POLR3A (NM_007055) Human Tagged ORF Clone

CAT#: RC207553

POLR3A (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide A, 155kDa (POLR3A)

Product Images

Circular map for RC207553

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR3A
• Synonyms: ADDH; C160; HLD7; hRPC155; RPC1; RPC155; WDRTS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207553 representing NM_007055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGGAGCAGTTCCGGGAGACGGATGTGGCCAAGAAAATAAGCCACATCTGTTTTGGAATGAAGT
  CACCTGAGGAGATGCGCCAGCAGGCGCACATCCAAGTTGTGAGTAAGAACCTGTACAGCCAGGACAACCA
  ACATGCCCCCTTGCTATATGGGGTGCTCGACCATAGGATGGGTACGAGTGAGAAGGATCGTCCATGTGAA
  ACCTGTGGGAAAAACTTGGCTGACTGTCTAGGCCACTATGGGTATATCGACCTGGAGTTGCCGTGTTTTC
  ATGTAGGGTACTTCAGAGCAGTCATAGGCATCTTACAGATGATCTGCAAAACCTGCTGCCACATCATGCT
  GTCCCAAGAGGAGAAGAAGCAGTTTCTGGACTATCTAAAGAGGCCCGGCCTGACCTACCTTCAGAAGCGA
  GGACTGAAAAAGAAAATCTCTGACAAGTGCCGGAAGAAAAACATCTGCCATCACTGTGGCGCTTTTAATG
  GTACCGTAAAGAAGTGTGGACTGCTGAAAATAATTCATGAGAAATACAAGACCAACAAAAAAGTGGTGGA
  TCCCATTGTATCAAATTTCCTTCAGTCTTTTGAAACAGCCATTGAACATAATAAAGAAGTGGAGCCTCTG
  CTGGGAAGGGCACAGGAAAACTTGAATCCCTTAGTAGTTCTGAATTTATTTAAACGAATCCCAGCTGAAG
  ATGTTCCTCTACTTCTGATGAACCCAGAAGCCGGAAAGCCGTCTGATTTGATTCTCACACGACTTTTGGT
  GCCTCCTTTGTGTATCAGACCCTCCGTTGTGAGTGATTTGAAGTCTGGCACCAATGAAGATGATCTGACA
  ATGAAACTGACAGAAATCATTTTCCTAAACGATGTTATTAAAAAGCATCGGATCTCAGGAGCCAAGACCC
  AGATGATCATGGAGGACTGGGATTTCCTGCAGCTGCAGTGTGCCCTCTACATTAACAGTGAGCTCTCGGG
  CATTCCCCTCAACATGGCACCCAAGAAGTGGACCAGAGGCTTCGTCCAACGCCTGAAGGGAAAACAGGGT
  CGATTTAGAGGAAATCTCTCAGGAAAGAGAGTGGATTTTTCTGGCAGAACAGTCATCTCGCCCGACCCCA
  ACCTCCGGATTGATGAGGTAGCTGTGCCAGTTCATGTGGCCAAAATTCTAACTTTTCCTGAGAAGGTAAA
  CAAAGCAAACATCAATTTCTTGAGGAAACTGGTTCAAAACGGCCCTGAGGTTCACCCAGGAGCAAACTTC
  ATTCAGCAGAGACATACGCAGATGAAAAGGTTTTTGAAATACGGAAATCGAGAAAAGATGGCTCAAGAGC
  TCAAGTATGGTGACATCGTAGAGAGACACCTCATCGATGGAGATGTGGTGCTGTTCAATCGGCAGCCCTC
  GCTGCACAAATTGAGCATTATGGCTCATCTGGCCAGGGTCAAGCCCCACCGGACCTTCAGATTTAATGAG
  TGTGTCTGTACACCCTATAATGCTGACTTTGATGGTGATGAAATGAACCTTCATCTTCCTCAAACAGAAG
  AAGCTAAAGCAGAGGCCCTTGTTCTGATGGGGACTAAAGCAAATCTTGTAACCCCGAGGAATGGGGAACC
  GCTGATTGCTGCTATTCAGGATTTTCTAACAGGTGCCTATCTCCTCACTCTCAAGGACACTTTCTTTGAT
  CGAGCCAAGGCTTGCCAAATCATTGCTTCAATACTGGTTGGCAAGGATGAGAAAATTAAAGTTCGCCTCC
  CACCGCCTACAATCCTAAAGCCTGTCACCCTGTGGACGGGAAAGCAGATCTTCAGTGTCATCCTCAGGCC
  TAGCGATGACAATCCAGTGAGGGCCAACCTGCGAACCAAGGGCAAGCAGTACTGTGGCAAAGGGGAAGAT
  CTCTGTGCCAATGATTCCTATGTTACAATCCAGAACAGTGAGTTGATGAGTGGCAGCATGGACAAAGGAA
  CCCTAGGGTCAGGATCCAAGAACAATATTTTTTACATTTTGCTGCGAGACTGGGGACAGAATGAAGCTGC
  AGATGCCATGTCACGGCTCGCCAGGCTGGCTCCTGTCTACCTGTCTAACCGTGGTTTCTCAATTGGGATC
  GGTGATGTCACACCTGGCCAAGGACTGCTGAAGGCCAAGTATGAGTTGCTGAATGCCGGCTACAAGAAAT
  GTGATGAGTACATCGAAGCCCTGAACACGGGCAAGCTGCAGCAGCAGCCTGGCTGCACTGCTGAGGAGAC
  CCTGGAGGCACTGATCCTGAAGGAGCTGTCTGTGATCCGTGACCACGCTGGCAGTGCCTGCCTCCGGGAG
  CTGGACAAGAGCAACAGCCCCCTCACCATGGCTCTGTGCGGCTCCAAAGGTTCCTTCATTAACATATCAC
  AGATGATTGCCTGTGTGGGACAGCAGGCCATCAGTGGCTCTCGAGTGCCAGACGGCTTTGAAAACAGGTC
  CTTGCCTCATTTTGAAAAACACTCAAAGCTCCCAGCTGCCAAAGGCTTTGTGGCTAATAGCTTTTATTCC
  GGTTTGACACCAACTGAGTTTTTCTTCCACACAATGGCCGGCCGGGAAGGTCTAGTCGACACGGCTGTAA
  AGACAGCTGAAACGGGATACATGCAGCGAAGGCTTGTCAAATCTCTTGAAGATCTTTGCTCCCAGTATGA
  TCTGACAGTCCGAAGCTCTACTGGCGATATTATCCAGTTCATTTATGGAGGAGATGGCTTAGATCCTGCA
  GCTATGGAGGGAAAAGATGAACCTTTGGAGTTTAAAAGGGTTCTGGACAACATCAAAGCAGTCTTCCCGT
  GTCCCAGTGAGCCTGCTCTCAGCAAAAACGAGCTGATCCTGACCACAGAGTCCATCATGAAGAAGAGTGA
  GTTCCTCTGCTGCCAGGACAGCTTCCTGCAGGAAATAAAAAAATTCATTAAGGGGGTCTCTGAGAAGATC
  AAGAAAACCAGAGATAAATATGGCATCAATGATAACGGCACAACAGAGCCCCGTGTGCTGTACCAGCTGG
  ACCGCATCACCCCCACCCAAGTAGAAAAGTTTCTGGAGACCTGTAGGGACAAGTACATGAGGGCACAGAT
  GGAGCCAGGTTCTGCAGTGGGTGCTCTGTGTGCCCAGAGCATTGGTGAGCCAGGCACCCAGATGACCCTG
  AAGACTTTCCACTTTGCAGGTGTGGCCTCCATGAACATCACCCTGGGCGTGCCCCGGATTAAAGAGATCA
  TCAACGCTTCCAAGGCCATCAGCACTCCAATTATCACAGCACAGCTAGACAAGGATGACGACGCGGATTA
  TGCTCGCCTCGTGAAAGGGAGAATTGAGAAAACCCTCTTGGGAGAGATTTCCGAGTATATTGAAGAAGTG
  TTTCTTCCTGATGACTGCTTTATTCTCGTCAAGCTCTCCCTGGAACGGATTAGGCTTCTGAGACTGGAAG
  TGAACGCTGAGACAGTGAGATATTCCATCTGCACATCCAAGCTCCGTGTGAAGCCCGGTGATGTGGCTGT
  TCATGGTGAGGCTGTGGTGTGTGTCACCCCCAGAGAGAACAGCAAGAGCTCCATGTACTACGTGCTGCAG
  TTCCTGAAAGAGGATCTCCCCAAGGTGGTGGTGCAGGGCATTCCAGAGGTGTCCAGAGCTGTCATCCACA
  TTGACGAGCAGAGTGGAAAGGAGAAGTACAAGCTTCTGGTGGAAGGTGATAACCTGCGGGCAGTCATGGC
  CACACACGGTGTGAAGGGCACCCGAACCACCTCCAATAACACCTATGAGGTGGAGAAAACTCTGGGCATC
  GAGGCCGCCCGGACAACGATCATCAATGAAATCCAGTACACCATGGTGAACCACGGCATGAGCATCGACA
  GGAGGCACGTGATGCTGCTCTCCGACCTCATGACCTACAAGGGTGAAGTCCTGGGCATCACTAGGTTTGG
  CCTGGCCAAGATGAAGGAGAGTGTGCTGATGCTGGCCTCCTTTGAGAAGACGGCTGACCATCTCTTTGAC
  GCTGCCTACTTCGGGCAGAAGGACTCTGTGTGTGGGGTGTCTGAGTGCATCATCATGGGAATCCCAATGA
  ACATTGGAACCGGGCTCTTCAAGCTGCTTCACAAGGCTGACAGGGACCCGAACCCTCCCAAGAGGCCCCT
  GATCTTCGACACAAATGAATTCCACATCCCCCTTGTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207553 representing NM_007055
  Red=Cloning site Green=Tags(s)
  
  MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLYGVLDHRMGTSEKDRPCE
  TCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKR
  GLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPL
  LGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLT
  MKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQG
  RFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPEVHPGANF
  IQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNE
  CVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFD
  RAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGED
  LCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGI
  GDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRE
  LDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYS
  GLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPA
  AMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKI
  KKTRDKYGINDNGTTEPRVLYQLDRITPTQVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTL
  KTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEV
  FLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQ
  FLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGI
  EAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFD
  AAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007055
• ORF Size: 4170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007055.4
• RefSeq Size: 6652 bp
• RefSeq ORF: 4173 bp
• Locus ID: 11128
• UniProt ID: O14802
• Cytogenetics: 10q22.3
• Domains: RNA_pol_Rpb1_2, RPOLA_N, RNA_pol_Rpb1_3, RNA_pol_Rpb1_1, RNA_pol_Rpb1_5, RNA_pol_Rpb1_4
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 156.1 kDa
• Gene Summary: The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]