Clock (NM_007715) Mouse Tagged ORF Clone

CAT#: MR226315

Clock (Myc-DDK-tagged) - Mouse circadian locomotor output cycles kaput (Clock)

Product Images

Circular map for MR226315

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clock
• Synonyms: 5330400M04Rik; KAT13D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226315 representing NM_007715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTACCGTAAGCTGTAGTAAAATGAGCTCAATTGTTGACAGAGATGACAGTAGTATTTTTGATG
  GATTGGTGGAAGAAGATGACAAGGACAAAGCAAAAAGAGTATCTAGAAACAAATCAGAAAAGAAACGTAG
  AGATCAGTTCAATGTCCTCATTAAGGAGCTGGGGTCTATGCTTCCTGGTAACGCGAGAAAGATGGACAAG
  TCTACTGTTCTACAGAAGAGCATTGATTTTTTGCGCAAACATAAAGAGACCACTGCACAGTCAGATGCTA
  GTGAGATTCGACAGGACTGGAAACCCACATTCCTTAGTAATGAAGAGTTTACACAGTTAATGTTAGAGGC
  TCTTGATGGTTTTTTTTTAGCGATCATGACAGATGGAAGTATAATATATGTATCTGAGAGTGTAACTTCG
  TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAGGGAGAACATT
  CAGAGGTTTATAAGATACTCTCTACTCATCTGCTGGAAAGTGACTCATTAACCCCTGAGTACTTAAAATC
  AAAAAATCAGTTAGAATTCTGTTGTCACATGCTTCGAGGAACAATAGACCCAAAGGAGCCATCCACCTAT
  GAATATGTGAGATTTATAGGAAATTTTAAATCTTTAACCAGTGTATCAACTTCAACACACAATGGTTTTG
  AAGGAACTATACAACGCACACATAGGCCTTCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGATT
  AGCTACACCTCAGTTCATCAAGGAAATGTGTACTGTTGAAGAACCAAATGAAGAGTTTACATCTAGACAC
  AGTTTAGAATGGAAGTTTCTATTTTTAGATCACAGGGCACCACCAATAATAGGCTATTTGCCATTTGAAG
  TCTTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATCTGGCAAAATGTCACGAGCA
  CTTAATGCAATATGGAAAAGGCAAATCGTGTTACTATAGATTCCTGACCAAAGGCCAGCAGTGGATATGG
  CTTCAGACTCATTATTATATTACTTACCATCAGTGGAATTCAAGGCCAGAGTTCATTGTTTGTACTCACA
  CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGGCGAGAACTTGGCATTGAAGAGTCTCTTCCTGA
  GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCTGACAATCGTATCAACACAGTGAGTCTCAAGGAAGCA
  CTGGAAAGGTTTGATCACAGCCCAACTCCTTCTGCCTCCTCTAGAAGCTCACGAAAGTCATCTCACACCG
  CAGTCTCAGACCCTTCCTCCACACCGACAAAGATCCCTACTGATACTAGCACTCCTCCCAGACAGCATTT
  GCCAGCTCATGAAAAGATGACACAGCGGAGGTCGTCCTTCAGCAGTCAGTCCATAAACTCCCAGTCAGTT
  GGTCCATCATTAACACAGCCAGCGATGTCTCAAGCTGCAAATTTACCAATTCCACAAGGCATGTCACAGT
  TTCAGTTTTCAGCTCAGTTAGGAGCCATGCAGCATCTAAAAGACCAGCTAGAGCAGCGGACACGGATGAT
  AGAGGCAAATATTCATCGGCAGCAAGAAGAACTAAGGAAAATTCAAGAGCAACTTCAGATGGTCCATGGT
  CAAGGGCTACAGATGTTTTTGCAGCAATCAAACCCTGGATTGAATTTTGGTTCTGTTCAACTTTCCTCTG
  GAAATTCTAATATCCAGCAACTCACACCTGTAAATATGCAAGGCCAGGTTGTCCCTGCTAACCAGGTTCA
  GAGTGGACATATCAGCACAGGCCAGCACATGATACAGCAACAGACTTTACAAAGTACATCAACTCAGCAG
  AGTCAACAGAGTGTAATGAGTGGACACAGTCAGCAGACGTCTCTTCCAAGTCAGACACCGAGCACTCTCA
  CAGCCCCACTGTACAATACGATGGTGATTTCCCAGCCTGCAGCTGGGAGCATGGTCCAGATTCCATCCAG
  TATGCCACAGAACAGTACCCAGAGTGCTACAGTCACTACGTTCACTCAGGACAGACAGATAAGATTTTCT
  CAAGGTCAGCAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCCGTCATGGTACCAAGTA
  CCATGCTTATGGGTCAGGTGGTGACTGCCTATCCTACCTTCGCCACACAACAGCAGCAGGCACAGACATT
  ATCGGTAACACAACAGCAGCAGCAGCAGCAGCAGCAGCCACCACAGCAACAGCAACAACAACAGCAGAGT
  TCCCAGGAACAGCAGCTTCCTTCAGTTCAGCAGCCAGCTCAGGCCCAGCTGGGCCAGCCACCACAGCAGT
  TCTTACAGACATCTAGGTTGCTCCACGGGAATCCTTCGACACAGCTCATCCTCTCTGCTGCCTTTCCACT
  ACAACAGAGCACTTTCCCTCCTTCGCACCACCAGCAACACCAGCCTCAGCAGCAACAGCAGCTTCCTCGG
  CACAGGACTGACAGCCTGACTGACCCTTCCAAGGTCCAGCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226315 representing NM_007715
  Red=Cloning site Green=Tags(s)
  
  MVFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDK
  STVLQKSIDFLRKHKETTAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTS
  LLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTY
  EYVRFIGNFKSLTSVSTSTHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRH
  SLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIW
  LQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKSQDSGSDNRINTVSLKEA
  LERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRQHLPAHEKMTQRRSSFSSQSINSQSV
  GPSLTQPAMSQAANLPIPQGMSQFQFSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHG
  QGLQMFLQQSNPGLNFGSVQLSSGNSNIQQLTPVNMQGQVVPANQVQSGHISTGQHMIQQQTLQSTSTQQ
  SQQSVMSGHSQQTSLPSQTPSTLTAPLYNTMVISQPAAGSMVQIPSSMPQNSTQSATVTTFTQDRQIRFS
  QGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQAQTLSVTQQQQQQQQQPPQQQQQQQQS
  SQEQQLPSVQQPAQAQLGQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPPSHHQQHQPQQQQQLPR
  HRTDSLTDPSKVQPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007715
• ORF Size: 2565 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007715.6
• RefSeq Size: 7478 bp
• RefSeq ORF: 2568 bp
• Locus ID: 12753
• UniProt ID: O08785
• Cytogenetics: 5 40.63 cM
• MW: 96.8 kDa
• Gene Summary: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2014]