NPAS2 (NM_002518) Human Tagged ORF Clone

CAT#: RC208549

NPAS2 (Myc-DDK-tagged)-Human neuronal PAS domain protein 2 (NPAS2)

Product Images

Circular map for RC208549

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NPAS2
• Synonyms: bHLHe9; MOP4; PASD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208549 representing NM_002518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGATGAGAAAGACAGAGCCAAGAGAGCTTCTCGAAACAAGTCTGAGAAGAAGCGTCGGGACC
  AGTTCAATGTTCTCATCAAAGAGCTCAGTTCCATGCTCCCTGGCAACACGCGGAAAATGGACAAAACCAC
  CGTGTTGGAAAAGGTCATCGGATTTTTGCAGAAACACAATGAAGTCTCAGCGCAAACGGAAATCTGTGAC
  ATTCAGCAAGACTGGAAGCCTTCATTCCTCAGTAATGAAGAATTCACCCAGCTGATGTTGGAGGCATTAG
  ATGGCTTCATTATCGCAGTGACAACAGACGGCAGCATCATCTATGTCTCTGACAGTATCACGCCTCTCCT
  TGGGCATTTACCGTCGGATGTCATGGATCAGAATTTGTTAAATTTCCTCCCAGAACAAGAACATTCAGAA
  GTTTATAAAATCCTTTCTTCCCATATGCTTGTGACGGATTCCCCCTCCCCAGAATACTTAAAATCTGACA
  GCGATTTAGAGTTTTATTGCCATCTTCTCAGAGGCAGCTTGAACCCAAAGGAATTTCCAACTTATGAATA
  CATAAAATTTGTAGGAAATTTTCGCTCTTACAACAATGTGCCTAGCCCCTCCTGTAATGGTTTTGACAAC
  ACCCTTTCAAGACCTTGCCGGGTGCCACTAGGAAAGGAGGTTTGCTTCATTGCCACCGTTCGTCTGGCAA
  CACCACAATTCTTAAAGGAAATGTGCATAGTTGACGAACCTTTAGAGGAATTCACTTCAAGGCATAGCTT
  GGAATGGAAATTTTTATTTCTGGATCACAGAGCACCTCCAATCATAGGATACCTGCCTTTTGAAGTGCTG
  GGAACCTCAGGCTATGACTACTACCACATTGATGACCTGGAGCTCCTGGCCAGGTGTCACCAGCACCTGA
  TGCAGTTTGGCAAAGGGAAGTCGTGTTGCTACCGGTTTCTGACCAAAGGTCAGCAGTGGATCTGGCTGCA
  GACTCACTACTACATCACCTACCATCAGTGGAACTCCAAGCCCGAGTTCATCGTGTGCACACACTCGGTG
  GTCAGTTACGCAGATGTCCGGGTGGAAAGGAGGCAGGAGCTGGCTCTGGAAGACCCGCCATCCGAGGCCC
  TCCACTCCTCAGCACTAAAGGACAAGGGCTCAAGCCTGGAACCTCGGCAGCACTTTAACACACTCGACGT
  GGGTGCCTCGGGCCTTAATACCAGTCATTCGCCATCGGCGTCCTCAAGAAGTTCCCACAAATCCTCGCAC
  ACAGCCATGTCAGAACCCACCTCCACTCCCACCAAGCTGATGGCAGAGGCCAGCACCCCGGCTTTGCCAA
  GATCAGCCACCCTGCCCCAAGAGTTACCTGTCCCCGGGCTCAGCCAGGCAGCCACCATGCCGGCCCCTCT
  GCCTTCCCCATCGTCCTGCGACCTCACACAGCAGCTCCTGCCTCAGACCGTTCTGCAGAGCACGCCCGCT
  CCCATGGCACAGTTTTCGGCACAGTTCAGCATGTTCCAGACCATCAAAGACCAGCTAGAGCAGCGGACGC
  GGATCCTGCAGGCCAATATCCGGTGGCAACAGGAAGAGCTCCACAAGATCCAGGAGCAGCTCTGCCTGGT
  CCAGGACTCCAACGTCCAGATGTTCCTGCAGCAGCCAGCTGTATCCCTGAGCTTCAGCAGCACCCAGCGA
  CCTGAGGCTCAGCAGCAGCTACAGCAAAGGTCAGCTGCAGTGACTCAGCCCCAGCTCGGGGCGGGCCCCC
  AACTTCCAGGGCAGATCTCCTCTGCCCAGGTCACAAGCCAGCACCTGCTCAGAGAATCAAGTGTGATATC
  AACCCAGGGTCCAAAGCCAATGAGAAGCTCACAGCTAATGCAGAGCAGCGGCCGCTCTGGAAGCAGCCTA
  GTGTCCCCGTTCAGCAGCGCCACAGCTGCGCTCCCGCCAAGTCTGAATCTGACCACACCTGCTTCCACCT
  CCCAGGATGCCAGCCAGTGCCAGCCCAGCCCAGACTTCAGCCATGATCGGCAGCTCAGGCTGTTGCTGAG
  CCAGCCCATCCAGCCCATGATGCCCGGGTCCTGTGACGCAAGGCAGCCCTCGGAAGTCAGCAGGACGGGA
  CGGCAAGTCAAGTACGCCCAGAGCCAGACCGTGTTTCAAAATCCAGACGCACACCCCGCCAACAGCAGCA
  GCGCCCCGATGCCCGTCCTGCTGATGGGGCAGGCGGTGCTCCACCCCAGCTTCCCTGCCTCCCAACCATC
  GCCCCTGCAGCCTGCACAGGCCCGGCAGCAGCCACCGCAGCACTACCTGCAGGTACAGGCACCAACCTCT
  TTGCACAGTGAGCAGCAGGACTCGCTACTTCTCTCCACCTACTCACAACAGCCAGGGACCCTGGGCTACC
  CCCAACCACCCCCAGCACAGCCCCAGCCCCTACGTCCTCCCCGAAGGGTCAGCAGTCTGTCTGAGTCGTC
  AGGCCTCCAGCAGCCGCCCCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208549 representing NM_002518
  Red=Cloning site Green=Tags(s)
  
  MDEDEKDRAKRASRNKSEKKRRDQFNVLIKELSSMLPGNTRKMDKTTVLEKVIGFLQKHNEVSAQTEICD
  IQQDWKPSFLSNEEFTQLMLEALDGFIIAVTTDGSIIYVSDSITPLLGHLPSDVMDQNLLNFLPEQEHSE
  VYKILSSHMLVTDSPSPEYLKSDSDLEFYCHLLRGSLNPKEFPTYEYIKFVGNFRSYNNVPSPSCNGFDN
  TLSRPCRVPLGKEVCFIATVRLATPQFLKEMCIVDEPLEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVL
  GTSGYDYYHIDDLELLARCHQHLMQFGKGKSCCYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSV
  VSYADVRVERRQELALEDPPSEALHSSALKDKGSSLEPRQHFNTLDVGASGLNTSHSPSASSRSSHKSSH
  TAMSEPTSTPTKLMAEASTPALPRSATLPQELPVPGLSQAATMPAPLPSPSSCDLTQQLLPQTVLQSTPA
  PMAQFSAQFSMFQTIKDQLEQRTRILQANIRWQQEELHKIQEQLCLVQDSNVQMFLQQPAVSLSFSSTQR
  PEAQQQLQQRSAAVTQPQLGAGPQLPGQISSAQVTSQHLLRESSVISTQGPKPMRSSQLMQSSGRSGSSL
  VSPFSSATAALPPSLNLTTPASTSQDASQCQPSPDFSHDRQLRLLLSQPIQPMMPGSCDARQPSEVSRTG
  RQVKYAQSQTVFQNPDAHPANSSSAPMPVLLMGQAVLHPSFPASQPSPLQPAQARQQPPQHYLQVQAPTS
  LHSEQQDSLLLSTYSQQPGTLGYPQPPPAQPQPLRPPRRVSSLSESSGLQQPPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002518
• ORF Size: 2472 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002518.4
• RefSeq Size: 4004 bp
• RefSeq ORF: 2475 bp
• Locus ID: 4862
• UniProt ID: Q99743
• Cytogenetics: 2q11.2
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 91.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq, Jul 2008]