Rasgrp2 (NM_011242) Mouse Tagged ORF Clone

CAT#: MR226786

Rasgrp2 (Myc-DDK-tagged) - Mouse RAS, guanyl releasing protein 2 (Rasgrp2)

Product Images

Circular map for MR226786

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rasgrp2
• Synonyms: Caldaggef1; CDC25L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226786 representing NM_011242
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAGCACTCTGGACCTGGACAAGGGCTGCACCGTGGAGGAGCTGCTCCGTGGTTGCATCGAAGCGT
  TTGATGACTCCGGAAAGGTGCGAGATCCACAGCTAGTGCGCATGTTTCTCATGATGCACCCCTGGTACAT
  ACCTTCCTCTCAGCTGGCTTCGAAACTGCTCCACTTCTATCAGCAATCCCGGAAGGACAACTCCAATTCT
  CTACAGATGAAAACGTGTCACTTGGTCAGGTACTGGATCTCAGCCTTCCCAGCAGAGTTCGACTTGAACC
  CAGAGCTGGCTGAACAGATCAAGGAGCTGAAGGCTCTGTTAGACCAAGAAGGGAACCGCAGGCACAGCAG
  CCTCATCGACATCGAGAGTGTCCCTACCTACAAATGGAAGCGGCAGGTGACCCAGCGGAACCCTGTGGAA
  CAGAAAAAGCGCAAGATGTCCTTGTTGTTTGATCACTTGGAGCCTATGGAGCTGGCAGAACATCTTACCT
  ACCTGGAGTATCGTTCCTTCTGCAAGATCCTGTTCCAAGACTACCACAGCTTTGTGACTCATGGCTGCAC
  CGTAGACAACCCGGTCCTGGAGCGATTCATCTCCCTCTTCAACAGTGTCTCTCAGTGGGTCCAACTCATG
  ATCCTCAGCAAGCCCACAGCCACGCAGCGGGCGCTGGTCATCACGCACTTCGTGCATGTGGCAGAGAGGC
  TGCTGCAGCTGCAGAACTTCAACACGCTGATGGCCGTCGTGGGAGGCCTGAGCCACAGCTCCATCTCACG
  CCTCAAGGAGACCCACAGCCACGTCAGCCCTGACACCATCAAGCTCTGGGAAGGTCTGACAGAACTAGTG
  ACAGCTACTGGCAACTACAGCAACTACCGGCGCAGGCTGGCAGCCTGCGTGGGCTTCCGCTTTCCTATCC
  TGGGAGTGCACCTCAAGGATCTAGTGGCTCTGCAGCTGGCTCTGCCTGACTGGCTGGACCCAGGTCGGAC
  CCGGCTCAATGGAGCCAAGATGAGGCAGCTTTTCTGCATTCTGGAGGAGTTGGCCATGGTGACCAGCCTT
  CGCCCACCCGTACAAGCCAACCCCGACCTGCTGAGCCTGCTCACGGTGTCCCTGGATCAGTATCAGACAG
  AGGATGAGCTGTACCAGCTCTCACTGCAGCGAGAGCCACGCTCCAAGTCGTCGCCAACCAGCCCCACCAG
  CTGCACCCCGCCTCCCCGGCCCCCTGTGTTGGAAGAGTGGACCTCAGTTGCCAAGCCTAAGCTGGACCAA
  GCCTTGGTGGCAGAGCACATTGAGAAGATGGTGGAGTCTGTGTTCCGGAACTTTGACGTGGATGGGGACG
  GTCACATCTCCCAGGAGGAGTTTCAGATCATCCGGGGCAACTTCCCTTATCTCAGCGCCTTTGGGGACCT
  GGACCAGAACCAGGATGGCTGCATCAGCCGGGAGGAGATGATTTCCTACTTCCTGCGCTCCAGCTCCGTG
  CTGGGAGGCCGCATGGGCTTCGTACACAACTTCCAGGAGAGTAACTCGCTACGCCCGGTTGCCTGTCGCC
  ACTGCAAAGCTCTGATCCTGGGCATCTACAAGCAGGGCCTTAAATGTAGAGCTTGTGGTGTGAACTGCCA
  CAAGCAATGCAAAGAGCGCCTGTCAGTGGAATGTCGCCGCAGGGCCCAGAGTGTCAGCCTGGAGGGCTCT
  GCACCCTCTCCCTCACCCACACATACCCACCATCGGGCCTTCAGCTTTTCCCTGCCTCGCCCAGGCAGGC
  GCAGCTCTCGGCCTCCAGAGATCCGTGAAGAGGAGGTGCAGTCGGTGGAGGACGGAGTGTTTGACATCCA
  CTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226786 representing NM_011242
  Red=Cloning site Green=Tags(s)
  
  MTSTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLASKLLHFYQQSRKDNSNS
  LQMKTCHLVRYWISAFPAEFDLNPELAEQIKELKALLDQEGNRRHSSLIDIESVPTYKWKRQVTQRNPVE
  QKKRKMSLLFDHLEPMELAEHLTYLEYRSFCKILFQDYHSFVTHGCTVDNPVLERFISLFNSVSQWVQLM
  ILSKPTATQRALVITHFVHVAERLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPDTIKLWEGLTELV
  TATGNYSNYRRRLAACVGFRFPILGVHLKDLVALQLALPDWLDPGRTRLNGAKMRQLFCILEELAMVTSL
  RPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSPTSPTSCTPPPRPPVLEEWTSVAKPKLDQ
  ALVAEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSAFGDLDQNQDGCISREEMISYFLRSSSV
  LGGRMGFVHNFQESNSLRPVACRHCKALILGIYKQGLKCRACGVNCHKQCKERLSVECRRRAQSVSLEGS
  APSPSPTHTHHRAFSFSLPRPGRRSSRPPEIREEEVQSVEDGVFDIHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011242
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011242.3
• RefSeq Size: 2209 bp
• RefSeq ORF: 1827 bp
• Locus ID: 19395
• UniProt ID: Q9QUG9
• Cytogenetics: 19 A
• MW: 69.4 kDa
• Gene Summary: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.[UniProtKB/Swiss-Prot Function]