RSK4 (RPS6KA6) (NM_014496) Human Tagged ORF Clone

CAT#: RC211545

RPS6KA6 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)

Product Images

Circular map for RC211545

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  Western blot validation of overexpression lysate (Cat# LY402340) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211545 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RPS6KA6 protein (Cat# TP311545). The protein was produced from HEK293T cells transfected with RPS6KA6 cDNA clone (Cat# RC211545) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RSK4
• Synonyms: p90RSK6; PP90RSK4; RSK-4; RSK4; S6K-alpha-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211545 representing NM_014496
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGACCGAGAAATGGAAGTGTTCAGCGGCGGCGGCGCGA
  GCAGCGGCGAGGTAAATGGTCTTAAAATGGTTGATGAGCCAATGGAAGAGGGAGAAGCAGATTCTTGTCA
  TGATGAAGGAGTTGTTAAAGAAATCCCTATTACTCATCATGTTAAGGAAGGCTATGAGAAAGCAGATCCT
  GCACAGTTTGAGTTGCTCAAGGTTCTTGGTCAGGGGTCATTTGGAAAGGTTTTTCTTGTTAGAAAGAAGA
  CCGGTCCTGATGCTGGGCAGCTCTATGCAATGAAGGTGTTAAAAAAAGCCTCTTTAAAAGTTCGAGACAG
  AGTTCGGACAAAGATGGAGAGGGATATACTGGTGGAAGTAAATCATCCATTTATTGTCAAATTGCACTAT
  GCCTTTCAGACTGAAGGGAAACTGTACTTAATACTGGATTTTCTCAGGGGAGGAGATGTTTTCACAAGAT
  TATCCAAAGAGGTTCTGTTTACAGAGGAAGATGTGAAATTCTACCTCGCAGAACTGGCCCTTGCTTTGGA
  TCATCTGCACCAATTAGGAATTGTTTATAGAGACCTGAAGCCAGAAAACATTTTGCTTGATGAAATAGGA
  CATATCAAATTAACAGATTTTGGACTCAGCAAGGAGTCAGTAGATCAAGAAAAGAAGGCTTACTCATTTT
  GTGGTACAGTAGAGTATATGGCTCCTGAAGTAGTAAATAGGAGAGGCCATTCCCAGAGTGCTGATTGGTG
  GTCATATGGTGTTCTTATGTTTGAAATGCTTACTGGTACTCTGCCATTTCAAGGTAAAGACAGAAATGAG
  ACCATGAATATGATATTAAAAGCAAAACTTGGAATGCCTCAATTTCTTAGTGCTGAAGCACAAAGTCTTC
  TAAGGATGTTATTCAAAAGGAATCCAGCAAATAGATTGGGATCAGAAGGAGTTGAAGAAATCAAAAGACA
  TCTGTTTTTTGCAAATATTGACTGGGATAAATTATATAAAAGAGAAGTTCAACCTCCTTTCAAACCTGCT
  TCTGGAAAACCAGATGATACTTTTTGTTTTGATCCTGAATTTACTGCAAAAACACCTAAAGATTCTCCCG
  GTTTGCCAGCCAGTGCAAATGCTCATCAGCTCTTCAAAGGATTCAGCTTTGTTGCAACTTCTATTGCAGA
  AGAATATAAAATCACTCCTATCACAAGTGCAAATGTATTACCAATTGTTCAGATAAATGGAAATGCTGCA
  CAATTTGGTGAAGTATATGAATTGAAGGAGGATATTGGTGTTGGCTCCTACTCTGTTTGCAAGCGATGCA
  TACATGCAACTACCAACATGGAATTTGCAGTGAAGATCATTGACAAAAGTAAGCGAGACCCTTCAGAAGA
  GATTGAAATATTGATGCGCTATGGACAACATCCCAACATTATTACTTTGAAGGATGTCTTTGATGATGGT
  AGATATGTTTACCTTGTTACGGATTTAATGAAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAAT
  GTTTCTCGGAACGGGAGGCTAGTGATATACTATATGTAATAAGTAAGACAGTTGACTATCTTCATTGTCA
  AGGAGTTGTTCATCGTGATCTTAAACCTAGTAATATTTTATACATGGATGAATCAGCCAGTGCAGATTCA
  ATCAGGATATGTGATTTTGGGTTTGCAAAACAACTTCGAGGAGAAAATGGACTTCTCTTAACTCCATGCT
  ACACTGCAAACTTTGTTGCACCTGAGGTTCTTATGCAACAGGGATATGATGCTGCTTGTGATATCTGGAG
  TTTAGGAGTCCTTTTTTACACAATGTTGGCTGGCTACACTCCATTTGCTAATGGCCCCAATGATACTCCT
  GAAGAGATACTGCTGCGTATAGGCAATGGAAAATTCTCTTTGAGTGGTGGAAACTGGGACAATATTTCAG
  ACGGAGCAAAGGATTTGCTTTCCCATATGCTTCATATGGACCCACATCAGCGGTATACTGCTGAACAAAT
  ATTAAAGCACTCATGGATAACTCACAGAGACCAGTTGCCAAATGATCAGCCAAAGAGAAATGATGTGTCA
  CATGTTGTTAAGGGAGCAATGGTTGCAACATACTCTGCCCTGACTCACAAGACCTTTCAACCAGTCCTAG
  AGCCTGTAGCTGCTTCAAGCTTAGCCCAGCGACGGAGCATGAAAAAGCGAACATCAACTGGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211545 representing NM_014496
  Red=Cloning site Green=Tags(s)
  
  MLPFAPQDEPWDREMEVFSGGGASSGEVNGLKMVDEPMEEGEADSCHDEGVVKEIPITHHVKEGYEKADP
  AQFELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHY
  AFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIG
  HIKLTDFGLSKESVDQEKKAYSFCGTVEYMAPEVVNRRGHSQSADWWSYGVLMFEMLTGTLPFQGKDRNE
  TMNMILKAKLGMPQFLSAEAQSLLRMLFKRNPANRLGSEGVEEIKRHLFFANIDWDKLYKREVQPPFKPA
  SGKPDDTFCFDPEFTAKTPKDSPGLPASANAHQLFKGFSFVATSIAEEYKITPITSANVLPIVQINGNAA
  QFGEVYELKEDIGVGSYSVCKRCIHATTNMEFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVFDDG
  RYVYLVTDLMKGGELLDRILKQKCFSEREASDILYVISKTVDYLHCQGVVHRDLKPSNILYMDESASADS
  IRICDFGFAKQLRGENGLLLTPCYTANFVAPEVLMQQGYDAACDIWSLGVLFYTMLAGYTPFANGPNDTP
  EEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVS
  HVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014496
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014496.5
• RefSeq Size: 2640 bp
• RefSeq ORF: 2238 bp
• Locus ID: 27330
• UniProt ID: Q9UK32
• Cytogenetics: Xq21.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 83.7 kDa
• Gene Summary: This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]