NFH (NEFH) (NM_021076) Human Tagged ORF Clone

CAT#: RC213487

NEFH (Myc-DDK-tagged)-Human neurofilament, heavy polypeptide (NEFH)

Product Images

Circular map for RC213487

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NEFH (Cat# RC213487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFH (Cat# TA801161). Positive lysates LY412101 (100ug) and LC412101 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY412101) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213487 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NEFH protein (Cat# TP313487). The protein was produced from HEK293T cells transfected with NEFH cDNA clone (Cat# RC213487) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NFH
• Synonyms: CMT2CC; NFH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213487 representing NM_021076
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGCTTCGGCGGCGCGGACGCGCTGCTGGGCGCCCCGTTCGCGCCGCTGCATGGCGGCGGCAGCC
  TCCACTACGCGCTAGCCCGAAAGGGTGGCGCAGGCGGGACGCGCTCCGCCGCTGGCTCCTCCAGCGGCTT
  CCACTCGTGGACACGGACGTCCGTGAGCTCCGTGTCCGCCTCGCCCAGCCGCTTCCGTGGCGCAGGCGCC
  GCCTCAAGCACCGACTCGCTGGACACGCTGAGCAACGGGCCGGAGGGCTGCATGGTGGCGGTGGCCACCT
  CACGCAGTGAGAAGGAGCAGCTGCAGGCGCTGAACGACCGCTTCGCCGGGTACATCGACAAGGTGCGGCA
  GCTGGAGGCGCACAACCGCAGCCTGGAGGGCGAGGCTGCGGCGCTGCGGCAGCAGCAGGCGGGCCGCTCC
  GCTATGGGCGAGCTGTACGAGCGCGAGGTCCGCGAGATGCGCGGCGCGGTGCTGCGCCTGGGCGCGGCGC
  GCGGTCAGCTACGCCTGGAGCAGGAGCACCTGCTCGAGGACATCGCGCACGTGCGCCAGCGCCTAGACGA
  CGAGGCCCGGCAGCGAGAGGAGGCCGAGGCGGCGGCCCGCGCGCTGGCGCGCTTCGCGCAGGAGGCCGAG
  GCGGCGCGCGTGGACCTGCAGAAGAAGGCGCAGGCGCTGCAGGAGGAGTGCGGCTACCTGCGGCGCCAYC
  ACCAGGAAGAGGTGGGCGAGCTGCTCGGCCAGATCCAGGGCTCCGGCGCCGCGCAGGCGCAGATGCAGGC
  CGAGACGCGCGACGCCCTGAAGTGCGACGTGACGTCGGCGCTGCGCGAGATTCGCGCGCAGCTTGAAGGC
  CACGCGGTGCAGAGCACGCTGCAGTCCGAGGAGTGGTTCCGAGTGAGGCTGGACCGACTGTCGGAGGCAG
  CCAAGGTGAACACAGACGCTATGCGCTCAGCGCAGGAGGAGATAACTGAGTACCGGCGTCAGCTGCAGGC
  CAGGACCACAGAGCTGGAGGCACTGAAAAGCACCAAGGACTCACTGGAGAGGCAGCGCTCTGAGCTGGAG
  GACCGTCATCAGGCCGACATTGCCTCCTACCAGGAAGCCATTCAGCAGCTGGACGCTGAGCTGAGGAACA
  CCAAGTGGGAGATGGCCGCCCAGCTGCGAGAATACCAGGACCTGCTCAATGTCAAGATGGCTCTGGATAT
  AGAGATAGCCGCTTACAGAAAACTCCTGGAAGGTGAAGAGTGTCGGATTGGCTTTGGCCCAATTCCTTTC
  TCGCTTCCAGAAGGACTCCCCAAAATTCCCTCTGTGTCCACTCACATAAAGGTGAAAAGCGAAGAGAAGA
  TCAAAGTGGTGGAGAAGTCTGAGAAAGAAACTGTGATTGTGGAGGAACAGACAGAGGAGACCCAAGTGAC
  TGAAGAAGTGACTGAAGAAGAGGAGAAAGAGGCCAAAGAGGAGGAGGGCAAGGAGGAAGAAGGGGGTGAA
  GAAGAGGAGGCAGAAGGGGGAGAAGAAGAAACAAAGTCTCCCCCAGCAGAAGAGGCTGCATCCCCAGAGA
  AGGAAGCCAAGTCACCAGTAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCCCCAGAGAAGGAGGA
  AGCAAAATCCCCAGCCGAAGTCAAGTCCCCTGAGAAGGCCAAGTCTCCAGCAAAGGAAGAGGCAAAGTCA
  CCGCCTGAGGCCAAGTCCCCAGAGAAGGAGGAAGCAAAATCTCCAGCTGAGGTCAAGTCCCCCGAGAAGG
  CCAAGTCCCCAGCAAAGGAAGAGGCAAAGTCACCGGCTGAGGCCAAGTCTCCAGAGAAGGCCAAGTCCCC
  AGTGAAGGAAGAAGCAAAGTCACTGGCTGAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAATCTCCAGCT
  GAGGTCAAGTCCCCGGAAAAGGCCAAGTCTCCAACGAAGGAGGAAGCAAAGTCCCCTGAGAAGGCCAAGT
  CCCCAGAGAAGGAAGAGGCCAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCC
  TGAGAAGGCCAAGTCCCCAGTGAAGGCAGAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAA
  GAAGCAAAGTCCCCTGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCAAAGTCCCCTGAGAAGGCCAAGT
  CCCCAGTGAAGGAAGAAGCAAAGACCCCCGAGAAGGCCAAGTCCCCAGTGAAGGAAGAAGCTAAGTCCCC
  AGAGAAGGCCAAGTCCCCAGAGAAGGCCAAGACTCTTGATGTGAAGTCTCCAGAAGCCAAGACTCCAGCG
  AAGGAGGAAGCAAGGTCCCCTGCAGACAAATTCCCTGAAAAGGCCAAAAGCCCTGTCAAGGAGGAGGTCA
  AGTCCCCAGAGAAGGCGAAATCTCCCCTGAAGGAGGATGCCAAGGCCCCTGAGAAGGAGATCCCAAAAAA
  GGAAGAGGTGAAGTCCCCAGTGAAGGAGGAGGAGAAGCCCCAGGAGGTGAAAGTCAAAGAGCCCCCAAAG
  AAGGCAGAGGAAGAGAAAGCCCCTGCCACACCAAAAACAGAGGAGAAGAAGGACAGCAAGAAAGAGGAGG
  CACCCAAGAAGGAGGCTCCAAAGCCCAAGGTGGAGGAGAAGAAGGAACCTGCTGTCGAAAAGCCCAAAGA
  ATCCAAAGTTGAAGCCAAGAAGGAAGAGGCTGAAGATAAGAAAAAAGTCCCCACCCCAGAGAAGGAGGCT
  CCTGCCAAGGTGGAGGTGAAGGAAGACGCTAAACCCAAAGAAAAGACAGAGGTAGCCAAGAAGGAACCAG
  ATGATGCCAAGGCCAAGGAACCCAGCAAACCAGCAGAGAAGAAGGAGGCAGCACCGGAGAAAAAAGACAC
  CAAGGAGGAGAAGGCCAAGAAGCCTGAGGAGAAACCCAAGACAGAGGCCAAAGCCAAGGAAGATGACAAG
  ACCCTCTCAAAAGAGCCTAGCAAGCCTAAGGCAGAAAAGGCTGAAAAATCCTCCAGCACAGACCAAAAAG
  ACAGCAAGCCTCCAGAGAAGGCCACAGAAGACAAGGCCGCCAAGGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213487 representing NM_021076
  Red=Cloning site Green=Tags(s)
  
  MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGA
  ASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRS
  AMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAE
  AARVDLQKKAQALQEECGYLRRXHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEG
  HAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELE
  DRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPF
  SLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGE
  EEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKS
  PPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSLAEAKSPVKEEAKSPA
  EVKSPEKAKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKE
  EAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPA
  KEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPK
  KAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEA
  PAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDK
  TLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021076
• ORF Size: 3060 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021076.4
• RefSeq Size: 3695 bp
• RefSeq ORF: 3063 bp
• Locus ID: 4744
• UniProt ID: P12036
• Cytogenetics: 22q12.2
• Domains: filament
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• MW: 111.7 kDa
• Gene Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]