BMAL1 (ARNTL) (NM_001030273) Human Tagged ORF Clone

CAT#: RC204464

ARNTL (Myc-DDK-tagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 3

Product Images

Circular map for RC204464

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  Western blot validation of overexpression lysate (Cat# LY422247) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204464 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ARNTL protein (Cat# TP304464). The protein was produced from HEK293T cells transfected with ARNTL cDNA clone (Cat# RC204464) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMAL1
• Synonyms: bHLHe5; BMAL1; BMAL1c; JAP3; MOP3; PASD3; TIC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204464 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAATATAGAAAGCATGGACACAGACAAAGATGACCCTCATGGAAGGTTAGAATATACAGAACACC
  AAGGAAGGATAAAAAATGCAAGGGAAGCTCACAGTCAGATTGAAAAGCGGCGTCGGGATAAAATGAACAG
  TTTTATAGATGAATTGGCTTCTTTGGTACCAACATGCAACGCAATGTCCAGGAAATTAGATAAACTTACT
  GTGCTAAGGATGGCTGTTCAGCACATGAAAACATTAAGAGGTGCCACCAATCCATACACAGAAGCAAACT
  ACAAACCAACTTTTCTATCAGACGATGAATTGAAACACCTCATTCTCAGGGCAGCAGATGGATTTTTGTT
  TGTCGTAGGATGTGACCGAGGGAAGATACTCTTTGTCTCAGAGTCTGTCTTCAAGATCCTCAACTACAGC
  CAGAATGATCTGATTGGTCAGAGTTTGTTTGACTACCTGCATCCTAAAGATATTGCCAAAGTCAAGGAGC
  AGCTCTCCTCCTCTGACACCGCACCCCGGGAGCGGCTCATAGATGCAAAAACTGGACTTCCAGTTAAAAC
  AGATATAACCCCTGGGCCATCTCGATTATGTTCTGGAGCACGACGTTCTTTCTTCTGTAGGATGAAGTGT
  AACAGGCCTTCAGTAAAGGTTGAAGACAAGGACTTCCCCTCTACCTGCTCAAAGAAAAAAGCAGATCGAA
  AAAGCTTCTGCACAATCCACAGCACAGGCTATTTGAAAAGCTGGCCACCCACAAAGATGGGGCTGGATGA
  AGACAACGAACCAGACAATGAGGGGTGTAACCTCAGCTGCCTCGTCGCAATTGGACGACTGCATTCTCAT
  GTAGTTCCACAACCAGTGAACGGGGAAATCAGGGTGAAATCTATGGAATATGTTTCTCGGCACGCGATAG
  ATGGAAAGTTTGTTTTTGTAGACCAGAGGGCAACAGCTATTTTGGCATATTTACCACAAGAACTTCTAGG
  CACATCGTGTTATGAATATTTTCACCAAGATGACATAGGACATCTTGCAGAATGTCATAGGCAAGTTTTA
  CAGACGAGAGAAAAAATTACAACTAATTGCTATAAATTTAAAATCAAAGATGGTTCTTTTATCACACTAC
  GGAGTCGATGGTTCAGTTTCATGAACCCTTGGACCAAGGAAGTAGAATATATTGTCTCAACTAACACTGT
  TGTTTTAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCACAGCTCACAGCATCCCCCCACAGCATG
  GACAGCATGCTGCCCTCTGGAGAAGGTGGCCCAAAGAGGACCCACCCCACTGTTCCAGGGATTCCAGGGG
  GAACCCGGGCTGGGGCAGGAAAAATAGGCCGAATGATTGCTGAGGAAATCATGGAAATCCACAGGATAAG
  AGGGTCATCGCCTTCTAGCTGTGGCTCCAGCCCATTGAACATCACGAGTACGCCTCCCCCTGATGCCTCT
  TCTCCAGGAGGCAAGAAGATTTTAAATGGAGGGACTCCAGACATTCCTTCCAGTGGCCTACTATCAGGCC
  AGGCTCAGGAGAACCCAGGTTATCCATATTCTGATAGTTCTTCTATTCTTGGTGAGAACCCCCACATAGG
  TATAGACATGATTGACAACGACCAAGGATCAAGTAGTCCCAGTAATGATGAGGCAGCAATGGCTGTCATC
  ATGAGCCTCTTGGAAGCAGATGCTGGACTGGGTGGCCCTGTTGACTTTAGTGACTTGCCATGGCCGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204464 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MINIESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLT
  VLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYS
  QNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKC
  NRPSVKVEDKDFPSTCSKKKADRKSFCTIHSTGYLKSWPPTKMGLDEDNEPDNEGCNLSCLVAIGRLHSH
  VVPQPVNGEIRVKSMEYVSRHAIDGKFVFVDQRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVL
  QTREKITTNCYKFKIKDGSFITLRSRWFSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTASPHSM
  DSMLPSGEGGPKRTHPTVPGIPGGTRAGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDAS
  SPGGKKILNGGTPDIPSSGLLSGQAQENPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVI
  MSLLEADAGLGGPVDFSDLPWPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030273
• ORF Size: 1749 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030273.2
• RefSeq Size: 2879 bp
• RefSeq ORF: 1749 bp
• Locus ID: 406
• UniProt ID: O00327
• Cytogenetics: 11p15.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 64.2 kDa
• Gene Summary: The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]