Cfb (NM_008198) Mouse Tagged ORF Clone

CAT#: MR226025

Cfb (Myc-DDK-tagged) - Mouse complement factor B (Cfb), transcript variant 1

Product Images

Circular map for MR226025

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfb
• Synonyms: AI195813; AI255840; B; Bf; C2; Fb; H2-Bf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226025 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATGGAGAGCCCCCAGCTCTGCCTCGTCCTCTTGGTCTTAGGCTTCTCCTCTGGAGGTGTGAGCG
  CAACTCCAGTGCTTGAGGCCCGGCCCCAAGTCTCCTGCTCTCTGGAGGGAGTAGAGATCAAAGGCGGCTC
  CTTTCAACTTCTCCAAGGCGGTCAGGCCCTGGAGTACCTATGTCCCTCTGGCTTCTACCCATACCCCGTG
  CAGACTCGAACCTGCAGATCCACAGGCTCCTGGAGCGACCTGCAGACCCGAGACCAAAAGATTGTCCAGA
  AGGCGGAATGCAGAGCAATACGCTGCCCACGACCGCAGGACTTTGAAAATGGGGAATTCTGGCCCCGGTC
  CCCCTTCTACAACCTGAGTGACCAGATTTCTTTTCAATGCTATGATGGTTACGTTCTCCGGGGCTCTGCT
  AATCGCACCTGCCAAGAGAATGGCCGGTGGGATGGGCAAACAGCAATTTGTGATGATGGAGCTGGATACT
  GTCCCAATCCCGGTATTCCTATTGGGACAAGGAAGGTGGGTAGCCAATACCGCCTTGAAGACATTGTTAC
  TTACCACTGCAGCCGGGGACTTGTCCTGCGTGGCTCCCAGAAGCGAAAGTGTCAAGAAGGTGGCTCATGG
  AGTGGGACAGAGCCTTCCTGCCAAGATTCCTTCATGTATGACAGCCCTCAAGAAGTGGCCGAAGCATTCC
  TATCCTCCCTGACAGAGACCATCGAAGGAGCCGATGCTGAGGATGGGCACAGCCCAGGAGAACAGCAGAA
  GAGGAAGATTGTCCTAGACCCCTCGGGCTCCATGAATATCTACCTGGTGCTAGATGGATCAGACAGCATC
  GGAAGCAGCAACTTCACAGGGGCTAAGCGGTGCCTCACCAACTTGATTGAGAAGGTGGCGAGTTACGGGG
  TGAGGCCACGATATGGTCTCCTGACATATGCTACAGTCCCCAAAGTGTTGGTCAGAGTGTCTGATGAGAG
  GAGTAGCGATGCCGACTGGGTCACAGAGAAGCTCAACCAAATCAGTTATGAAGACCACAAGCTGAAGTCA
  GGGACCAACACCAAGAGGGCTCTCCAGGCTGTGTATAGCATGATGAGCTGGGCAGGGGATGCCCCGCCTG
  AAGGCTGGAATAGAACCCGCCATGTCATCATCATTATGACTGATGGCTTGCACAACATGGGTGGAAACCC
  TGTCACTGTCATTCAGGACATCCGAGCCTTGCTGGACATCGGCAGGGATCCCAAAAATCCCAGGGAGGAT
  TACCTGGATGTGTATGTGTTTGGGGTCGGGCCTCTGGTGGACTCCGTGAACATCAATGCCTTAGCTTCCA
  AAAAGGACAATGAGCATCATGTGTTTAAAGTCAAGGATATGGAAGACCTGGAGAATGTTTTCTACCAAAT
  GATTGATGAAACCAAATCTCTGAGTCTCTGTGGCATGGTGTGGGAGCATAAAAAAGGCAACGATTATCAT
  AAGCAACCATGGCAAGCCAAGATCTCAGTCACTCGCCCTCTGAAAGGACATGAGACCTGTATGGGGGCCG
  TGGTGTCTGAGTACTTCGTGCTGACAGCAGCGCACTGCTTCATGGTGGATGATCAGAAACATTCCATCAA
  GGTCAGCGTGGGGGGTCAGAGGCGGGACCTGGAGATTGAAGAGGTCCTGTTCCACCCCAAATACAATATT
  AATGGGAAAAAGGCAGAAGGGATCCCTGAGTTCTATGATTATGATGTGGCCCTAGTCAAGCTCAAGAACA
  AGCTCAAGTATGGCCAGACTCTCAGGCCCATCTGTCTCCCCTGCACGGAGGGAACCACACGAGCCTTGAG
  GCTTCCTCAGACAGCCACCTGCAAGCAGCACAAGGAACAGTTGCTCCCTGTGAAGGATGTCAAAGCTCTG
  TTTGTATCTGAGCAAGGGAAGAGCCTGACTCGGAAGGAGGTGTACATCAAGAATGGGGACAAGAAAGCCA
  GTTGTGAGAGAGATGCTACAAAGGCCCAAGGCTATGAGAAGGTCAAAGATGCCTCTGAGGTGGTCACTCC
  ACGGTTCCTCTGCACAGGAGGGGTGGATCCCTATGCTGACCCCAACACATGCAAAGGAGATTCCGGGGGC
  CCTCTCATTGTTCACAAGAGAAGCCGCTTCATTCAAGTTGGTGTGATTAGCTGGGGAGTAGTAGATGTCT
  GCAGAGACCAGAGGCGGCAACAGCTGGTACCCTCTTATGCCCGGGACTTCCACATCAACCTCTTCCAGGT
  GCTGCCCTGGCTAAAGGACAAGCTCAAAGATGAGGATTTGGGTTTTCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226025 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTMESPQLCLVLLVLGFSSGGVSATPVLEARPQVSCSLEGVEIKGGSFQLLQGGQALEYLCPSGFYPYPV
  QTRTCRSTGSWSDLQTRDQKIVQKAECRAIRCPRPQDFENGEFWPRSPFYNLSDQISFQCYDGYVLRGSA
  NRTCQENGRWDGQTAICDDGAGYCPNPGIPIGTRKVGSQYRLEDIVTYHCSRGLVLRGSQKRKCQEGGSW
  SGTEPSCQDSFMYDSPQEVAEAFLSSLTETIEGADAEDGHSPGEQQKRKIVLDPSGSMNIYLVLDGSDSI
  GSSNFTGAKRCLTNLIEKVASYGVRPRYGLLTYATVPKVLVRVSDERSSDADWVTEKLNQISYEDHKLKS
  GTNTKRALQAVYSMMSWAGDAPPEGWNRTRHVIIIMTDGLHNMGGNPVTVIQDIRALLDIGRDPKNPRED
  YLDVYVFGVGPLVDSVNINALASKKDNEHHVFKVKDMEDLENVFYQMIDETKSLSLCGMVWEHKKGNDYH
  KQPWQAKISVTRPLKGHETCMGAVVSEYFVLTAAHCFMVDDQKHSIKVSVGGQRRDLEIEEVLFHPKYNI
  NGKKAEGIPEFYDYDVALVKLKNKLKYGQTLRPICLPCTEGTTRALRLPQTATCKQHKEQLLPVKDVKAL
  FVSEQGKSLTRKEVYIKNGDKKASCERDATKAQGYEKVKDASEVVTPRFLCTGGVDPYADPNTCKGDSGG
  PLIVHKRSRFIQVGVISWGVVDVCRDQRRQQLVPSYARDFHINLFQVLPWLKDKLKDEDLGFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008198
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008198.2, NP_032224.2
• RefSeq Size: 2767 bp
• RefSeq ORF: 2292 bp
• Locus ID: 14962
• Cytogenetics: 17 18.41 cM
• MW: 85.2 kDa