DUSP16 (NM_030640) Human Tagged ORF Clone

CAT#: RC214452

DUSP16 (Myc-DDK-tagged)-Human dual specificity phosphatase 16 (DUSP16)

Product Images

Circular map for RC214452

• 

  Western blot validation of overexpression lysate (Cat# LY410762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214452 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DUSP16
• Synonyms: MKP-7; MKP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214452 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATGAGATGATTGGAACTCAAATTGTTACTGAGAGGTTGGTGGCTCTGCTGGAAAGTGGAACGG
  AAAAAGTGCTGCTAATTGATAGCCGGCCATTTGTGGAATACAATACATCCCACATTTTGGAAGCCATTAA
  TATCAACTGCTCCAAGCTTATGAAGCGAAGGTTGCAACAGGACAAAGTGTTAATTACAGAGCTCATCCAG
  CATTCAGCGAAACATAAGGTTGACATTGATTGCAGTCAGAAGGTTGTAGTTTACGATCAAAGCTCCCAAG
  ATGTTGCCTCTCTCTCTTCAGACTGTTTTCTCACTGTACTTCTGGGTAAACTGGAGAAGAGCTTCAACTC
  TGTTCACCTGCTTGCAGGTGGGTTTGCTGAGTTCTCTCGTTGTTTCCCTGGCCTCTGTGAAGGAAAATCC
  ACTCTAGTCCCTACCTGCATTTCTCAGCCTTGCTTACCTGTTGCCAACATTGGGCCAACCCGAATTCTTC
  CCAATCTTTATCTTGGCTGCCAGCGAGATGTCCTCAACAAGGAGCTGATGCAGCAGAATGGGATTGGTTA
  TGTGTTAAATGCCAGCAATACCTGTCCAAAGCCTGACTTTATCCCCGAGTCTCATTTCCTGCGTGTGCCT
  GTGAATGACAGCTTTTGTGAGAAAATTTTGCCGTGGTTGGACAAATCAGTAGATTTCATTGAGAAAGCAA
  AAGCCTCCAATGGATGTGTTCTAGTGCACTGTTTAGCTGGGATCTCCCGCTCCGCCACCATCGCTATCGC
  CTACATCATGAAGAGGATGGACATGTCTTTAGATGAAGCTTACAGATTTGTGAAAGAAAAAAGACCTACT
  ATATCTCCAAACTTCAATTTTCTGGGCCAACTCCTGGACTATGAGAAGAAGATTAAGAACCAGACTGGAG
  CATCAGGGCCAAAGAGCAAACTCAAGCTGCTGCACCTGGAGAAGCCAAATGAACCTGTCCCTGCTGTCTC
  AGAGGGTGGACAGAAAAGCGAGACGCCCCTCAGTCCACCCTGTGCCGACTCTGCTACCTCAGAGGCAGCA
  GGACAAAGGCCCGTGCATCCCGCCAGCGTGCCCAGCGTGCCCAGCGTGCAGCCGTCGCTGTTAGAGGACA
  GCCCGCTGGTACAGGCGCTCAGTGGGCTGCACCTGTCCGCAGACAGGCTGGAAGACAGCAATAAGCTCAA
  GCGTTCCTTCTCTCTGGATATCAAATCAGTTTCATATTCAGCCAGCATGGCAGCATCCTTACATGGCTTC
  TCCTCATCAGAAGATGCTTTGGAATACTACAAACCTTCCACTACTCTGGATGGGACCAACAAGCTATGCC
  AGTTCTCCCCTGTTCAGGAACTATCGGAGCAGACTCCCGAAACCAGTCCTGATAAGGAGGAAGCCAGCAT
  CCCCAAGAAGCTGCAGACTGCCAGGCCTTCAGACAGCCAGAGCAAGCGATTGCATTCGGTCAGAACCAGC
  AGCAGTGGCACCGCCCAGAGGTCCCTTTTATCTCCACTGCTTCGAAGTGGGAGCGTGGAGGACAATTACC
  ACACCAGCTTCCTTTTCGGCCTTTCCACCAGCCAGCAGCACCTCACGAAGTCTGCTGGCCTGGGCCTTAA
  GGGCTGGCACTCGGATATCTTGGCCCCCCAGACCTCTACCCCTTCCCTGACCAGCAGCTGGTATTTTGCC
  ACAGAGTCCTCACACTTCTACTCTGCCTCAGCCATCTACGGAGGCAGTGCCAGTTACTCTGCCTACAGCT
  GCAGCCAGCTGCCCACTTGCGGAGACCAAGTCTATTCTGTGCGCAGGCGGCAGAAGCCAAGTGACAGAGC
  TGACTCGCGGCGGAGCTGGCATGAAGAGAGCCCCTTTGAAAAGCAGTTTAAACGCAGAAGCTGCCAAATG
  GAATTTGGAGAGAGCATCATGTCAGAGAACAGGTCACGGGAAGAGCTGGGGAAAGTGGGCAGTCAGTCTA
  GCTTTTCGGGCAGCATGGAAATCATTGAGGTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHEMIGTQIVTERLVALLESGTEKVLLIDSRPFVEYNTSHILEAININCSKLMKRRLQQDKVLITELIQ
  HSAKHKVDIDCSQKVVVYDQSSQDVASLSSDCFLTVLLGKLEKSFNSVHLLAGGFAEFSRCFPGLCEGKS
  TLVPTCISQPCLPVANIGPTRILPNLYLGCQRDVLNKELMQQNGIGYVLNASNTCPKPDFIPESHFLRVP
  VNDSFCEKILPWLDKSVDFIEKAKASNGCVLVHCLAGISRSATIAIAYIMKRMDMSLDEAYRFVKEKRPT
  ISPNFNFLGQLLDYEKKIKNQTGASGPKSKLKLLHLEKPNEPVPAVSEGGQKSETPLSPPCADSATSEAA
  GQRPVHPASVPSVPSVQPSLLEDSPLVQALSGLHLSADRLEDSNKLKRSFSLDIKSVSYSASMAASLHGF
  SSSEDALEYYKPSTTLDGTNKLCQFSPVQELSEQTPETSPDKEEASIPKKLQTARPSDSQSKRLHSVRTS
  SSGTAQRSLLSPLLRSGSVEDNYHTSFLFGLSTSQQHLTKSAGLGLKGWHSDILAPQTSTPSLTSSWYFA
  TESSHFYSASAIYGGSASYSAYSCSQLPTCGDQVYSVRRRQKPSDRADSRRSWHEESPFEKQFKRRSCQM
  EFGESIMSENRSREELGKVGSQSSFSGSMEIIEVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030640
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030640.3
• RefSeq Size: 6312 bp
• RefSeq ORF: 1998 bp
• Locus ID: 80824
• UniProt ID: Q9BY84
• Cytogenetics: 12p13.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: MAPK signaling pathway
• MW: 73.1 kDa
• Gene Summary: This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]