eNOS (NOS3) (NM_000603) Human Tagged ORF Clone

CAT#: RC209228

NOS3 (Myc-DDK-tagged)-Human nitric oxide synthase 3 (endothelial cell) (NOS3), transcript variant 1

Product Images

Circular map for RC209228

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NOS3 (Cat# RC209228, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NOS3(Cat# TA590785). Positive lysates LY424613 (100ug) and LC424613 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY424613) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209228 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NOS3 protein (Cat# TP309228). The protein was produced from HEK293T cells transfected with NOS3 cDNA clone (Cat# RC209228) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: eNOS
• Synonyms: ECNOS; eNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209228 representing NM_000603.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCAACTTGAAGAGCGTGGCCCAGGAGCCTGGGCCACCCTGCGGCCTGGGGCTGGGGCTGGGCCTT
  GGGCTGTGCGGCAAGCAGGGCCCAGCCACCCCGGCCCCTGAGCCCAGCCGGGCCCCAGCATCCCTACTC
  CCACCAGCGCCAGAACACAGCCCCCCGAGCTCCCCGCTAACCCAGCCCCCAGAGGGGCCCAAGTTCCCT
  CGTGTGAAGAACTGGGAGGTGGGGAGCATCACCTATGACACCCTCAGCGCCCAGGCGCAGCAGGATGGG
  CCCTGCACCCCAAGACGCTGCCTGGGCTCCCTGGTATTTCCACGGAAACTACAGGGCCGGCCCTCCCCC
  GGCCCCCCGGCCCCTGAGCAGCTGCTGAGTCAGGCCCGGGACTTCATCAACCAGTACTACAGCTCCATT
  AAGAGGAGCGGCTCCCAGGCCCACGAACAGCGGCTTCAAGAGGTGGAAGCCGAGGTGGCAGCCACAGGC
  ACCTACCAGCTTAGGGAGAGCGAGCTGGTGTTCGGGGCTAAGCAGGCCTGGCGCAACGCTCCCCGCTGC
  GTGGGCCGGATCCAGTGGGGGAAGCTGCAGGTGTTCGATGCCCGGGACTGCAGGTCTGCACAGGAAATG
  TTCACCTACATCTGCAACCACATCAAGTATGCCACCAACCGGGGCAACCTTCGCTCGGCCATCACAGTG
  TTCCCGCAGCGCTGCCCTGGCCGAGGAGACTTCCGAATCTGGAACAGCCAGCTGGTGCGCTACGCGGGC
  TACCGGCAGCAGGACGGCTCTGTGCGGGGGGACCCAGCCAACGTGGAGATCACCGAGCTCTGCATTCAG
  CACGGCTGGACCCCAGGAAACGGTCGCTTCGACGTGCTGCCCCTGCTGCTGCAGGCCCCAGATGAGCCC
  CCAGAACTCTTCCTTCTGCCCCCCGAGCTGGTCCTTGAGGTGCCCCTGGAGCACCCCACGCTGGAGTGG
  TTTGCAGCCCTGGGCCTGCGCTGGTACGCCCTCCCGGCAGTGTCCAACATGCTGCTGGAAATTGGGGGC
  CTGGAGTTCCCCGCAGCCCCCTTCAGTGGCTGGTACATGAGCACTGAGATCGGCACGAGGAACCTGTGT
  GACCCTCACCGCTACAACATCCTGGAGGATGTGGCTGTCTGCATGGACCTGGATACCCGGACCACCTCG
  TCCCTGTGGAAAGACAAGGCAGCAGTGGAAATCAACGTGGCCGTGCTGCACAGTTACCAGCTAGCCAAA
  GTCACCATCGTGGACCACCACGCCGCCACGGCCTCTTTCATGAAGCACCTGGAGAATGAGCAGAAGGCC
  AGGGGGGGCTGCCCTGCAGACTGGGCCTGGATCGTGCCCCCCATCTCGGGCAGCCTCACTCCTGTTTTC
  CATCAGGAGATGGTCAACTATTTCCTGTCCCCGGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGGAGT
  GCCGCCAAGGGCACCGGCATCACCAGGAAGAAGACCTTTAAAGAAGTGGCCAACGCCGTGAAGATCTCC
  GCCTCGCTCATGGGCACGGTGATGGCGAAGCGAGTGAAGGCGACAATCCTGTATGGCTCCGAGACCGGC
  CGGGCCCAGAGCTACGCACAGCAGCTGGGGAGACTCTTCCGGAAGGCTTTTGATCCCCGGGTCCTGTGT
  ATGGATGAGTATGACGTGGTGTCCCTCGAACACGAGACGCTGGTGCTGGTGGTAACCAGCACATTTGGG
  AATGGGGATCCCCCGGAGAATGGAGAGAGCTTTGCAGCTGCCCTGATGGAGATGTCCGGCCCCTACAAC
  AGCTCCCCTCGGCCGGAACAGCACAAGAGTTATAAGATCCGCTTCAACAGCATCTCCTGCTCAGACCCA
  CTGGTGTCCTCTTGGCGGCGGAAGAGGAAGGAGTCCAGTAACACAGACAGTGCAGGGGCCCTGGGCACC
  CTCAGGTTCTGTGTGTTCGGGCTCGGCTCCCGGGCATACCCCCACTTCTGCGCCTTTGCTCGTGCGGTG
  GACACACGGCTGGAGGAACTGGGCGGGGAGCGGCTGCTGCAGCTGGGCCAGGGCGACGAGCTGTGCGGC
  CAGGAGGAGGCCTTCCGAGGCTGGGCCCAGGCTGCCTTCCAGGCCGCCTGTGAGACCTTCTGTGTGGGA
  GAGGATGCCAAGGCCGCCGCCCGAGACATCTTCAGCCCCAAACGGAGCTGGAAGCGCCAGAGGTACCGG
  CTGAGCGCCCAGGCCGAGGGCCTGCAGTTGCTGCCAGGTCTGATCCACGTGCACAGGCGGAAGATGTTC
  CAGGCTACAATCCGCTCAGTGGAAAACCTGCAAAGCAGCAAGTCCACGAGGGCCACCATCCTGGTGCGC
  CTGGACACCGGAGGCCAGGAGGGGCTGCAGTACCAGCCGGGGGACCACATAGGTGTCTGCCCGCCCAAC
  CGGCCCGGCCTTGTGGAGGCGCTGCTGAGCCGCGTGGAGGACCCGCCGGCGCCCACTGAGCCCGTGGCA
  GTAGAGCAGCTGGAGAAGGGCAGCCCTGGTGGCCCTCCCCCCGGCTGGGTGCGGGACCCCCGGCTGCCC
  CCGTGCACGCTGCGCCAGGCTCTCACCTTCTTCCTGGACATCACCTCCCCACCCAGCCCTCAGCTCTTG
  CGGCTGCTCAGCACCTTGGCAGAAGAGCCCAGGGAACAGCAGGAGCTGGAGGCCCTCAGCCAGGATCCC
  CGACGCTACGAGGAGTGGAAGTGGTTCCGCTGCCCCACGCTGCTGGAGGTGCTGGAGCAGTTCCCGTCG
  GTGGCGCTGCCTGCCCCACTGCTCCTCACCCAGCTGCCTCTGCTCCAGCCCCGGTACTACTCAGTCAGC
  TCGGCACCCAGCACCCACCCAGGAGAGATCCACCTCACTGTAGCTGTGCTGGCATACAGGACTCAGGAT
  GGGCTGGGCCCCCTGCACTATGGAGTCTGCTCCACGTGGCTAAGCCAGCTCAAGCCCGGAGACCCTGTG
  CCCTGCTTCATCCGGGGGGCTCCCTCCTTCCGGCTGCCACCCGATCCCAGCTTGCCCTGCATCCTGGTG
  GGTCCAGGCACTGGCATTGCCCCCTTCCGGGGATTCTGGCAGGAGCGGCTGCATGACATTGAGAGCAAA
  GGGCTGCAGCCCACTCCCATGACTTTGGTGTTCGGCTGCCGATGCTCCCAACTTGACCATCTCTACCGC
  GACGAGGTGCAGAACGCCCAGCAGCGCGGGGTGTTTGGCCGAGTCCTCACCGCCTTCTCCCGGGAACCT
  GACAACCCCAAGACCTACGTGCAGGACATCCTGAGGACGGAGCTGGCTGCGGAGGTGCACCGCGTGCTG
  TGCCTCGAGCGGGGCCACATGTTTGTCTGCGGCGATGTTACCATGGCAACCAACGTCCTGCAGACCGTG
  CAGCGCATCCTGGCGACGGAGGGCGACATGGAGCTGGACGAGGCCGGCGACGTCATCGGCGTGCTGCGG
  GATCAGCAACGCTACCACGAAGACATTTTCGGGCTCACGCTGCGCACCCAGGAGGTGACAAGCCGCATA
  CGCACCCAGAGCTTTTCCTTGCAGGAGCGTCAGTTGCGGGGCGCAGTGCCCTGGGCGTTCGACCCTCCC
  GGCTCAGACACCAACAGCCCC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC209228
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFP
  RVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSI
  KRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEM
  FTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQ
  HGWTPGNGRFDVLPLLLQAPDEPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGG
  LEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAK
  VTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGS
  AAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLC
  MDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDP
  LVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCG
  QEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMF
  QATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVA
  VEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDP
  RRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQD
  GLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESK
  GLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVL
  CLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRI
  RTQSFSLQERQLRGAVPWAFDPPGSDTNSP
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC209228 also available, TP309228
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000603
• ORF Size: 3609 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4345 bp
• RefSeq ORF: 3612 bp
• Locus ID: 4846
• UniProt ID: P29474
• Cytogenetics: 7q36.1
• Domains: flavodoxin, NAD_binding_1, FAD_binding_1, NO_synthase
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway
• MW: 133.3 kDa
• Gene Summary: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]