FOXP4 (NM_138457) Human Tagged ORF Clone

CAT#: RC208077

FOXP4 (Myc-DDK-tagged)-Human forkhead box P4 (FOXP4), transcript variant 2

Product Images

Circular map for RC208077

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  Western blot validation of overexpression lysate (Cat# LY408607) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC208077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FOXP4 protein (Cat# TP308077). The protein was produced from HEK293T cells transfected with FOXP4 cDNA clone (Cat# RC208077) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXP4
• Synonyms: hFKHLA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208077 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTGGAATCTGCCTCGGAGACAATCAGGTCGGCTCCATCTGGTCAGAATGGCGTGGGCAGCCTCT
  CTGGGCAAGCCGATGGCAGCAGCGGCGGGGCCACAGGGACAACTGCAAGTGGCACGGGCAGGGAAGTGAC
  CACGGGTGCAGACAGCAATGGTGAGATGAGTCCCGCAGAGCTGCTGCACTTCCAGCAGCAACAGGCTCTC
  CAAGTGGCCCGGCAGTTCCTGCTGCAGCAGGCCTCAGGCCTGAGCTCCCCAGGGAACAATGACAGCAAAC
  AGTCTGCCTCTGCTGTGCAGGTGCCTGTGTCGGTGGCCATGATGTCGCCGCAGATGCTTACCCCGCAACA
  GATGCAGCAGATCCTGTCGCCCCCGCAGCTGCAGGCCTTGCTCCAGCAGCAGCAAGCCCTCATGCTCCAG
  CAGCTACAGGAGTACTACAAGAAGCAGCAGGAGCAGCTCCACCTGCAGCTCCTCACCCAGCAGCAGGCTG
  GGAAACCGCAGCCCAAAGAGGCACTGGGGAACAAGCAGCTGGCCTTCCAGCAGCAGCTCCTGCAAATGCA
  ACAGTTGCAGCAGCAGCACCTGCTCAACCTGCAGAGGCAGGGGCTGGTCAGCCTGCAGCCCAACCAAGCC
  TCGGGGCCCCTCCAGACCCTTCCGCAAGCTGTTTGCCCAACAGACCTGCCCCAGCTGTGGAAGGGCGAGG
  GTGCCCCCGGGCAGCCTGCCGAGGACAGCGTCAAGCAGGAGGGGCTGGACCTCACTGGCACGGCCGCCAC
  CGCTACCTCGTTTGCCGCTCCCCCCAAGGTCTCACCCCCCCTCTCCCACCATACCCTGCCCAACGGACAG
  CCTACTGTGCTCACATCTCGGAGAGACAGCTCTTCCCACGAGGAGACCCCCGGCTCCCACCCCCTGTACG
  GACACGGAGAGTGCAAGTGGCCAGGCTGTGAGACCCTGTGTGAAGACCTGGGCCAGTTTATCAAACACCT
  CAACACAGAGCACGCCCTGGATGACCGGAGTACAGCCCAGTGCCGGGTACAGATGCAGGTGGTGCAGCAG
  CTGGAGATCCAGCTCGCCAAGGAGAGCGAGCGGCTGCAGGCCATGATGGCCCACCTGCACATGCGGCCCT
  CGGAGCCCAAGCCCTTCAGCCAGCCAGTGACCGTCTCTGCAGCAGACTCATTCCCAGATGGTCTCGTGCA
  CCCCCCGACCTCGGCCGCAGCCCCTGTCACCCCTCTACGGCCCCCTGGCCTGGGCTCTGCCTCCCTGCAT
  GGTGGGGGCCCAGCCCGTCGGAGAAGCAGTGACAAGTTCTGCTCCCCCATCTCCTCAGAGCTGGCCCAGA
  ATCATGAGTTCTACAAGAACGCCGACGTCCGGCCCCCCTTCACCTACGCCTCCCTCATCCGCCAGGCCAT
  CCTGGAAACCCCTGACAGGCAGCTGACCCTGAATGAGATCTATAACTGGTTCACCAGGATGTTCGCCTAT
  TTCCGCAGAAACACTGCCACCTGGAAGAACGCCGTGCGCCACAACCTCAGCCTGCACAAGTGCTTCGTCC
  GCGTGGAGAACGTCAAGGGTGCCGTGTGGACTGTGGACGAGCGGGAGTATCAGAAGCGGAGACCGCCAAA
  GATGACAGGGAGCCCCACCCTGGTGAAGAACATGATCTCTGGCCTCAGCTATGGAGCACTTAATGCCAGC
  TACCAGGCCGCCCTGGCCGAGAGCAGCTTCCCCCTCCTCAACAGCCCTGGCATGCTGAACCCTGGCTCCG
  CCAGCAGCCTGCTGCCCCTCAGCCACGATGACGTGGGTGCCCCCGTGGAGCCGCTGCCCAGCAACGGCAG
  CAGCAGCCCTCCTCGCCTCTCCCCGCCCCAGTACAGCCACCAGGTGCAGGTGAAGGAGGAGCCAGCAGAG
  GCAGAGGAAGACAGGCAGCCCGGGCCTCCCCTGGGCGCCCCTAACCCCAGCGCCTCGGGGCCTCCGGAAG
  ACAGGGACCTGGAGGAGGAGCTGCCGGGAGAAGAACTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208077 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQAL
  QVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQ
  QLQEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQA
  SGPLQTLPQAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQ
  PTVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQ
  LEIQLAKESERLQAMMAHLHMRPSEPKPFSQPVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLH
  GGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAY
  FRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNAS
  YQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAE
  AEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138457
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138457.3
• RefSeq Size: 5926 bp
• RefSeq ORF: 2004 bp
• Locus ID: 116113
• UniProt ID: Q8IVH2
• Cytogenetics: 6p21.1
• Protein Families: Transcription Factors
• MW: 72.2 kDa
• Gene Summary: This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. This gene may play a role in the development of tumors of the kidney and larynx. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. [provided by RefSeq, Jul 2008]