Alanine Transaminase (GPT) (NM_005309) Human Tagged ORF Clone

CAT#: RC203756

GPT (Myc-DDK-tagged)-Human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT)

Product Images

Circular map for RC203756

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  Western blot validation of overexpression lysate (Cat# LY417391) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203756 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GPT protein (Cat# TP303756). The protein was produced from HEK293T cells transfected with GPT cDNA clone (Cat# RC203756) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alanine Transaminase
• Synonyms: AAT1; ALT1; GPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203756 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGAGCACAGGTGACCGGAGCCAGGCGGTGAGGCATGGACTGAGGGCGAAGGTGCTGACGCTGG
  ACGGCATGAACCCGCGTGTGCGGAGAGTGGAGTACGCAGTGCGTGGCCCCATAGTGCAGCGAGCCTTGGA
  GCTGGAGCAGGAGCTGCGCCAGGGTGTGAAGAAGCCTTTCACCGAGGTCATCCGTGCCAACATCGGGGAC
  GCACAGGCTATGGGGCAGAGGCCCATCACCTTCCTGCGCCAGGTCTTGGCCCTCTGTGTTAACCCTGATC
  TTCTGAGCAGCCCCAACTTCCCTGACGATGCCAAGAAAAGGGCGGAGCGCATCTTGCAGGCGTGTGGGGG
  CCACAGTCTGGGGGCCTACAGCGTCAGCTCCGGCATCCAGCTGATCCGGGAGGACGTGGCGCGGTACATT
  GAGAGGCGTGACGGAGGCATCCCTGCGGACCCCAACAACGTCTTCCTGTCCACAGGGGCCAGCGATGCCA
  TCGTGACGGTGCTGAAGCTGCTGGTGGCCGGCGAGGGCCACACACGCACGGGTGTGCTCATCCCCATCCC
  CCAGTACCCACTCTACTCGGCCACGCTGGCAGAGCTGGGCGCAGTGCAGGTGGATTACTACCTGGACGAG
  GAGCGTGCCTGGGCGCTGGACGTGGCCGAGCTTCACCGTGCACTGGGCCAGGCGCGTGACCACTGCCGCC
  CTCGTGCGCTCTGTGTCATCAACCCTGGCAACCCCACCGGGCAGGTGCAGACCCGCGAGTGCATCGAGGC
  CGTGATCCGCTTCGCCTTCGAAGAGCGGCTCTTTCTGCTGGCGGACGAGGTGTACCAGGACAACGTGTAC
  GCCGCGGGTTCGCAGTTCCACTCATTCAAGAAGGTGCTCATGGAGATGGGGCCGCCCTACGCCGGGCAGC
  AGGAGCTTGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGCGGGTTCCGCGGCGGCTATGT
  GGAGGTGGTGAACATGGACGCTGCAGTGCAGCAGCAGATGCTGAAGCTGATGAGTGTGCGGCTGTGCCCG
  CCGGTGCCAGGACAGGCCCTGCTGGACCTGGTGGTCAGCCCGCCCGCGCCCACCGACCCCTCCTTTGCGC
  AGTTCCAGGCTGAGAAGCAGGCAGTGCTGGCAGAGCTGGCGGCCAAGGCCAAGCTCACCGAGCAGGTCTT
  CAATGAGGCTCCTGGCATCAGCTGCAACCCAGTGCAGGGCGCCATGTACTCCTTCCCGCGCGTGCAGCTG
  CCCCCGCGGGCGGTGGAGCGCGCTCAGGAGCTGGGCCTGGCCCCCGATATGTTCTTCTGCCTGCGCCTCC
  TGGAGGAGACCGGCATCTGCGTGGTGCCAGGGAGCGGCTTTGGGCAGCGGGAAGGCACCTACCACTTCCG
  GATGACCATTCTGCCCCCCTTGGAGAAACTGCGGCTGCTGCTGGAGAAGCTGAGCAGGTTCCATGCCAAG
  TTCACCCTCGAGTACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203756 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD
  AQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYI
  ERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDE
  ERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVY
  AAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCP
  PVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQL
  PPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAK
  FTLEYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005309
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005309.3
• RefSeq Size: 1896 bp
• RefSeq ORF: 1491 bp
• Locus ID: 2875
• UniProt ID: P24298
• Cytogenetics: 8q24.3
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways
• MW: 54.6 kDa
• Gene Summary: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]