NLRP14 (NM_176822) Human Tagged ORF Clone

CAT#: RC215050

  • TrueORF®

NLRP14 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 14 (NLRP14)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_176822" in other vectors (6)

Reconstitution Protocol

USD 951.00

5 Days*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit Polyclonal NALP14 Antibody
    • 100 ug

USD 570.00

Other products for "NLRP14"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NLRP14
Synonyms CLR11.2; GC-LRR; NALP14; NOD5; PAN8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215050 representing NM_176822
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGATTCATCATCATCTTCTTTCTTTCCTGATTTTGGGCTGCTATTGTATTTGGAGGAGCTAAACA
AAGAGGAATTAAATACATTCAAGTTATTCCTAAAGGAGACCATGGAACCTGAGCATGGCCTGACACCCTG
GAATGAAGTGAAGAAGGCCAGGCGGGAGGACCTGGCCAATTTGATGAAGAAATATTATCCAGGAGAGAAA
GCCTGGAGTGTGTCTCTCAAAATCTTTGGCAAGATGAACCTGAAGGATCTGTGTGAGAGAGCGAAAGAAG
AGATCAACTGGTCGGCCCAGACTATAGGACCAGATGATGCCAAGGCTGGAGAGACACAAGAAGATCAGGA
GGCAGTGCTGGGTGATGGAACAGAATACAGAAATAGAATAAAGGAAAAATTTTGCATCACTTGGGACAAG
AAGTCTTTGGCTGGAAAGCCTGAAGATTTCCATCATGGAATTGCAGAGAAAGATAGAAAACTGTTGGAAC
ACTTGTTCGATGTGGATGTCAAAACCGGTGCACAGCCACAGATCGTGGTGCTTCAGGGAGCTGCTGGAGT
TGGGAAAACAACCTTGGTGAGAAAGGCAATGTTAGATTGGGCAGAGGGCAGTCTCTACCAGCAGAGGTTT
AAGTATGTTTTTTATCTCAATGGGAGAGAAATTAACCAGCTGAAAGAGAGAAGCTTTGCTCAATTGATAT
CAAAGGACTGGCCCAGCACAGAAGGCCCCATTGAAGAAATCATGTACCAGCCAAGTAGCCTCTTGTTTAT
TATTGACAGTTTCGATGAACTGAACTTTGCCTTTGAAGAACCTGAGTTTGCACTGTGCGAAGACTGGACC
CAAGAACACCCAGTGTCCTTCCTCATGAGTAGTTTGCTGAGGAAAGTGATGCTCCCTGAGGCATCCTTAT
TGGTGACAACAAGACTCACAACTTCTAAGAGACTAAAGCAGTTGTTGAAGAATCACCATTATGTAGAGCT
ACTAGGAATGTCTGAGGATGCAAGAGAGGAGTATATTTACCAGTTTTTTGAAGATAAGAGGTGGGCCATG
AAAGTATTCAGTTCACTAAAAAGCAATGAGATGCTGTTTAGCATGTGCCAAGTCCCCCTAGTGTGCTGGG
CCGCTTGTACTTGTCTGAAGCAGCAAATGGAGAAGGGTGGTGATGTCACATTGACCTGCCAAACAACCAC
AGCTCTGTTTACCTGCTATATTTCTAGCTTGTTCACACCAGTAGATGGAGGCTCTCCTAGTCTACCCAAC
CAAGCCCAGCTGAGAAGACTGTGCCAAGTCGCTGCCAAAGGAATATGGACTATGACTTACGTGTTTTACA
GAGAAAATCTCAGAAGGCTTGGGTTAACTCAATCTGATGTCTCTAGTTTTATGGACAGCAATATTATTCA
GAAGGACGCAGAGTATGAAAACTGCTATGTGTTCACCCACCTTCATGTTCAGGAGTTTTTTGCAGCTATG
TTCTATATGTTGAAAGGCAGTTGGGAAGCTGGGAACCCTTCCTGCCAGCCTTTTGAAGATTTGAAGTCAT
TACTTCAAAGCACAAGTTATAAAGACCCCCATTTGACACAGATGAAGTGCTTTTTGTTTGGCCTTTTGAA
TGAAGATCGAGTAAAACAACTGGAGAGGACTTTTAACTGTAAAATGTCACTGAAGATAAAATCAAAGTTA
CTTCAGTGTATGGAAGTATTAGGAAACAGTGACTATTCTCCATCACAGCTGGGATTTCTGGAGTTGTTTC
ACTGTCTGTATGAGACTCAAGATAAAGCGTTTATAAGCCAGGCAATGAGATGTTTCCCAAAGGTTGCCAT
TAATATTTGTGAGAAAATACATTTGCTTGTATCTTCTTTCTGCCTTAAGCACTGCCGGTGTTTGCGGACC
ATCAGGCTGTCTGTAACTGTGGTATTTGAGAAGAAGATATTAAAAACAAGCCTCCCAACTAACACTTGGG
ATGGTGATCGCATTACTCACTGTTGGCAAGATCTCTGTTCTGTGCTTCATACAAATGAACACTTGAGAGA
ATTGGACCTGTACCATAGCAACCTTGATAAATCAGCAATGAATATCCTGCATCATGAACTAAGGCACCCA
AACTGTAAACTACAAAAGCTACTGTTGAAATTTATCACTTTCCCTGATGGTTGTCAGGATATCTCTACTT
CTTTGATTCATAACAAGAATCTGATGCATCTTGACCTAAAAGGGAGTGATATAGGGGATAATGGAGTAAA
GTCATTGTGTGAGGCCTTGAAACACCCAGAGTGTAAACTACAGACTCTCAGGCTGGAATCTTGCAACCTA
ACTGTATTTTGTTGTCTAAATATATCTAATGCTCTCATCAGAAGCCAGAGCCTGATATTTCTGAATCTGT
CAACCAATAATCTGTTGGATGATGGAGTGCAGCTTTTGTGTGAGGCCTTAAGACATCCAAAGTGTTATCT
AGAGAGACTGTCCTTAGAAAGCTGTGGTCTCACAGAGGCTGGCTGTGAGTATCTTTCTTTGGCTCTCATC
AGCAATAAAAGACTGACACATTTGTGCTTGGCAGACAATGTCTTGGGTGATGGTGGAGTAAAGCTTATGA
GTGATGCCCTGCAACATGCACAATGTACTCTGAAGAGCCTTGTGCTGAGGCGTTGCCATTTCACTTCACT
TAGCAGTGAATATCTGTCAACTTCTCTTCTACACAACAAGAGCCTGACGCATCTGGATCTAGGATCAAAC
TGGCTACAAGACAATGGAGTGAAGCTTCTGTGTGATGTCTTTCGGCATCCAAGCTGTAATCTTCAGGACT
TGGAATTGATGGGCTGTGTTCTCACTAATGCATGTTGTCTGGATCTGGCTTCTGTTATTTTGAATAACCC
AAACCTGAGGAGCCTGGACCTTGGGAACAACGATTTGCAGGATGATGGAGTGAAAATTCTGTGTGATGCT
TTGAGATATCCAAACTGTAACATTCAGAGGCTCGGGTTGGAATACTGTGGTTTGACATCTCTCTGCTGTC
AAGATCTCTCCTCTGCTCTTATCTGCAACAAAAGACTGATAAAAATGAATCTGACACAGAATACCTTAGG
ATATGAAGGAATTGTGAAGTTATATAAAGTCTTGAAGTCTCCTAAGTGTAAACTACAAGTTCTAGGGTTG
TGCAAAGAGGCATTTGATGAGGAAGCCCAGAAGCTGCTGGAAGCTGTGGGAGTTAGCAATCCACACTTAA
TCATTAAGCCAGATTGTAACTATCATAATGAAGAAGATGTGTCTTGGTGGTGGTGTTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215050 representing NM_176822
Red=Cloning site Green=Tags(s)

MADSSSSSFFPDFGLLLYLEELNKEELNTFKLFLKETMEPEHGLTPWNEVKKARREDLANLMKKYYPGEK
AWSVSLKIFGKMNLKDLCERAKEEINWSAQTIGPDDAKAGETQEDQEAVLGDGTEYRNRIKEKFCITWDK
KSLAGKPEDFHHGIAEKDRKLLEHLFDVDVKTGAQPQIVVLQGAAGVGKTTLVRKAMLDWAEGSLYQQRF
KYVFYLNGREINQLKERSFAQLISKDWPSTEGPIEEIMYQPSSLLFIIDSFDELNFAFEEPEFALCEDWT
QEHPVSFLMSSLLRKVMLPEASLLVTTRLTTSKRLKQLLKNHHYVELLGMSEDAREEYIYQFFEDKRWAM
KVFSSLKSNEMLFSMCQVPLVCWAACTCLKQQMEKGGDVTLTCQTTTALFTCYISSLFTPVDGGSPSLPN
QAQLRRLCQVAAKGIWTMTYVFYRENLRRLGLTQSDVSSFMDSNIIQKDAEYENCYVFTHLHVQEFFAAM
FYMLKGSWEAGNPSCQPFEDLKSLLQSTSYKDPHLTQMKCFLFGLLNEDRVKQLERTFNCKMSLKIKSKL
LQCMEVLGNSDYSPSQLGFLELFHCLYETQDKAFISQAMRCFPKVAINICEKIHLLVSSFCLKHCRCLRT
IRLSVTVVFEKKILKTSLPTNTWDGDRITHCWQDLCSVLHTNEHLRELDLYHSNLDKSAMNILHHELRHP
NCKLQKLLLKFITFPDGCQDISTSLIHNKNLMHLDLKGSDIGDNGVKSLCEALKHPECKLQTLRLESCNL
TVFCCLNISNALIRSQSLIFLNLSTNNLLDDGVQLLCEALRHPKCYLERLSLESCGLTEAGCEYLSLALI
SNKRLTHLCLADNVLGDGGVKLMSDALQHAQCTLKSLVLRRCHFTSLSSEYLSTSLLHNKSLTHLDLGSN
WLQDNGVKLLCDVFRHPSCNLQDLELMGCVLTNACCLDLASVILNNPNLRSLDLGNNDLQDDGVKILCDA
LRYPNCNIQRLGLEYCGLTSLCCQDLSSALICNKRLIKMNLTQNTLGYEGIVKLYKVLKSPKCKLQVLGL
CKEAFDEEAQKLLEAVGVSNPHLIIKPDCNYHNEEDVSWWWCF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_176822
ORF Size 3279 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_176822.4
RefSeq Size 3521 bp
RefSeq ORF 3282 bp
Locus ID 338323
UniProt ID Q86W24
Cytogenetics 11p15.4
MW 124.6 kDa
Gene Summary The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.