTGCTGGGAGAGGCCCCAAGGGGGGACGTCTACCAGGGCCATCACCTGCTTCCAGGGGCTCGGG
  AAGCCTTCCTGGAGCAGATGATGAGCCTCGAGGGTGCTGTCAAGGCCGCCCGGGAGCAGCTGCAGAGGCT
  GAACAAGGGTGCCCGCTGTGCCCAGGCCGGATCCCAGAAGACCTGCACCCAGCTGGCAGACCTGGAGGCA
  GTGCTGGAGTCCTCGGAAGAGGAGATTCTGCATGCAGCTGCCATTCTCGCGTCTCTGGAGATTCCTCAGG
  AAGGTCCCAGTCAGCCGACCAAATGGAGCCACCTGGCCACAGAGGCCCGTGCCCTCGCCAGGAGCCACAG
  AGACACCGCCACCAAGATCGCAGCCACTGCTTGGAGGGCCCTGCTCGCCTCCAACACCAGCTACGCGCTT
  CTCTGGAATCTGCTGGAGGGAAGGGTGGCCCTAGAGACCCAGCGGGACCTGGAGGACAGGTACCAGGAGG
  TCCAGGCGGCCCAGAAAGCACTGAGGACGGCTGTGGCAGAGGTGCTGCCTGAAGCGGAAAGCGTGTTGGC
  CACCGTGCAGCAAGTTGGCGCAGATACAGCCCCGTACCTGGCCTTGCTGGCTTCCCCGGGAGCTCTGCCT
  CAGAAGTCCCGGGCTGAAGACCTGGGCCTGAAGGCGAAGGCCCTGGAGAAGACAGTTGCATCATGGCAGC
  ACATGGCCACTGAGGCTGCCCGAACCCTCCAGACTGCTGCCCAGGCGACGCTACGGCAAACAGAACCCCT
  CACAAAGCTGCACCAGGAGGCCAGAGCCGCCCTGACCCAGGCTTCCTCATCTGTCCAGGCTGCGACAGTG
  ACTGTCATGGGAGCCAGGACTCTGCTGGCTGATCTGGAAGGAATGAAGCTGCAGTTTCCCCGGCCCAAGG
  ACCAGGCGGCATTGCAGAGGAAGGCAGACTCCGTCAGTGACAGACTCCTTGCAGACACGAGAAAGAAGAC
  CAAGCAGGCGGAGAGGATGCTGGGAAACGCGGCCCCTCTTTCCTCCAGTGCCAAGAAGAAGGGCAGAGAA
  GCAGAGGTGTTGGCCAAGGACAGTGCCAAGCTTGCCAAGGCCTTGCTGAGGGAGCGGAAACAGGCGCACC
  GCCGTGCCAGCAGGCTCACCAGCCAGACGCAAGCCACGCTCCAACAGGCGTCCCAGCAGGTGCTGGCGTC
  TGAAGCACGCAGACAGGAGCTGGAGGAAGCTGAGCGGGTGGGTGCTGGGCTGAGCGAGATGGAGCAGCAG
  ATCCGGGAATCGCGTATCTCACTGGAGAAGGACATCGAGACCTTGTCAGAGCTGCTTGCCAGGCTGGGGT
  CGCTGGACACCCATCAAGCCCCAGCCCAGGCCCTGAACGAGACTCAGTGGGCACTAGAACGCCTGAGGCT
  GCAGCTGGGCTCCCCGGGGTCCTTGCAGAGGAAACTCAGTCTGCTGGAGCAGGAATCCCAGCAGCAGGAG
  CTGCAGATCCAGGGCTTCGAGAGTGACCTCGCCGAGATCCGCGCCGACAAACAGAACCTGGAGGCCATTC
  TGCACAGCCTGCCCGAGAACTGTGCCAGCTGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218146 representing NM_006059
  Red=Cloning site Green=Tags(s)
  
  MAAAALLLGLALLAPRAAGAGMGACYDGAGRPQRCLPVFENAAFGRLAQASHTCGSPPEDFCPHVGAAGA
  GAHCQRCDAADPQRHHNASYLTDFHSQDESTWWQSPSMAFGVQYPTSVNITLRLGKAYEITYVRLKFHTS
  RPESFAIYKRSRADGPWEPYQFYSASCQKTYGRPEGQYLRPGEDERVAFCTSEFSDISPLSGGNVAFSTL
  EGRPSAYNFEESPGLQEWVTSTELLISLDRLNTFGDDIFKDPKVLQSYYYAVSDFSVGGRCKCNGHASEC
  GPDVAGQLACRCQHNTTGTDCERCLPFFQDRPWARGTAEAAHECLPCNCSGRSEECTFDRELFRSTGHGG
  RCHHCRDHTAGPHCERCQENFYHWDPRMPCQPCDCQSAGSLHLQCDDTGTCACKPTVTGWKCDRCLPGFH
  SLSEGGCRPCTCNPAGSLDTCDPRSGRCPCKENVEGNLCDRCRPGTFNLQPHNPAGCSSCFCYGHSKVCA
  STAQFQVHHILSDFHQGAEGWWARSVGGSEHPPQWSPNGVLLSPEDEEELTAPEKFLGDQRFSYGQPLIL
  TFRVPPGDSPLPVQLRLEGTGLALSLRHSSLSGPQDAGHPREVELRFHLQETSEDVAPPLPPFHFQRLLA
  NLTSLRLRVSPGPSPAGPVFLTEVRLTSARPGLSPPASWVEICSCPTGYTGQFCESCAPGYKREMPQGGP
  YASCVPCTCNQHGTCDPNTGICVCSHHTEGPSCERCLPGFYGNPFAGQADDCQPCPCPGQSACTTIPESR
  EVVCTHCPPGQRGRRCEVCDDGFFGDPLGLFGHPQPCHQCQCSGNVDPNAVGNCDPLSGHCLRCLHNTTG
  DHCEHCQEGFYGSALAPRPADKCMPCSCHPQGSVSEQMPCDPVTGQCSCLPHVTARDCSRCYPGFFDLQP
  GRGCRSCKCHPLGSQEDQCHPKTGQCTCRPGVTGQACDRCQLGFFGFSIKGCRACRCSPLGAASAQCHEN
  GTCVCRPGFEGYKCDRCHDNFFLTADGTHCQQCPSCYALVKEEAAKLKARLTLTEGWLQGSDCGSPWGPL
  DILLGEAPRGDVYQGHHLLPGAREAFLEQMMSLEGAVKAAREQLQRLNKGARCAQAGSQKTCTQLADLEA
  VLESSEEEILHAAAILASLEIPQEGPSQPTKWSHLATEARALARSHRDTATKIAATAWRALLASNTSYAL
  LWNLLEGRVALETQRDLEDRYQEVQAAQKALRTAVAEVLPEAESVLATVQQVGADTAPYLALLASPGALP
  QKSRAEDLGLKAKALEKTVASWQHMATEAARTLQTAAQATLRQTEPLTKLHQEARAALTQASSSVQAATV
  TVMGARTLLADLEGMKLQFPRPKDQAALQRKADSVSDRLLADTRKKTKQAERMLGNAAPLSSSAKKKGRE
  AEVLAKDSAKLAKALLRERKQAHRRASRLTSQTQATLQQASQQVLASEARRQELEEAERVGAGLSEMEQQ
  IRESRISLEKDIETLSELLARLGSLDTHQAPAQALNETQWALERLRLQLGSPGSLQRKLSLLEQESQQQE
  LQIQGFESDLAEIRADKQNLEAILHSLPENCASWQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006059
• ORF Size: 4725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006059.4
• RefSeq Size: 5100 bp
• RefSeq ORF: 4728 bp
• Locus ID: 10319
• UniProt ID: Q9Y6N6
• Cytogenetics: 9q34.12
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• MW: 171.23 kDa
• Gene Summary: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 3. The gamma 3 chain is most similar to the gamma 1 chain, and contains all the 6 domains expected of the gamma chain. It is a component of laminin 12. The gamma 3 chain is broadly expressed in skin, heart, lung, and the reproductive tracts. In skin, it is seen within the basement membrane of the dermal-epidermal junction at points of nerve penetration. Gamma 3 is also a prominent element of the apical surface of ciliated epithelial cells of lung, oviduct, epididymis, ductus deferens, and seminiferous tubules. The distribution of gamma 3-containing laminins along ciliated epithelial surfaces suggests that the apical laminins are important in the morphogenesis and structural stability of the ciliated processes of these cells. [provided by RefSeq, Aug 2011]