FOXD4 (NM_207305) Human Tagged ORF Clone

CAT#: RC213792

FOXD4 (Myc-DDK-tagged)-Human forkhead box D4 (FOXD4)

Product Images

Circular map for RC213792

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  Western blot validation of overexpression lysate (Cat# LY404084) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213792 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FOXD4 protein (Cat# TP313792). The protein was produced from HEK293T cells transfected with FOXD4 cDNA clone (Cat# RC213792) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXD4
• Synonyms: FKHL9; FOXD4A; FREAC-5; FREAC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213792 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTGCCAAGAGCTGAGCGCCTTCGCTCCACACCGCAGCGCAGCCTCCGGGACTCCGATGGGGAAG
  ACGGTAAAATCGATGTCCTGGGAGAGGAGGAAGATGAAGACGAGGAGGAGGCGGCGAGCCAGCAGTTCCT
  AGAGCAGTCGCTCCAGCCGGGGCTGCAGGTGGCCCGGTGGGGCGGGGTTGCGCTTCCCCGAGAGCACATC
  GAGGGCGGCGGCGGCCCGAGCGACCCCTCAGAGTTTGGCACCGAGTTCAGGGCACCGCCAAGGTCTGCGG
  CGGCCTCTGAAGATGCCCGGCAGCCGGCAAAGCCCCCCTCCTCGTACATCGCGCTCATCACCATGGCCAT
  CCTGCAAAGCCCGCACAAGCGCCTCACGCTCAGCGGCATCTGCGCCTTCATTAGTGACCGCTTCCCCTAC
  TACCGCCGCAAGTTCCCCGCCTGGCAGAACAGCATCCGCCACAACCTCTCGCTGAACGACTGCTTCGTCA
  AGATCCCCCGCGAGCCGGGCCGCCCAGGCAAGGGCAACTACTGGAGCCTGGACCCCGCCTCCCAGGACAT
  GTTCGACAATGGCAGCTTTCTCCGGCGTAGGAAGCGTTTCCAGCGCCACCAACCGACCCCGGGAGCCCAC
  CTGCCCCACCCCTTCCCTCTACCTGCTGCACACGCCGCCCTGCACAACCCCCGCCCAGGCCCTCTGCTTG
  GGGCCCCTGCCCCGCCGCAGCCAGTCCCGGGGGCCTACCCCAACACCGGCCCCGGGAGACGCCCTTACGC
  TCTGCTGCACCCGCATCCTCCTCGCTACCTACTGCTCTCGGCCCCCGCCTATGCCGGGGCACCGAAGAAA
  GCAGAAGGCGCGGACCTGGCGACCCCGGCACCCTTCCCGTGCTGCAGCCCTCACTTGGTCCTCAGCCTTG
  GGAGGAGGGCAAGGGTCTGGCGTCGCCACCGGGAGGCGGATGCATCTCTTTCAGCATTGAGAGTATCATG
  CAAGGGGTCAGGGGAGCGGGTACAGGGGCTGCGCAGAGTTTGTCCCCGACCGCGTGGAGCTACTGCCCCC
  TGCTCCAGCGACCGTCAAGCCTGTCGGACAATTTTGCAGCAACAGCAGCGGCATCAGGAGGAGGACTGCG
  CCAACGGCTGCGCTCCCACCAAGGGCGCGGTGCTGGGCGGGCACCTGTCGGCCGCGTCGGCGCTGCTGCG
  GTATCAGGCGGTGGCAGAGGGCTCTGGGCTGACATCGCTGGCCGCCCCTTTGGGCGGAGAGGGGACCTCA
  CCAGTTTTTTTAGTATCGCCCACGCCCAGTTCCCTGGCCGAGTCCGCAGGGCCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213792 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLPRAERLRSTPQRSLRDSDGEDGKIDVLGEEEDEDEEEAASQQFLEQSLQPGLQVARWGGVALPREHI
  EGGGGPSDPSEFGTEFRAPPRSAAASEDARQPAKPPSSYIALITMAILQSPHKRLTLSGICAFISDRFPY
  YRRKFPAWQNSIRHNLSLNDCFVKIPREPGRPGKGNYWSLDPASQDMFDNGSFLRRRKRFQRHQPTPGAH
  LPHPFPLPAAHAALHNPRPGPLLGAPAPPQPVPGAYPNTGPGRRPYALLHPHPPRYLLLSAPAYAGAPKK
  AEGADLATPAPFPCCSPHLVLSLGRRARVWRRHREADASLSALRVSCKGSGERVQGLRRVCPRPRGATAP
  CSSDRQACRTILQQQQRHQEEDCANGCAPTKGAVLGGHLSAASALLRYQAVAEGSGLTSLAAPLGGEGTS
  PVFLVSPTPSSLAESAGPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207305
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207305.5
• RefSeq Size: 2204 bp
• RefSeq ORF: 1320 bp
• Locus ID: 2298
• UniProt ID: Q12950
• Cytogenetics: 9p24.3
• Protein Families: Transcription Factors
• MW: 47.3 kDa
• Gene Summary: This gene encodes a member of the forkhead/winged helix-box (FOX) family of transcription factors. FOX transcription factors play critical roles in the regulation of multiple processes including metabolism, cell proliferation and gene expression during ontogenesis. Mutations in this gene are associated with a complex phenotype consisting of dilated cardiomyopathy, obsessive-compulsive disorders, and suicidality. [provided by RefSeq, Mar 2012]